Stimulatory Effect of Autologous Adipose Tissue-Derived Stromal Cells in an Atelocollagen Matrix on Wound Healing in Diabetic db/db Mice

Abstract

We aimed to evaluate the effectiveness of the application of an atelocollagen matrix containing autologous adipose tissue-derived stromal cells (ASCs) on wound healing in diabetic (db/db) mice. Cultured ASCs from db/db mice and from db/+ mice secreted identical amounts of growth factors, cytokines, and type I collagen. ASCs from db/db mice proliferated at the same rate as those from db/+ mice. When DiI-labeled ASCs were applied to full-thickness round skin wounds on the backs of diabetic db/db mice, histological observation at 2 weeks showed that red fluorescent-labeled tissues were formed in the epidermis, dermis, and capillaries. Twelve db/db mice were treated with either matrix alone or matrix containing ASCs and then sacrificed at 1 or 2 weeks. A histological examination demonstrated significantly advanced granulation tissue formation, capillary formation, and epithelialization in those wounds treated with atelocollagen matrix containing ASCs, compared with wounds treated with matrix alone.

Fibroblast growth factor 2-functionalized collagen matrices for skeletal muscle tissue engineering

“…Hence, collagen has been used commercially in various medical applications and wound dressings since the 1970s. Particularly, collagen is clinically wellestablished and FDA-approved matrices for wound healing to treat burns and chronic wounds (Still et al 2003;Harding et al 2002;Nambu et al 2011). The formulations of collagen used in medical application vary such as gel, film, sponge and powder.…”

Effective Wound Healing in Streptozotocin-Induced Diabetic Rats by Adipose-Derived Stromal Cell Transplantation in Plasma-Gel Containing Fragmin/Protamine Microparticles

“…Because the infiltrated neutrophils and macrophages had a significant effect on the granulation tissue formation of repair-impaired wounds in db/db mice, 30 it is likely that sufficient infiltrated neutrophils and macrophages accumulate in STZ(j)-rats but not in STZ-rats. Thus, the STZ(j)-rats might have a sufficient amount of GFs in the wound and wound f luid to permit a better healing, and therefore, little increase in wound repair may result from the application of the STZ(j)-rats.…”

“…Normal wound healing proceeds in 3 overlapping phases, namely, inf lammation, proliferation, and remodeling. 30 The sequential process requires the interaction of cells in the dermis and epidermis and the activity of chemical mediators released from cells. An absence of the cellular and molecular signals required for normal wound repair processes may contribute to poor healing of some wounds, such as diabetic wound.…”

“…An absence of the cellular and molecular signals required for normal wound repair processes, such as inf lammation, angiogenesis, contraction, granulation tissue formation, epithelialization, deposition of extracellular matrix, and remodeling, may contribute to poor healing of some wounds, such as diabetic wound. 30 In the present study, we applied STZ to make a diabetic rat model, and evaluated the efficacy of IR-ASCs + high IRP (6%)-DMEM gel + F/P MPs in repairing healing-impaired wounds in this model of diabetes.…”

“…Normal wound healing proceeds in 3 overlapping phases, namely, inf lammation, proliferation (including granulation tissue and matrix formation), and remodeling. 30 The sequential process requires the interaction of cells in the dermis and epidermis with the activity of chemical mediators released from inf lammatory cells, fibroblasts, and keratinocytes. An absence of the cellular and molecular signals required for normal wound repair processes, such as inf lammation, angiogenesis, contraction, granulation tissue formation, epithelialization, deposition of extracellular matrix, and remodeling, may contribute to poor healing of some wounds, such as diabetic wound.…”