Stiffness regulates the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells via the <scp>WNT</scp> signalling pathway

Liu, Nanxin; Zhou, Mi; Zhang, Qi; Zhang, Tao; Tian, Taoran; Ma, Qing; Xue, Changyue; Lin, Shiyu; Cai, Xiaoxiao

doi:10.1111/cpr.12435

Cited by 45 publications

(33 citation statements)

References 43 publications

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“…Stem cells differentiate into muscle cells on soft substrates and osteoblasts on harder substrates [86,87]. Another study supported this finding, and stem cell on soft materials when stiffness is less than 0.05 kPa could promote neural differentiation effectively, while hard stiffness materials (>40 kPa) promoted osteogenic differentiation effectively [88,89], which could be related to the Wnt signal pathway [90]. However, there is no agreement on the optimal stiffness for stem cells to differentiate into neurons, muscle cells, cartilage cells, and osteoblasts [86,91].…”

Section: Stiffness Effectsmentioning

confidence: 74%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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It is well known that stem cells reside within tissue engineering functional microenvironments that physically localize them and direct their stem cell fate. Recent efforts in the development of more complex and engineered scaffold technologies, together with new understanding of stem cell behavior in vitro, have provided a new impetus to study regulation and directing stem cell fate. A variety of tissue engineering technologies have been developed to regulate the fate of stem cells. Traditional methods to change the fate of stem cells are adding growth factors or some signaling pathways. In recent years, many studies have revealed that the geometrical microenvironment played an essential role in regulating the fate of stem cells, and the physical factors of scaffolds including mechanical properties, pore sizes, porosity, surface stiffness, three-dimensional structures, and mechanical stimulation may affect the fate of stem cells. Chemical factors such as cell-adhesive ligands and exogenous growth factors would also regulate the fate of stem cells. Understanding how these physical and chemical cues affect the fate of stem cells is essential for building more complex and controlled scaffolds for directing stem cell fate.

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Section: Stiffness Effectsmentioning

confidence: 74%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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“…The Wnt signalling pathway is a highly conserved signalling pathway in cell evolution, playing an important role in several biological processes . Indeed, abnormal changes in Wnt signalling pathway are linked to a loss of bone mass, altered bone metabolism and osteoporosis .…”

Section: Discussionmentioning

confidence: 99%

Blockade of receptors of advanced glycation end products ameliorates diabetic osteogenesis of adipose‐derived stem cells through DNA methylation and Wnt signalling pathway

Zhang

Rao

et al. 2018

Cell Proliferation

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“…In the present study, DPSCs on the CS/β-GP hydrogel showed spherical shape. The related adhesion receptors need to be further investigated to identify the adhesion motility of DPSCs on the hydrogel [42,43].…”

Section: Stem Cells Internationalmentioning

confidence: 99%

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

Zhou

et al. 2019

Stem Cells International

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The pulpotomy with pulp capping is aimed at retaining vital pulp with reparative dentin formation. Vascular endothelial growth factor (VEGF) plays a crucial role in dentin regeneration; however, its constant administrations in the human body is still problematic. Chitosan was widely studied as an effective carrier to deliver bioactive molecules in regenerative medicine. In this study, we conducted a chitosan/β-glycerophosphate (CS/β-GP) hydrogel as a VEGF-sustained release system and explored its effects on dental pulp stem cells (DPSCs). CS/β-GP hydrogel was manufactured using a sol-gel method. SEM assay showed the spongy and porous microstructure of the lyophilized hydrogels. DPSCs cultured in the CS/β-GP hydrogel kept adhesion and vitality. CCK-8 assay tested the promoted proliferation activity of DPSCs on the hydrogel. Besides, the added VEGF protein could continually release from VEGF/CS/β-GP hydrogel. The VEGF/CS/β-GP hydrogel could promote the odontogenic differentiation of DPSCs better than VEGF treatment without hydrogel. Our results suggested that CS/β-GP hydrogel could continually release VEGF and contribute to odontogenic differentiation of DPSCs, thus may become a potential carrier of bioactive molecules in pulp capping therapy.

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Stiffness regulates the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells via the WNT signalling pathway

Abstract: Our results showed that mechanical factors can regulate the proliferation and differentiation of DPSCs via the WNT signalling pathway. This provides theoretical basis to optimize dental or bone tissue regeneration through increasing stiffness of extracelluar matrix.

Cited by 45 publications

References 43 publications

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Blockade of receptors of advanced glycation end products ameliorates diabetic osteogenesis of adipose‐derived stem cells through DNA methylation and Wnt signalling pathway

Evaluation of Chitosan Hydrogel for Sustained Delivery of VEGF for Odontogenic Differentiation of Dental Pulp Stem Cells

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