Stimulation of the dentine–pulp complex of rat incisor teeth by transforming growth factor-β isoforms 1–3 in vitro

Sloan, Alastair James; Smith, Anthony J.

doi:10.1016/s0003-9969(98)00106-x

Cited by 193 publications

(150 citation statements)

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“…However, unlike the wild type mice, the pulpal cells of dTGF-␤1 mice displayed patchy and ocular staining for collagen I and III, indicating the differentiation of dental pulp into odontoblast-like cells as a result of secreted TGF-␤1 protein. Differentiation of dental pulp into collagen producing odontoblast-like cells has been suggested in in vitro and ex vivo culture systems by addition of exogenous growth factors, either alone or in combination, to understand the development of odontoblasts (10). Increased levels of collagen I and III and their abnormal accumulation in the pulp seen in dspp/TGF-␤1 transgenic mice are reminiscent of similar changes observed in dentinogenesis imperfecta and dentin dysplasia (27,28).…”

Section: Discussionmentioning

confidence: 99%

“…Odontoblasts produce DECM from embryonic day 16 and subsequently mineralize in an orderly manner. TGF-␤1 has been shown earlier to have mitogenic effects in tooth explant cultures (10) and to induce secretion of DECM components. Although it has been suggested that TGF-␤1 plays a crucial role in dental tissue repair processes by the induction of reactionary (11) and reparative dentinogenesis (12), the precise in vivo functions associated with its continued expression are not clearly understood.…”

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confidence: 95%

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Reduced Expression of Dentin Sialophosphoprotein Is Associated with Dysplastic Dentin in Mice Overexpressing Transforming Growth Factor-β1 in Teeth

Thyagarajan

Sreenath

Cho

et al. 2001

Journal of Biological Chemistry

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Transforming growth factor (TGF)-␤1 is expressed in developing tooth from the initiation stage through adulthood. Odontoblast-specific expression of TGF-␤1 in the tooth continues throughout life; however, the precise biological functions of this growth factor in the odontoblasts are not clearly understood. Herein, we describe the generation of transgenic mice that overexpress active TGF-␤1 predominantly in the odontoblasts. Teeth of these mice show a significant reduction in the tooth mineralization, defective dentin formation, and a relatively high branching of dentinal tubules. Dentin extracellular matrix components such as type I and III collagens are increased and deposited abnormally in the dental pulp, similar to the hereditary human tooth disorders such as dentin dysplasia and dentinogenesis imperfecta. Calcium, one of the crucial inorganic components of mineralization, is also apparently increased in the transgenic mouse teeth. Most importantly, the expression of dentin sialophosphoprotein (dspp), a candidate gene implicated in dentinogenesis imperfecta II (MIM 125420), is significantly down-regulated in the transgenic teeth. Our results provide in vivo evidence suggesting that TGF-␤1 mediated expression of dspp is crucial for dentin mineralization. These findings also provide for the first time a direct experimental evidence indicating that decreased dspp gene expression along with the other cellular changes in odontoblasts may result in human hereditary dental disorders like dentinogenesis imperfecta II (MIM 125420) and dentin dysplasia (MIM 125400 and 125420).Mammalian development is a complex and highly orchestrated process that involves intricate cross-talk between growth factors and other regulatory molecules. These molecules interact with each other to induce specific molecular and cellular changes leading to organogenesis. Interactions between epithelium and mesenchyme are particularly crucial during the initiation of development of key organs such as teeth, skin, hair, mammary gland, and prostate (1). Tooth development is initiated by epithelial-mesenchymal interactions in the first branchial arch, and several transcription factors and growth factors are known to be expressed by dentin extracellular matrix (DECM)-producing 1 odontoblasts and enamel-producing ameloblasts during tooth development (2-5). Transforming growth factor-␤1 (TGF-␤1), a prototype of the TGF-␤ superfamily, is a multi-functional growth factor expressed in a wide variety of developing tissues from the early stages. The regulation of cell proliferation, differentiation, embryonic development, and apoptosis by TGF-␤1 is well established (6 -8). During mouse tooth development, TGF-␤1 is expressed initially in the oral epithelium at embryonic day 13, and later its expression extends into the mesenchymal compartment and then gets restricted to the ectomesenchymal layer (odontoblasts). The odontoblast-restricted expression of TGF-␤1 persists throughout life in the mice (9). Odontoblasts produce DECM from embryonic day 16 and subseq...

