Steroids are required for epidermal cell fate establishment in
            <i>Arabidopsis</i>
            roots

Kuppusamy, Kavitha T.; Chen, Andrew Y.; Nemhauser, Jennifer L.

doi:10.1073/pnas.0811633106

Cited by 79 publications

(89 citation statements)

References 29 publications

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“…4). A model where BES1 acts to boost response to various stimuli is consistent with the role of brassinosteroids in auxin response analyzed here and the observed role of brassinosteroids in enhancing differential cell fate determination during root epidermal development (Kuppusamy et al, 2009).…”

Section: Discussionsupporting

confidence: 85%

Bipartite Promoter Element Required for Auxin Response

Walcher

Nemhauser

2011

Plant Physiology

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Multiple mechanisms have been described for coordination of responses to the plant hormones auxin and brassinosteroids (Zhang et al., 2009). One unexplained phenomenon is the reliance of the auxin transcriptional response on a functional brassinosteroid pathway. In this study, we used luciferase reporters to interrogate the promoter of SMALL AUXIN-UP RNA15 (SAUR15), a well-characterized auxin and brassinosteroid early response gene in Arabidopsis (Arabidopsis thaliana). After identifying a minimal region sufficient for auxin response, we targeted predicted cis-regulatory elements contained within this sequence and found a critical subset required for hormone response. Specifically, reporter sensitivity to auxin treatment required two elements: a Hormone Up at Dawn (HUD)-type E-box and an AuxRE-related TGTCT element. Reporter response to brassinosteroid treatment relied on the same two elements. Consistent with these findings, the transcription factors BRASSINOSTEROID INSENSITIVE1-EMS SUPPESSOR1 and MONOPTEROS (MP)/ AUXIN RESPONSE FACTOR5 (ARF5) showed enhanced binding to the critical promoter region containing these elements. Treatment with auxin or brassinosteroids could enhance binding of either transcription factor, and brassinosteroid enhancement of MP/ARF5 binding required an intact HUD element. Conservation of clustered HUD elements and AuxRE-related sequences in promoters of putative SAUR15 orthologs in a number of flowering plant species, in combination with evidence for statistically significant clustering of these elements across all Arabidopsis promoters, provided further evidence of the functional importance of coordinated transcription factor binding.

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Section: Discussionsupporting

confidence: 85%

Bipartite Promoter Element Required for Auxin Response

Walcher

Nemhauser

2011

Plant Physiology

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“…In addition, a number of experimental studies have shown that the same MYBs-BHLHs-TTG-GL2 network controls epidermal cell fate in both organs (Larkin et al, 2003;Schiefelbein et al, 2003;Serna 2004a;Serna, 2004b). In roots, brassinosteroids also promote root hair formation, acting upstream of the MYBs-BHLHs-TTG-GL2 network (Kuppusamy et al, 2009). Similar to what occurs in the hypocotyl epidermis, the loss of brassinosteroid signalling in the root results in random patterns of GL2 expression and in a strong reduction of CPC expression (Kuppusamy et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Relationship between brassinosteroids and genes controlling stomatal production in the Arabidopsis hypocotyl

Fuentes¹,

Cañamero²,

Serna³

2012

Int. J. Dev. Biol.

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Stomata are excellent model systems for examining the mechanisms that regulate cell fate determination and pattern formation. It has recently been demonstrated that brassinosteroids control stomatal development by regulating both the MAPK kinase kinase YODA and the basic helix-loop-helix transcriptional factor SPEECHLESS. Here, we show that these plant regulators positively regulate stomatal formation in the hypocotyl and also accelerate their development. Hormone tests, reporter gene studies and mutant analyses revealed that brassinosteroids act upstream of the transcriptional factors CAPRICE and GLABRA2. These plant regulators control an earlier stage of stomatal production than those regulated by the membrane receptor TOO MANY MOUTHS. This work highlights differences in the genetic control of stomatal development between cotyledons or leaves and hypocotyls.

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“…Beyond the overall plant dwarfism, detailed phenotypic analysis has permitted the characterization of tissue-specific defects in plant vasculature Ibañes et al, 2009;Fàbregas et al, 2010), pollen development (Ye et al, 2010), root hair patterning (Kuppusamy et al, 2009), and QC activity (González-García et al, 2011). Signal amplification from the BRI1 receptor implies the transcriptional regulation of thousands of genes that, as direct targets of BES1 and BRZ1, may act in different cell types regulating different responses (Sun et al, 2010;Yu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

Fàbregas

Boeren

et al. 2013

The Plant Cell

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Brassinosteroid (BR) hormones are primarily perceived at the cell surface by the leucine-rich repeat receptor-like kinase BRASSINOSTEROID INSENSITIVE1 (BRI1). In Arabidopsis thaliana, BRI1 has two close homologs, BRI1-LIKE1 (BRL1) and BRL3, respectively, which are expressed in the vascular tissues and regulate shoot vascular development. Here, we identify novel components of the BRL3 receptor complex in planta by immunoprecipitation and mass spectrometry analysis. Whereas BRI1 ASSOCIATED KINASE1 (BAK1) and several other known BRI1 interactors coimmunoprecipitated with BRL3, no evidence was found of a direct interaction between BRI1 and BRL3. In addition, we confirmed that BAK1 interacts with the BRL1 receptor by coimmunoprecipitation and fluorescence microscopy analysis. Importantly, genetic analysis of brl1 brl3 bak1-3 triple mutants revealed that BAK1, BRL1, and BRL3 signaling modulate root growth and development by contributing to the cellular activities of provascular and quiescent center cells. This provides functional relevance to the observed protein-protein interactions of the BRL3 signalosome. Overall, our study demonstrates that cell-specific BR receptor complexes can be assembled to perform different cellular activities during plant root growth, while highlighting that immunoprecipitation of leucine-rich repeat receptor kinases in plants is a powerful approach for unveiling signaling mechanisms with cellular resolution in plant development.

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Steroids are required for epidermal cell fate establishment in Arabidopsis roots

Abstract: brassinosteroids ͉ cell fate specification ͉ positional cues ͉ root epidermis

Cited by 79 publications

References 29 publications

Bipartite Promoter Element Required for Auxin Response

Bipartite Promoter Element Required for Auxin Response

Relationship between brassinosteroids and genes controlling stomatal production in the Arabidopsis hypocotyl

The BRASSINOSTEROID INSENSITIVE1–LIKE3 Signalosome Complex Regulates Arabidopsis Root Development

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