Stick or leave – Pushing methanogens to biofilm formation for ex situ biomethanation

Jensen, Marina Bergen; Strübing, Dietmar; Jonge, Nadieh de; Nielsen, Jeppe Lund; Ottosen, Lars Ditlev Mørck; Koch, Konrad; Kofoed, Michael Vedel Wegener

doi:10.1016/j.biortech.2019.121784

Cited by 37 publications

(8 citation statements)

References 40 publications

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“…In general, it is conceivable that strict acetotrophic methanogens (Methanothrix) coexist with homoacetogens in hydrogenotrophic communities. While a lower abundance of Firmicutes was reported in earlier studies [10,23], a similar abundance as in this study was found by other studies [25,52,53]. In contrast, Bacteroidetes was found to be the dominant phylum by another study [54].…”

Section: Microbial Community Structure and Dynamicssupporting

confidence: 90%

Microbial Resource Management for Ex Situ Biomethanation of Hydrogen at Alkaline pH

et al. 2020

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Biomethanation is a promising solution to convert H2 (produced from surplus electricity) and CO2 to CH4 by using hydrogenotrophic methanogens. In ex situ biomethanation with mixed cultures, homoacetogens and methanogens compete for H2/CO2. We enriched a hydrogenotrophic microbiota on CO2 and H2 as sole carbon and energy sources, respectively, to investigate these competing reactions. The microbial community structure and dynamics of bacteria and methanogenic archaea were evaluated through 16S rRNA and mcrA gene amplicon sequencing, respectively. Hydrogenotrophic methanogens and homoacetogens were enriched, as acetate was concomitantly produced alongside CH4. By controlling the media composition, especially changing the reducing agent, the formation of acetate was lowered and grid quality CH4 (≥97%) was obtained. Formate was identified as an intermediate that was produced and consumed during the bioprocess. Stirring intensities ≥ 1000 rpm were detrimental, probably due to shear force stress. The predominating methanogens belonged to the genera Methanobacterium and Methanoculleus. The bacterial community was dominated by Lutispora. The methanogenic community was stable, whereas the bacterial community was more dynamic. Our results suggest that hydrogenotrophic communities can be steered towards the selective production of CH4 from H2/CO2 by adapting the media composition, the reducing agent and the stirring intensity.

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Section: Microbial Community Structure and Dynamicssupporting

confidence: 90%

Microbial Resource Management for Ex Situ Biomethanation of Hydrogen at Alkaline pH

et al. 2020

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“…The CO 2 -BMP process can be employed in multiple applications such as biogas upgrading, power-to-gas applications, decentralized energy production, and for the conversion of H 2 /CO 2 of process flue gasses in waste to value concepts from, e.g., ethanol, petroleum, steel, and chemical industries 10 . There are two main approaches for CO 2 -BMP 11 : ex situ biomethanation using pure cultures 12,13 or enriched mixed cultures [14][15][16] , and in situ biomethanation 17,18 . In situ biomethanation is examined for upgrading the CH 4 content of biogas by adding H 2 to anaerobic digesters.…”

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confidence: 99%

Hyperthermophilic methanogenic archaea act as high-pressure CH4 cell factories

et al. 2021

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Bioprocesses converting carbon dioxide with molecular hydrogen to methane (CH4) are currently being developed to enable a transition to a renewable energy production system. In this study, we present a comprehensive physiological and biotechnological examination of 80 methanogenic archaea (methanogens) quantifying growth and CH4 production kinetics at hyperbaric pressures up to 50 bar with regard to media, macro-, and micro-nutrient supply, specific genomic features, and cell envelope architecture. Our analysis aimed to systematically prioritize high-pressure and high-performance methanogens. We found that the hyperthermophilic methanococci Methanotorris igneus and Methanocaldococcoccus jannaschii are high-pressure CH4 cell factories. Furthermore, our analysis revealed that high-performance methanogens are covered with an S-layer, and that they harbour the amino acid motif Tyrα444 Glyα445 Tyrα446 in the alpha subunit of the methyl-coenzyme M reductase. Thus, high-pressure biological CH4 production in pure culture could provide a purposeful route for the transition to a carbon-neutral bioenergy sector.

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“…Among the ex situ technologies reported, plug flow bioreactors [120] and biotrickling filters [121] are capable of reaching conversion efficiencies greater than 99% and biomethane productivities of up to 38-40 m 3 per m 3 of reactor and day (m 3 /mR 3 d). This configuration allows the effective adaptation of non-specific anaerobic cultures to the operating conditions of the upgrading [122] in which both hydrogenotrophic and acetotrophic archaea predominate as a consequence of the relevant role of homoacetogens in the formation of acetate as intermediate [123].…”

Section: Hydrogenotrophic Biogas Upgradingmentioning

confidence: 99%

Biogas from Anaerobic Digestion as an Energy Vector: Current Upgrading Development

Iglesias¹,

Muñoz

Fdz-Polanco

et al. 2021

Energies

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The present work reviews the role of biogas as advanced biofuel in the renewable energy system, summarizing the main raw materials used for biogas production and the most common technologies for biogas upgrading and delving into emerging biological methanation processes. In addition, it provides a description of current European legislative framework and the potential biomethane business models as well as the main biogas production issues to be addressed to fully deploy these upgrading technologies. Biomethane could be competitive due to negative or zero waste feedstock prices, and competitive to fossil fuels in the transport sector and power generation if upgrading technologies become cheaper and environmentally sustainable.

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Stick or leave – Pushing methanogens to biofilm formation for ex situ biomethanation

Cited by 37 publications

References 40 publications

Microbial Resource Management for Ex Situ Biomethanation of Hydrogen at Alkaline pH

Microbial Resource Management for Ex Situ Biomethanation of Hydrogen at Alkaline pH

Hyperthermophilic methanogenic archaea act as high-pressure CH4 cell factories

Biogas from Anaerobic Digestion as an Energy Vector: Current Upgrading Development

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