Stimulation of synaptosomal Na+,K+-ATPase by ethanol: possible involvement of an isozyme-specific inhibitor of Na+,K+-ATPase

Foley, Timothy D.; Rhoads, Dennis E.

doi:10.1016/0006-8993(94)90386-7

Cited by 14 publications

(17 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Alcohol has been shown to modulate Na + /K + pumping in preparations from rodent brains (Foley and Rhoads, 1994; Ledig et al, 1985; Syapin et al, 1985). It would be interesting to investigate if alcohol inhibits Na + /K + pumping in cerebellar Purkinje neurons, and whether this is a factor in the motor dysfunction concordant with inebriation.…”

Section: Discussionmentioning

confidence: 99%

Intracellular calcium dynamics permit a Purkinje neuron model to perform toggle and gain computations upon its inputs

Forrest

2014

Front. Comput. Neurosci.

View full text Add to dashboard Cite

Without synaptic input, Purkinje neurons can spontaneously fire in a repeating trimodal pattern that consists of tonic spiking, bursting and quiescence. Climbing fiber input (CF) switches Purkinje neurons out of the trimodal firing pattern and toggles them between a tonic firing and a quiescent state, while setting the gain of their response to Parallel Fiber (PF) input. The basis to this transition is unclear. We investigate it using a biophysical Purkinje cell model under conditions of CF and PF input. The model can replicate these toggle and gain functions, dependent upon a novel account of intracellular calcium dynamics that we hypothesize to be applicable in real Purkinje cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Intracellular calcium dynamics permit a Purkinje neuron model to perform toggle and gain computations upon its inputs

Forrest

2014

Front. Comput. Neurosci.

View full text Add to dashboard Cite

show abstract

“…This pattern of acute inhibition of the enzyme with an apparently compensatory increase in activity during chronic alcohol exposure can be modeled in cultured cells (Rabin 1988; Rabin and Acara 1993) but was not observed in all animal models studied (Goldstein and Israel 1972;Akera et al 1973;Rangaraj and Kalant 1984;Leventhal and Tabakoff 1980;Aloia et al 1985;Nhamburo et al 1987;Foster et al 1989;Bertoni and Sprenkle 1994). Results appear to be sensitive to animal strain and route and duration of alcohol exposure (discussed in Rabin 1988;Chen et al 1997), but also to the in vitro conditions used in preparing the membranes (Swann 1990;Foley and Rhoads 1994) and assaying Na,KATPase activity (Israel et al 1970;Foster et al 1989). This is underscored by the observation that ethanol in vitro may stimulate or inhibit Na,KATPase depending on the ionic environment (Foster et al 1989).…”

Section: Introductionmentioning

confidence: 86%

“…1). Activities of the isozymes were based on the differential sensitivity of the enzyme to ouabain (Brodsky 1990;Brodsky and Guidotti 1990) as described previously (Foley and Rhoads 1994;Wang et al 1998). Because of their greater sensitivity to inhibition by ouabain, the combined activities of the a2 and a3 isozymes (a2/3) were operationally defined as the difference between ATP hydrolysis in the absence of ouabain and in the presence of 10 lM ouabain.…”

Section: Enzyme Assaymentioning

confidence: 99%

“…In addition to differences in cell, tissue, and developmental distribution, isozymes of Na,K-ATPase also differ in sensitivity to ouabain and regulation (Orlowski and Lingrel 1988;Sweadner 1989Sweadner , 1992. The form(s) of Na,K-ATPase with higher sensitivity to ouabain (a2 and/or a3 isozymes) may be differentially sensitive to ethanol (Marks et al 1984;Nhamburo et al 1987;Foley and Rhoads 1994).…”

Section: Introductionmentioning

confidence: 97%

“…This is underscored by the observation that ethanol in vitro may stimulate or inhibit Na,KATPase depending on the ionic environment (Foster et al 1989). While adolescent brain Na,K-ATPase may respond differently to acute effects of ethanol (Foley and Rhoads 1992), distinction among the specific isozymes of Na,K-ATPase may also be important (Foley and Rhoads 1994).…”

