Strain-Specific Transfer of Antibiotic Resistance from an Environmental Plasmid to Foodborne Pathogens

Meervenne, Eva Van; Coillie, Els Van; Kerckhof, Frederiek‐Maarten; Devlieghere, Frank; Herman, Lieve; Gelder, Leen S. P. De; Top, Eva M.; Boon, Nico

doi:10.1155/2012/834598

Cited by 37 publications

(38 citation statements)

References 36 publications

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“…According to Ramsden et al (2010), class 1 integrons appeared to be ubiquitous among tetracycline-resistant bacterial isolates. Van Meervenne et al (2012) observed that an environmental plasmid can be transferred to foodborne pathogenic bacteria at high transfer ratios. In our study, the frequency of conjugation ranged from 2.0 × 10 −6 to 6.5 × 10 −5 per donor strain.…”

Section: Culture-based Approachmentioning

confidence: 99%

tet genes as indicators of changes in the water environment: Relationships between culture-dependent and culture-independent approaches

Harnisz

Korzeniewska

Ciesielski

et al. 2015

Science of The Total Environment

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Section: Culture-based Approachmentioning

confidence: 99%

tet genes as indicators of changes in the water environment: Relationships between culture-dependent and culture-independent approaches

Harnisz

Korzeniewska

Ciesielski

et al. 2015

Science of The Total Environment

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“…ARGs are often carried on broad host range mobile genetic elements (MGEs) (Byrne-Bailey et al 2011;Akiyama et al 2010;Binh et al 2008), which are of particular concern because these vectors may be disseminated from environmental hotspots through water and food webs into clinically relevant pathogenic or opportunistic bacteria (Van Meervenne et al 2012;Jayaraman 2009). Benveniste and Davies (1973) first suggested a link between environmental and clinical AR after detecting high similarities between gentamicin-resistant enzymes from soil-associated Actinomycetes and enzymes that confer the same resistance in human pathogens such Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus aureus.…”

Section: Persistence Of Pharmaceuticals In Effluents and Twwirrigatedmentioning

confidence: 99%

Performance of the Municipal Wastewater Treatment Plant for Removal of Listeria monocytogenes

2015

Wastewater and Public Health

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The reuse of treated wastewater (TWW) for irrigation is a practical solution for overcoming water scarcity, especially in arid and semiarid regions of the world. However, there are several potential environmental and health-related risks associated with this practice. One such risk stems from the fact that TWW irrigation may increase antibiotic resistance (AR) levels in soil bacteria, potentially contributing to the global propagation of clinical AR. Wastewater treatment plant (WWTP) effluents have been recognized as significant environmental AR reservoirs due to selective pressure generated by antibiotics and other compounds that are frequently detected in effluents. This review summarizes a myriad of recent studies that have assessed the impact of anthropogenic practices on AR in environmental bacterial communities, with specific emphasis on elucidating the potential effects of TWW irrigation on AR in the soil microbiome. Based on the current state of the art, we conclude that contradictory to freshwater environments where WWTP effluent influx tends to expand antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes levels, TWW irrigation does not seem to impact AR levels in the soil microbiome. Although this conclusion is a cause for cautious optimism regarding the future implementation of TWW irrigation, we conclude that further studies aimed at assessing the scope of horizontal gene transfer between effluent-associated ARB and soil bacteria need to be further conducted before ruling out the possible contribution of TWW irrigation to antibiotic-resistant reservoirs in irrigated soils.

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“…Plasmid pB10 was previously tagged with mini-Tn5-P A1-04/03 ::gfp, a Tn5 derivative transposon encoding green fluorescent protein (GFP) to produce pB10::gfp (24). The persistence of pB10::gfp was determined in Pseudomonas putida H2 (15), P. putida UWC-1 (25), and P. veronii S34 (26).…”

Section: Methodsmentioning

confidence: 99%

Flow Cytometry and Real-Time Quantitative PCR as Tools for Assessing Plasmid Persistence

Loftie-Eaton

Tucker

Norton

et al. 2014

Appl Environ Microbiol

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The maintenance of a plasmid in the absence of selection for plasmid-borne genes is not guaranteed. However, plasmid persistence can evolve under selective conditions. Studying the molecular mechanisms behind the evolution of plasmid persistence is key to understanding how plasmids are maintained under nonselective conditions. Given the current crisis of rapid antibiotic resistance spread by multidrug resistance plasmids, this insight is of high medical relevance. The conventional method for monitoring plasmid persistence (i.e., the fraction of plasmid-containing cells in a population over time) is based on cultivation and involves differentiating colonies of plasmid-containing and plasmid-free cells on agar plates. However, this technique is timeconsuming and does not easily lend itself to high-throughput applications. Here, we present flow cytometry (FCM) and real-time quantitative PCR (qPCR) as alternative tools for monitoring plasmid persistence. For this, we measured the persistence of a model plasmid, pB10::gfp, in three Pseudomonas hosts and in known mixtures of plasmid-containing and -free cells. We also compared three performance criteria: dynamic range, resolution, and variance. Although not without exceptions, both techniques generated estimates of overall plasmid loss rates that were rather similar to those generated by the conventional plate count (PC) method. They also were able to resolve differences in loss rates between artificial plasmid persistence assays. Finally, we briefly discuss the advantages and disadvantages for each technique and conclude that, overall, both FCM and real-time qPCR are suitable alternatives to cultivation-based methods for routine measurement of plasmid persistence, thereby opening avenues for high-throughput analyses.

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Strain-Specific Transfer of Antibiotic Resistance from an Environmental Plasmid to Foodborne Pathogens

Cited by 37 publications

References 36 publications

tet genes as indicators of changes in the water environment: Relationships between culture-dependent and culture-independent approaches

tet genes as indicators of changes in the water environment: Relationships between culture-dependent and culture-independent approaches

Performance of the Municipal Wastewater Treatment Plant for Removal of Listeria monocytogenes

Flow Cytometry and Real-Time Quantitative PCR as Tools for Assessing Plasmid Persistence

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