Storage Cells - Oil and Protein Bodies

Gallardo-Guerrero, Karine; Jolivet, Pascale; Vernoud, Vanessa; Canonge, Michel; Larré, Colette; Chardot, Thierry

doi:10.1201/b20316-24

Cited by 3 publications

(5 citation statements)

References 142 publications

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“…Oilseeds store lipids in oil bodies, which are relatively simple organelles, consisting of a matrix of TAG coated with a phospholipid monolayer embedded with oleosins ( Siloto et al, 2006 ). Oil bodies are formed via an endoplasmic reticulum (ER)-budding process during seed development ( Sarmiento et al, 1997 ; Hsieh and Huang, 2004 ; Wu et al, 2010 ) and they are detected as early as the heart stage of embryo development ( Siloto et al, 2006 ; Gallardo et al, 2016 ). Besides seeds, oil bodies are found in many different tissues and organs ( Siloto et al, 2006 ; Song et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Expression Profiling, and Functional Validation of Oleosin Gene Family in Carthamus tinctorius L.

Chi

et al. 2018

Front. Plant Sci.

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Carthamus tinctorius L., commonly known as safflower, is an important oilseed crop containing oil bodies. Oil bodies are intracellular organelles in plant cells for storing triacylglycerols (TAGs) and sterol esters. Oleosins are the most important surface proteins of the oil bodies. We predicted and retrieved the sequences of eight putative C. tinctorius oleosin (Ctoleosin) genes from the genome database of safflower. The bioinformatics analyses revealed the size of their open reading frames ranging from 414 to 675 bp, encoding 137 to 224 aa polypeptides with predicted molecular weights of 14.812 to 22.155 kDa, all containing the typical “proline knot” motif. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) determined the spatiotemporal expression pattern of Ctoleosin genes, which gradually increased and peaked during flowering and seed ripening, and decreased thereafter. To validate their role in plant development, we transformed and overexpressed these eight putative Ctoleosin genes in Arabidopsis. Overexpressing Ctoleosins did not affect leaf size, although silique length was altered. Arabidopsis transformed with Ctoleosin3, 4, and 5 grew longer siliques than did the wild-type plants, without altering seed quantity. The 100-grain weight of the transgenic Arabidopsis seeds was slightly more than that of the wild-type seeds. The seed germination rates of the plants overexpressing Ctoleosin4 and 6 were slightly lower as compared with that of the wild-type Arabidopsis, whereas that in the other transgenic lines were higher than that in the wild-type plants. The overexpression of Ctoleosin genes elevated the oil content in the seeds of transgenic Arabidopsis. Our findings not only provide an approach for increasing the oil content, but also for elucidating the intricate mechanisms of oil body synthesis.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification, Expression Profiling, and Functional Validation of Oleosin Gene Family in Carthamus tinctorius L.

Chi

et al. 2018

Front. Plant Sci.

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“…The oil body can be detected as early as the heart stage in Brassica napus (He and Wu 2009 ). The different oleosin isoforms, caleosin and steroleosin accumulate sequentially throughout seed development (Gallardo et al 2016 ). Fatty acid accumulation starts from 8 days after pollination, corresponding to the heart stage in M. truncatula (Wang et al 2012 ).…”

Section: Oil Bodies In Legumesmentioning

confidence: 99%

“…Fatty acid accumulation starts from 8 days after pollination, corresponding to the heart stage in M. truncatula (Wang et al 2012 ). While this review focuses on seed oil bodies, oil bodies can be present in many different tissues and organs (Gallardo et al 2016 ).…”

Section: Oil Bodies In Legumesmentioning

confidence: 99%

“…In Chlamydomonas when MLDP synthesis is inhibited, oil body size increases (Goold et al 2015 ). In addition to oleosins, there are a number of other proteins associated with plant oil bodies (Gallardo et al 2016 ). It seems likely that this includes peripheral proteins associated with the surface structures of oil bodies for oil body mobilization during germination (Feussner et al 2001 ; Jolivet et al 2013 ).…”

Section: Oil Body Biogenesismentioning

confidence: 99%

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Oil body biogenesis and biotechnology in legume seeds

2017

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The seeds of many legume species including soybean, Pongamia pinnata and the model legume Medicago truncatula store considerable oil, apart from protein, in their cotyledons. However, as a group, legume storage strategies are quite variable and provide opportunities for better understanding of carbon partitioning into different storage products. Legumes with their ability to fix nitrogen can also increase the sustainability of agricultural systems. This review integrates the cell biology, biochemistry and molecular biology of oil body biogenesis before considering biotechnology strategies to enhance oil body biosynthesis. Cellular aspects of packaging triacylglycerol (TAG) into oil bodies are emphasized. Enhancing seed oil content has successfully focused on the up-regulation of the TAG biosynthesis pathways using overexpression of enzymes such as diacylglycerol acyltransferase1 and transcription factors such as WRINKLE1 and LEAFY COTYLEDON1. While these strategies are central, decreasing carbon flow into other storage products and maximizing the packaging of oil bodies into the cytoplasm are other strategies that need further examination. Overall there is much potential for integrating carbon partitioning, up-regulation of fatty acid and TAG synthesis and oil body packaging, for enhancing oil levels. In addition to the potential for integrated strategies to improving oil yields, the capacity to modify fatty acid composition and use of oil bodies as platforms for the production of recombinant proteins in seed of transgenic legumes provide other opportunities for legume biotechnology.

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“…Pumpkin storage proteins are 2S albumins and 11S globulins (cucurbitin) localized in the protein bodies (Gallardo et al, 2016;Hegedus et al, 2015;Shimada et al, 2017). Alpha amylase inhibitors have potential roles in controlling blood sugar levels.…”

mentioning

confidence: 99%

Role of Antihypertensive Peptides from Plant (sesame, almond and pumpkin) Seed Storage Protein

2017

Agriculture and Life Sciences Resaerch Institute

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Plant proteins are important functional ingredients in many processed food products. Particularly globular proteins from various sources play an important role in many food products, due their contribution to food texture (McClements and Gumus, 2016). The seed storage proteins have been traditionally classified through the sequential extraction of crushed and defatted seeds by a series of aqueous and non-aqueous solvents (Marcone et al., 2002). The fraction extracted with water is defined as albumins, the fraction with dilute salt as globulins, the fraction with ethanol as prolamines, and the fraction with acid or alkali as glutelins. In cereal grains the main storage proteins are usually the alcohol soluble prolamines, whereas, in non-cereal grains the more nutritiously balanced salt soluble

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Storage Cells - Oil and Protein Bodies

Cited by 3 publications

References 142 publications

Genome-Wide Identification, Expression Profiling, and Functional Validation of Oleosin Gene Family in Carthamus tinctorius L.

Genome-Wide Identification, Expression Profiling, and Functional Validation of Oleosin Gene Family in Carthamus tinctorius L.

Oil body biogenesis and biotechnology in legume seeds

Role of Antihypertensive Peptides from Plant (sesame, almond and pumpkin) Seed Storage Protein

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