Storage protocol of dental pulp cells from human exfoliated deciduous teeth.

Abstract

<p><strong>Objective:</strong> This study aimed to isolate the cells from the dental pulp tissue of human primary teeth, study the capacity of proliferation, characterize the cells and standardize the technique of culture and expansion to create a cell banking. <strong>Material and Methods:</strong> Primary teeth with no caries and orthodontic reasons were extracted for pulp tissue obtainment. The cells were extracted from the pulp cells, isolated and cultured under ideal conditions until full expansion. <strong>Results:</strong> After consecutive passages, the cultured cells were characterized using immunofluorescence technique and frozen between the 2<sup>nd</sup> and 6<sup>th</sup> passage, thus creating a biorepository of dental pulp cells from human primary teeth. <strong>Conclusion:</strong> The creation of a cell banking from dental pulp cells from human primary teeth enables the easy application of cells in laboratorial studies, reducing the cost and time for obtaining the samples, avoid the involvement of new subjects and allow a fast reproducibility of the researches.</p><p><strong> </strong></p><p><strong>Keywords</strong></p><p>Cell culture techniques; Tooth, deciduous; Pulp; Fibroblasts; Cryopreservation.</p>

Photobiomodulation changes type 1 collagen gene expression by pulp fibroblasts

“…Pulp fibroblasts from human primary teeth (HPF) were obtained through a Biorepository [15,23]. 1 × 10 5 cells/ well were seeded in 24-well plate (code 3524, polystyrene, Corning, NY, USA) with Dulbecco's modified Eagle's medium (DMEM-Sigma Chemical Co, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS), incubated at 37 °C and 5% CO 2 , for 24 h to adhere.…”