Strain-specific changes in the gut microbiota profiles of the white shrimp Litopenaeus vannamei in response to cold stress

Li, Jiajia; Wang, Kai; Wang, Yanting; Chen, Wei; Jin, Zhongwen; Yao, Zhiyuan; Zhang, Demin

doi:10.1016/j.aquaculture.2019.01.026

Cited by 52 publications

(37 citation statements)

References 74 publications

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“…While present literature provides information on some identified differentially abundant RSVs, functions of other unreported or understudied RSVs are still currently unknown and are thus interesting to explore once available. Although the V4 region of the 16S rRNA gene is a widely used hypervariable marker in several shrimp microbiome studies (Liu et al., 2019; Xiong et al, 2015), other markers may also be used to give more useful information on the changes in the bacterial community profile on shrimp.…”

Section: Discussionmentioning

confidence: 99%

Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5‐aminolevulinic acid supplementation

et al. 2020

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Changes in the diet have been previously reported to alter the gut bacterial profile in several organisms, including shrimp. These shifts in microbial structure either promote beneficial effects to the host or cause diseases. Supplementation of 5‐aminolevulinic acid (5‐ALA), the precursor of tetrapyrroles, has been previously reported to enhance shrimp's immune response. To know whether 5‐ALA has effects on the gut bacterial structure in Pacific white shrimp (Litopenaeus vannamei), we investigated the bacterial communities in the intestine and stomach of L. vannamei using high‐throughput Illumina sequencing of 16S rRNA gene libraries. One week of 5‐ALA supplementation altered the bacterial community structure (beta diversity) in both tissues, as shown by the results of multi‐dimensional scaling (MDS) plots and analysis of similarities (ANOSIM). DESeq2 analysis revealed enrichment of differentially abundant taxa in the 5‐ALA group (e.g. Enhydrobacter and Oceaniovalibus) and control group (e.g. Tenacibaculum and Mycobacterium). Metagenomic predictions suggested that the control group had more KEGG pathways associated with ‘metabolism’ than the 5‐ALA group. This study suggests that 5‐ALA supplementation potentially promotes the formation of a beneficial bacterial community structure in shrimp. This is the first report on the effect of 5‐ALA supplementation on the bacterial community profile in any organism.

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Section: Discussionmentioning

confidence: 99%

Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5‐aminolevulinic acid supplementation

et al. 2020

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“…A study on the stress tolerance and its relationship with gut microbiome in two major L. vannamei strains in southeastern China, ZT (parent shrimp from Thailand) and PM (parent shrimp from the United States) revealed that the cold stress led to the dominance of members of Erysipelotrichaceae, Mycoplasmataceae and Vibrionaceae, which were opportunistic pathogens in both the strains. The family Rhodobacteraceae having a great probiotic potential was also abundant in PM strains as compared to ZT (Liu et al 2019). Salinity stress has a strong influence on the gut microbiome of L. vannamei.…”

Section: Environmental Stressmentioning

confidence: 99%

“…The most prominent factors which affect the gut microbiome easily and significantly are the exogenous factors or environmental changes like diet habits (Sullam et al 2015), and temperature (Liu et al 2019), etc.…”

Section: Exogenous Factorsmentioning

confidence: 99%

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Healthy microbiome: a key to successful and sustainable shrimp aquaculture

Rajeev

Adithya

Kiran

et al. 2020

Reviews in Aquaculture

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The microbiome of the host and its surroundings play a vital role in its health and well-being. Unlike the terrestrial organisms that maternally acquire its microbiome, the aquatic species mainly get colonized by the microorganisms in its aquatic surrounding and are exposed to a wide variety of microbial community based on the type of aquatic environment the host inhabits. This review is focused on the aquaculture and shrimp microbiome and factors that influence its microbiome such as prebiotics, probiotics, synbiotics, diet, stress conditions, frequency and type of antibiotics used, host physiological conditions and developmental stages. This review also casts light on the role of the microbiome in enhancing the shrimp health, immune response, microbial colonization, and the effects of the biofloc system on shrimp and aquaculture microbiome. Moreover, beneficial microbes can be a safer alternative to harmful antibiotics used in aquaculture, which can thereby prevent the emergence of superbugs and also enhance the production rate. A proper understanding of the interactions between the host and its microbiome can help us to modulate and manipulate the aquaculture microbiome to enhance the health of aquatic organisms, which ensures the growth of the aquaculture industry. Therefore, the treatment strategies should be targeted towards the modulation of shrimp and aquaculture microbiome, which acts as beneficial partners to overcome the challenges confronted by the aquaculture sector.

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“…In the marine environments, they comprise about 30 % of pelagic microbial communities as key players in the circulation of biochemical resources and symbiotic relationships with various marine organisms and are often found in marine eukaryote as mutualists [1, 3]. Shrimps also have a symbiotic relationship with bacterial communities and high abundance of family Rhodobacteraceae was observed in the gut microbiome of Litopenaeus vannamei strains that have resistance for cold-stress [4]. A strain belonging to this family was isolated during a study to investigate the microbial community in the gut of shrimp Litopenaeus vannamei , an important species in aquaculture industry [5].…”

Section: Full-textmentioning

confidence: 99%

Pukyongiella litopenaei gen. nov., sp. nov., a novel bacterium isolated from the gut content of a whiteleg shrimp Litopenaeus vannamei

Kim

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, aerobic bacterium, designated as SH-1T, was isolated from the gut content of a whiteleg shrimp, Litopenaeus vannamei collected in a shrimp farm in South Korea. The bacterial cells were ovoid rod-shaped, non-motile, oxidase-positive and catalase-negative. Growth was observed at 20–35 °C (optimum, 30 °C), pH 5.0–9.5 (pH 8.5) and in the presence of 0–6 % (w/v) NaCl (2–3 %). The major polar lipids were phosphatidylglycerol, phosphatidylinositolmannoside, unidentified aminolipid and two unidentified lipids. The G+C content was 66.1 mol% and the predominant respiratory quinone was Q-10. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SH-1T was placed in a distinct clade with Primorskyibacter marinus PX7T (96.97 % sequence similarity), Pontibaca methylaminivorans DSM 21219T (96.03 %) and Pelagivirga sediminicola BH-SD19T (95.02 %) in the family Rhodobacteraceae and distantly related with them to be a new genus. The digital DNA–DNA hybridization (dDDH), average nucleotide identity (ANI) and average amino acid identity (AAI) values calculated from whole-genome-sequence comparison between the SH-1T and the close species were in the ranges of 19.0–19.8, 73.8–74.9 and 64.1–65.9 %, respectively. Based on the polyphasic analysis presented in this study, we suggest that strain SH-1T represents a novel genus and species in the family Rhodobacteraceae , for which the name Pukyongiella litopenaei gen. nov., sp. nov. is proposed. The type strain of Pukyongiella litopenaei is SH-1T (=KCTC 62276T=MCCC 1K04072T).

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Strain-specific changes in the gut microbiota profiles of the white shrimp Litopenaeus vannamei in response to cold stress

Cited by 52 publications

References 74 publications

Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5‐aminolevulinic acid supplementation

Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5‐aminolevulinic acid supplementation

Healthy microbiome: a key to successful and sustainable shrimp aquaculture

Pukyongiella litopenaei gen. nov., sp. nov., a novel bacterium isolated from the gut content of a whiteleg shrimp Litopenaeus vannamei

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