Stimulation by D-glucose of the direct conversion of arginine to citrulline in enterocytes isolated from pig jejunum

An Improved Assay for Measurement of Nitric Oxide Synthase Activity in Biological Tissues

“…It is important to define the L-NAME-inhibitable counts to fully characterize NOS-derived activity. It has been described that nephritic glomeruli can switch L-arginine metabolism from NOS to arginase due to changes in substrate concentration (19) or that glucose can stimulate direct conversion of L-arginine into L-citrulline in pig jejunum enterocytes (20). Therefore, it is important that NOS activity be confirmed by enzyme blockade with a NOS specific inhibitor such as L-NAME.…”

Fuel selection in intestinal cells

“…Glucose oxidation corresponds to C02 production (in the pyruvate dehydrogenase complex and tricarboxylic acid cycle). The pentose phosphate pathway contributes weakly to C 0 2 generation (Mallet et al 1986~;Blachier et al 1991b). Thus, the generation of pyruvate and its oxidation determines the rate of glucose oxidation.…”

Glucose, Galactose, and Glutamine Metabolism in Pig Isolated Enterocytes during Development

“…The isolated cells were washed twice and resuspended in the same bicarbonate buffer medium (pH 7.4) without D m and containing 1.3 mM CaCI,, 2 mM MgCI2, and 1% fatty acid-free albumin (incubation buffer). Postweaned pigs were anesthetized with halothane and enterocytes from the jejunum were isolated as previously reported (27). To assess the origin of the cells removed, histologic examinations of the jejunum and ileum from 0-and 2-d-old animals were performed before and after EDTA treatment.…”