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Altered Pattern of Na,K-ATPase Activity and mRNA During Chronic Alcohol Consumption by Juvenile and Adolescent Rats

2008

Self Cite

View full text Add to dashboard Cite

The effect of chronic ethanol consumption on cerebral cortical activity of Na,K-ATPase was determined in Long-Evans (LE) rats fed an ethanol-containing diet beginning at different stages of development. Na,K-ATPase activity was operationally resolved into alpha1 and alpha2/3 isozyme activities. There was no significant difference in Na,K-ATPase activities before and after alcohol consumption in the preparations from adult rats. However, for rats beginning alcohol consumption as adolescents, the alpha2/3 activity was significantly elevated following chronic alcohol consumption. Both LE and Sprague-Dawley rats showed this same selective increase in cortical alpha2/3 activity when rats began alcohol consumption as juveniles. The shift in cortical alpha2/3 activity was not observed in cerebellum or subcortical forebrain and was reversible when rats were fed ethanol throughout the normal adolescent period and then withdrawn and tested 2 weeks later (during the adult period). Levels of isoform-specific mRNA were determined in preparations of cerebral cortices of rats showing elevated alpha2/3 isozyme activities. In these preparations, isoform specific alpha2 and alpha3 mRNA was significantly elevated. There was no effect of ethanol feeding on cortical alpha1 mRNA. These findings indicate that the longer term effects of ethanol on the developing brain include elevated Na,K-ATPase activity and a mechanism that is pre-translational and isoform specific.

show abstract

Attenuation by methyl mercury and mercuric sulfide of pentobarbital induced hypnotic tolerance in mice through inhibition of ATPase activities and nitric oxide production in cerebral cortex

Chuu

Huang

et al. 2007

Arch Toxicol

View full text Add to dashboard Cite

This study is aimed at exploring the possible mechanism of hypnosis-enhancing effect of HgS or cinnabar (a traditional Chinese medicine containing more than 95% HgS) in mice treated with pentobarbital. We also examined whether the effect of HgS is different from that of the well-known methyl mercury (MeHg). After a short period (7 days) of oral administration to mice, a nontoxic dose (0.1 g/kg) of HgS not only significantly enhanced pentobarbital-induced hypnosis but also attenuated tolerance induction; while a higher dose (1 g/kg) of HgS or cinnabar exerted an almost irreversible enhancing effect on pentobarbital-hypnosis similar to that of MeHg (2 mg/kg) tested, which was still effective even after 10 or 35 days cessation of administration. To study comparatively the effects of different mercury forms from oral administration of MeHg and HgS on membrane ATPase activities of experimental mice, analysis of the Hg content in the cerebral cortex revealed that correlated with the decrease of Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities. Furthermore, NO levels of blood but not that of cerebral cortex were also decreased by mercuric compounds. Although pentobarbital alone enhanced cytochrome p450-2C9 in time dependent manner, all of mercurial compounds tested had no such effect. All of these findings indicated that the mercurial compounds including cinnabar, HgS and MeHg exert a long-lasting enhancing hypnotic activity without affecting pentobarbital metabolism, which provides evidence-based sedative effect of cinnabar used in Chinese traditional medicine for more than 2,000 years. The nontoxic HgS dosing (0.1 g/kg/day) for consecutive 7 days is perhaps useful for delaying or preventing pentobarbital-tolerance.

show abstract

Stimulation of synaptosomal Na+,K+-ATPase by ethanol: possible involvement of an isozyme-specific inhibitor of Na+,K+-ATPase

Cited by 14 publications

References 21 publications

Intracellular calcium dynamics permit a Purkinje neuron model to perform toggle and gain computations upon its inputs

Intracellular calcium dynamics permit a Purkinje neuron model to perform toggle and gain computations upon its inputs

Altered Pattern of Na,K-ATPase Activity and mRNA During Chronic Alcohol Consumption by Juvenile and Adolescent Rats

Attenuation by methyl mercury and mercuric sulfide of pentobarbital induced hypnotic tolerance in mice through inhibition of ATPase activities and nitric oxide production in cerebral cortex

Contact Info

Product

Resources

About