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Section: Discussionmentioning

confidence: 99%

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confidence: 95%

Reduced Expression of Dentin Sialophosphoprotein Is Associated with Dysplastic Dentin in Mice Overexpressing Transforming Growth Factor-β1 in Teeth

Thyagarajan

Sreenath

Cho

et al. 2001

Journal of Biological Chemistry

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“…Smooth muscle α-actin is a microfilament (5 to 8 nm in diameter) that composes the cytoskeleton of all mammalian smooth muscle cells, myoepithelial cells, and is particularly abundant in smooth muscle cells of vessel walls 17 , having important contractile function in these cells and being considered a marker of the myofibroblastic phenotype 18,19 . It is also present in conjunctive cells, such as fibroblasts 1,20 and myofibroblasts 21 . Ultrastructurally, they have well-developed rough endoplasmic reticulum and Golgi complex, pinocytosis vesicles, gap junctions, and organized arrangement of actin myofilament bundles focallylocated along the cell axis (stress fibers) or binding to extracellular fibronectin domains through specialized membrane proteins (integrins), characterizing membrane specializations called fibronexes 10,21 .…”

Section: Discussionmentioning

confidence: 99%

Avaliação da eficácia de três marcadores imunoistoquímicos envolvidos nos diferentes tempos da cicatrização da ferida cirúrgica

Filho

Ariede

et al. 2017

Rev. Col. Bras. Cir.

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“…TGF-β2 has been shown to be present in mature dentin; therefore, it seems that TGF-β2 also stimulates odontoblast differentiation in maturing dentin to increase the dentin mineral apposition rate. 15 An in vitro study by Sloan and Smith 41 revealed that TGF-β1 and TGF-β3 increase the predentin thickness of the cultured tooth in the region immediately adjacent to agarose beads containing those growth factors. The localized increase in the predentin thickness reflects reactionary dentinogenic effect.…”

Section: Discussionmentioning

confidence: 99%

“…47 Several studies reported that TGF-β1 and TGF-β3 stimulate proliferation of the cells of the subodontoblast layer and formation of odontoblast-like cells. 13,41,48 In addition, TGF-β1 induces type I collagen production by the odontoblastic/subodontoblastic pulp cells. 49 Huojia et al 50 reported that TGF-β3 induces ectopic mineralization through upregulation of osteocalcin and type I collagen expression in the dental pulp cells and may regulate the differentiation of the dental pulp stem cells to odontoblasts.…”

Section: Discussionmentioning

confidence: 99%

The role of transforming growth factor beta in tertiary dentinogenesis

Haniastuti

Nunez

Djais

2008

Dent. J. (Majalah Kedokteran Gigi)

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The most visible repair response to pulp injury is the deposition of a tertiary dentin matrix over the dentinal tubules of the primary or secondary dentin. Tertiary dentin is distinguished as reactionary and reparative dentin, depending on the severity of the initiating response and the conditions under which the newly deposited dentin matrix was elaborated. Transforming growth factor beta (TGF-b)

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Stimulation of the dentine–pulp complex of rat incisor teeth by transforming growth factor-β isoforms 1–3 in vitro

Cited by 193 publications

References 23 publications

Reduced Expression of Dentin Sialophosphoprotein Is Associated with Dysplastic Dentin in Mice Overexpressing Transforming Growth Factor-β1 in Teeth

Reduced Expression of Dentin Sialophosphoprotein Is Associated with Dysplastic Dentin in Mice Overexpressing Transforming Growth Factor-β1 in Teeth

Avaliação da eficácia de três marcadores imunoistoquímicos envolvidos nos diferentes tempos da cicatrização da ferida cirúrgica

The role of transforming growth factor beta in tertiary dentinogenesis

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