Stimuli-responsive interfaces and systems for the control of protein–surface and cell–surface interactions

Cole, Martin A.; Voelcker, Nicolas H.; Thissen, Helmut; Griesser, Hans J.

doi:10.1016/j.biomaterials.2008.12.026

Cited by 424 publications

(353 citation statements)

References 244 publications

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“…Reversible control of cell adhesion has so far mainly been limited to switching adhesion by use of thermoresponsive materials, such as poly( Nisopropylacrylamide), [ 10,11 ] which are ill-suited for local and rapid switching at high spatial resolution. In contrast, light-induced switching of cell adhesion could prove to be powerful in controlling cell adhesion at high spatial and temporal resolution.…”

Section: Doi: 101002/adma201504394mentioning

confidence: 99%

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

et al. 2015

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Section: Doi: 101002/adma201504394mentioning

confidence: 99%

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

et al. 2015

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“…1-5 Many of the reported polymer brushes so far have been developed for use as antifouling surfaces, due to the ability to tune polymer chemistry to resist short-term protein adsorption and subsequent cell adhesion. [6][7][8][9] Thermo-responsive polymer brushes have been considered as functional substrate modifiers as their surface wettability can be easily adjusted by changing temperature, enabling application in controlled cell adhesion and detachment. 10,11 The most widely studied thermoresponsive polymer is poly(N-isopropylacrylamide) (PNiPAm), which exhibits a lower critical solution temperature (LCST) phase transition in water at 32 ⁰C.…”

Section: Introductionmentioning

confidence: 99%

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

et al. 2017

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We report the synthesis of thermo-responsive polymer brushes with Upper Critical Solution Temperature (UCST)-type behaviour on glass to provide a new means to control cell attachment. Thermoresponsive poly(N-acryloyl glycinamide)-statpoly(N-phenylacrylamide) (PNAGAm-PNPhAm) brushes with three different monomer ratios were synthesized to give tunable phase transition temperatures (Tp) in solution. Surface energies of surface-grafted brushes of these polymers at 25, 32, 37 and 50 C were calculated from contact angle measurements and atomic force microscopy (AFM) studies confirmed that these polymers were highly extended at temperatures close to Tp in physiologically-relevant media. Importantly, NIH-3T3 cells were attached on the collapsed PNAGAm-PNPhAm brush surface at 30 C after 20 h incubation, while release of cells from the extended brushes was observed within 2 h after the culture temperature was switched to 37 C. Furthermore, the changes in cell attachment followed changes in the Lewis base component of surface energy. The results indicate that, in contrast to the established paradigm of enhanced cell attachment to surfaces where polymers are above a Lower Critical Solution Temperature (LCST), these novel substrates enable detachment of cells from surfaces at temperatures above a UCST. In turn these responsive materials open new avenues for the use of polymer-modified surfaces to control cell attachment for applications in cell manufacture and regenerative medicine.

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“…To the best of our knowledge, this is the first study of bicompartmental nanofibers composed of a physically-crosslinked, thermoresponsive compartment, along with a chemically-crosslinked compartment showing a thermally-triggered mechanical actuation and fast response to external stimuli because of high surface area-tovolume ratios, which make them unique choices for the development of smart drug delivery systems for stimuli-triggered, controlled drug release. 2,5 Furthermore, when bovine serum albumin (BSA) and dexamethasone 21-phosphate (DMP) were separately loaded into each compartment, the bicompartmental nanofibers with mechanical actuation showed fully decoupled, controlled release profiles in response to temperature. This new class of multicompartmental nanofibers could be useful as advanced nanofiber scaffolds with two or more drugs that can be released under different release kinetics in response to environmental stimuli.…”

mentioning

confidence: 99%

Fabrication and characterization of anisotropic nanofiber scaffolds for advanced drug delivery systems

Jalani

Jung

Lee

et al. 2014

IJN

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Stimuli-responsive, polymer-based nanostructures with anisotropic compartments are of great interest as advanced materials because they are capable of switching their shape via environmentally-triggered conformational changes, while maintaining discrete compartments. In this study, a new class of stimuli-responsive, anisotropic nanofiber scaffolds with physically and chemically distinct compartments was prepared via electrohydrodynamic cojetting with side-by-side needle geometry. These nanofibers have a thermally responsive, physically-crosslinked compartment, and a chemically-crosslinked compartment at the nanoscale. The thermally responsive compartment is composed of physically crosslinkable poly(N-isopropylacrylamide) poly(NIPAM) copolymers, and poly(NIPAM-co-stearyl acrylate) poly(NIPAM-co-SA), while the thermally-unresponsive compartment is composed of polyethylene glycol dimethacrylates. The two distinct compartments were physically crosslinked by the hydrophobic interaction of the stearyl chains of poly(NIPAM-co-SA) or chemically stabilized via ultraviolet irradiation, and were swollen in physiologically relevant buffers due to their hydrophilic polymer networks. Bicompartmental nanofibers with the physically-crosslinked network of the poly(NIPAM-co-SA) compartment showed a thermally-triggered shape change due to thermally-induced aggregation of poly(NIPAM-co-SA). Furthermore, when bovine serum albumin and dexamethasone phosphate were separately loaded into each compartment, the bicompartmental nanofibers with anisotropic actuation exhibited decoupled, controlled release profiles of both drugs in response to a temperature. A new class of multicompartmental nanofibers could be useful for advanced nanofiber scaffolds with two or more drugs released with different kinetics in response to environmental stimuli.

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Stimuli-responsive interfaces and systems for the control of protein–surface and cell–surface interactions

Cited by 424 publications

References 244 publications

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

Rapid Reversible Photoswitching of Integrin‐Mediated Adhesion at the Single‐Cell Level

Upper critical solution temperature thermo-responsive polymer brushes and a mechanism for controlled cell attachment

Fabrication and characterization of anisotropic nanofiber scaffolds for advanced drug delivery systems

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