Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Jung, Gi-Dong; Yang, Jeong‐Yeh; Song, Eun-Sup; Park, Jin-Woo

doi:10.1038/emm.2001.23

Cited by 51 publications

(38 citation statements)

References 24 publications

(19 reference statements)

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“…Cells were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 μg/ml amphotericin B at 37 o C in a humidified 95% air/5% CO2 atmosphere as described previously (Jung et al, 2001). XH was obtained from Alexis Biochemicals (Lausen, Switzerland), and α-MSH, IBMX, and forskolin were obtained from Sigma (St. Louis, MO).…”

Section: Cells and Materialsmentioning

confidence: 99%

Effect of xanthohumol on melanogenesis in B16 melanoma cells

et al. 2008

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Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited α-melanocyte stimulating hormone-or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia-associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.

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Section: Cells and Materialsmentioning

confidence: 99%

Effect of xanthohumol on melanogenesis in B16 melanoma cells

et al. 2008

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“…The B16F10 murine melanoma cell line was obtained from the Tumor Researching Department of The Third Affiliated Hospital of Harbin Medical University, Harbin, China. Cells were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, 0.1 mg/ml streptomycin, and 0.25 μg/ml amphotericin B at 37˚C in a humidified 95% air/5% CO 2 atmosphere as described previously (11). Drug treatment began 24 h after seeding, and cells were harvested after indicated concertations and times of incubation.…”

Section: Introductionmentioning

confidence: 99%

Baicalein inhibits melanogenesis through activation of the ERK signaling pathway

2010

Int J Mol Med

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Abstract. Baicalein is one of the major flavonoids in Scutellaria baicalensis. However, the effects of baicalein on melanogenesis are unknown. The objective of this study was to evaluate the depigmenting capacity of baicalein and to elucidate its mechanism of action. B16F10 mouse melanoma cells were used to examine the effect of baicalein on melanogenesis by measurement of melanin content and tyrosinase activity after treatment. To ascertain the baicalein activity, the effect on two protein kinases, ERK and Akt and downstream microphthalmia-associated transcription factor (MITF) were examined by Western blotting and RT-PCR. Baicalein significantly inhibited melanin synthesis in a concentration-dependent manner without cytotoxicity. Tyrosinase activity was also reduced. Baicalein decreased MITF and tyrosinase levels but did not decrease MITF mRNA. Western blotting showed that baicalein induced ERK activation. Using the specific ERK phosphorylation inhibitor, PD98059, we blocked the hypopigmentation effect, and also abrogated the baicalein-mediated activation of ERK. However, baicalein did not induce Akt activation. These results suggest that the ERK pathway is involved in the melanogenic signaling cascade, and that ERK activation by baicalein reduces melanin synthesis via MITF downregulation and is subsequent to the inhibition of tyrosinase synthesis.

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“…Furthermore, it has been shown that extracts from some herbs, including kava rhizome extract (43), glycyrrhizin (44), lotus flower (45), cubebin (46), quercetin (47) and naringenin (17) increase melanogenesis in B16 melanoma cells and human melanocytes, thereby indicating that natural resources should be extensively screened for the development of gray hair prevention agents or therapeutic drugs to induce repigmentation in the skin of vitiligo patients.…”

Section: Discussionmentioning

confidence: 99%

Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression

2011

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Abstract. Melanin synthesis is stimulated by various effectors, including α-melanocyte stimulating hormone (α-MSH), cyclic AMP (cAMP)-elevating agents (forskolin, isobutylmethylxantine, glycyrrhizin) and ultraviolet light. Our investigation focused on the identification of the melanogenic efficacy of mangosteen (Garcinia mangostana) leaf extract with regard to its effects on melanogenesis in B16F1 melanoma cells, since it has been known to possess strong anti-oxidant activities. The mangosteen leaf extract was found to stimulate melanin synthesis and tyrosinase activity in a dose-dependent manner without any significant effects on cell proliferation. Cytotoxicity of the extract was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; the highest concentration of the extract that did not affect cell viability was 32 µg/ml. Formation of melanin from cultured B16F1 melanoma induced by extract treatment was estimated using spectrophotometry. In order to clarify the subsequent mechanism of tyrosinase activation by the extract, the levels of tyrosinase expression in B16F1 melanoma were examined using an intracellular tyrosinase assay and tyrosinase zymography. Up-regulation of intracellular tyrosinase expression seemed to correlate with an increase in microphtalmia-associated transcription factor (MITF) protein levels since MITF is the key factor for genes involved in melanogenesis. Both of the results showed that tyrosinase activity was markedly enhanced from extract-treated cells. The overall results suggest that mangosteen leaf extract may be a promising candidate for the treatment of hypopigmentation disorder and useful for selftanning cosmetic products.

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Stimulation of melanogenesis by glycyrrhizin in B16 melanoma cells

Cited by 51 publications

References 24 publications

Effect of xanthohumol on melanogenesis in B16 melanoma cells

Effect of xanthohumol on melanogenesis in B16 melanoma cells

Baicalein inhibits melanogenesis through activation of the ERK signaling pathway

Mangosteen leaf extract increases melanogenesis in B16F1 melanoma cells by stimulating tyrosinase activity in vitro and by up-regulating tyrosinase gene expression

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