Steroid Hormone Receptors in the Uterus and Ovary of Immature Rats Treated with Gonadotropins.

Arakawa, Satoko; Iyo, Masato; Ohkawa, Ryo; Kambegawa, Akira; Okinaga, Shoichi; Arai, Kiyoshi

doi:10.1507/endocrj1954.36.219

Cited by 12 publications

(8 citation statements)

References 27 publications

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“…Specific estrogen binding exists in the ovaries of intact or hypophysectomized immature rats [17,18], and our results confirm the presence of E 2 receptors in the ovaries of intact immature hamsters and rats [9] and of rabbits, guinea pigs, and mice [10], but with variations in dissociation constant (Kd), number of binding sites, and percentage of occupancy. However, there have been only limited attempts to show estrogen binding sites in granulosa cells of intact antral follicles, i.e., they have been determined only for rat granulosa cells [19].…”

Section: Introductionsupporting

confidence: 81%

Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Kawashima

Greenwald

1993

Biology of Reproduction

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The hypophysectomized or intact hamster--unlike the rat--does not respond to estrogen by increasing the number of large antral follicles. In the pig, estrogen inhibits progesterone synthesis by granulosa cells from large follicles for 36 h before progesterone accumulation is enhanced. The objective of this study was to determine whether these divergent responses of the three species to estrogen are related to differences in follicular estrogen receptors. For the rat antral follicle, the equilibrium dissociation constants (Kds), expressed as nmol/L, were 0.55 +/- 0.07 in the cytosolic fraction and 0.46 +/- 0.11 in the nuclear fraction; for the hamster and pig follicles, the respective Kds were 1.08 +/- 0.04 and 0.94 +/- 0.11, and 1.16 +/- 0.23 and 1.09 +/- 0.17, respectively. The cytosolic and nuclear Kds for the rat follicle were statistically different from those of the other two species, but this is most likely not of biological consequence. Comparison of the maximal numbers of binding sites (NBSmax) in the cytosolic fractions of the antral follicles, expressed as fmol/mg protein, indicated a relationship of hamster > rat = pig; whereas in the nuclear fraction, with the NBSmax expressed as fmol/mg DNA, the relationship was hamster > pig > rat. The total number of estrogen receptors (sum of the NBSmax of cytosolic and nuclear fractions) was approximately tenfold higher in the hamster than in the rat. Thus, the failure of hamster large follicles to respond in vivo or in vitro to estrogen cannot be explained on the basis of lack of estrogen receptors.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Introductionsupporting

confidence: 81%

Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Kawashima

Greenwald

1993

Biology of Reproduction

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“…Studies localizing estrogen receptor protein and its mRNA to ovary are consistent with a local role for estradiol in modulating follicle growth and maturation through classical receptor-mediated pathways. In this respect, our knowledge is mostly derived from rodents (Richards, 1975;Kim and Greenwald 1987a,b;Arakawa et al, 1989;Hillier et al, 1989;Kawashima and Greenwald, 1993) and rabbits (Iwai et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Estrogen receptor protein and mRNA expression in the ovary of sheep

et al. 1997

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Using immunohistochemistry and in situ hybridization, we attempted to identify the estrogen receptor (ER) protein and messenger RNA (mRNA) in sheep ovaries during the follicular phase of the estrous cycle. Monoclonal anti‐ER antibodies H222 and 1D5 were used for localizing estrogen receptor on ovarian cryo‐sections. Labeling for ER was found over the nuclei of surface epithelium, interstitial tissue, and granulosa cells of small as well as large ovarian follicles. In the preantral and small antral follicles, intense nuclear ER labeling was observed in mural granulosa cells and particularly in cumulus/granulosa cells surrounding the oocyte. In the large healthy looking follicles, greater diversity in labeling for ER was observed, which is characterized by mixed populations of granulosa cells expressing positive and more or less negative nuclear labeling. Such a pattern of labeling was particularly evident in follicles showing the signs of atresia. Generally, more intense nuclear staining was localized in granulosa cells proximal to basal membrane. In situ hybridization studies revealed the presence of ER mRNA in ovarian tissue. Autoradiographic visualization localized ER mRNA expression over the granulosa cells of healthy follicles of all sizes. Level of hybridization signal was comparable in mural and cumulus granulosa cells. In atretic follicles, the level of hybridization signal in granulosa cells was comparable to that of healthy follicles. A relatively weaker level of labeling was observed in granulosa cells dispersed in follicular antrum in follicles with advanced atretic lesions. Theca cells expressed a lower level of labeling than granulosa cells. Specificity of labeling for both ER protein and mRNA in ovary was proved by parallel probing the ovine uterus. Ovine ER recognition by both H222 and 1D5 antibodies was also proved by immunoblotting. These studies demonstrate the presence of the estrogen receptor and its messenger RNA in the sheep ovary and suggest an autocrine/paracrine role of estradiol and its receptor in the regulation of ovarian follicle development in sheep. Mol. Reprod. Dev. 48:53–62, 1997. © 1997 Wiley‐Liss, Inc.

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“…2=). Pituitary gonadotropin has an important role in the maturation of secondary follicles in intact female rats (Arakawa et al, 1989). Thus, follicular development was retarded in mice treated with pituitary antigonadotropin for seven days at birth (Lunenfeld, 1969).…”

Section: Resultsmentioning

confidence: 99%

EFFECT OF NEONATAL OESTROGEN AND hCG TREATMENT ON THE GENITAL ORGANS OF ADULT FEMALE RATS

Nikolić

Mrvić

Ivanov

et al. 1999

Acta Veterinaria Hungarica

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The ovaries and the uterine as well as vaginal mucous membranes of 80-, 180-and 365-day-old intact female rats and females neonatally treated with a single dose of oestrogen and repeated doses of human chorionic gonadotropin (D+/ were studied. Numerous follicles, interstitial cells and corpora lutea (CL) were Key words: Rat, ovary, uterus, vagina, oestrogen, hCG The progressive decline of regular oestrous cycles and ovulatory functions with advancing age in female rats has been well documented in previous studies (Pantic and Lovren, 1981;Ennis and Davies, 1982;Nass et al., 1984). In female rats and mice, neonatal oestrogen treatment induces changes in the reproductive tract that may persist throughout the animals life (Pantic, 1981). These changes include a persistent rather than cyclic oestrus. During persistent oestrus, circulating oestradiol is present from many large ovarian follicles, but the luteinizing hormone surge mechanism is inhibited and ovulation does not occur (Leung, 1978;Pantic and Lovren, 1981).Studies on abnormalities of the reproductive tract indicate that oestrogens have inhibitory effects on ovarian steroidogenesis (Groen-Clevant, 1981;Wada et al., 1984). The uterus and vagina are also dependent on the effect of hormones administered to neonates (Ennis and Davies, 1982;Keys and King, 1989).In this paper, attention is focused on the ovarian follicles and interstitial tissue and on the properties of uterus and vagina in adult rats neonatally treated with oestrogen and human chorionic gonadotropin (hCG).

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Steroid Hormone Receptors in the Uterus and Ovary of Immature Rats Treated with Gonadotropins.

Cited by 12 publications

References 27 publications

Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Comparison of Follicular Estrogen Receptors in Rat, Hamster, and Pig1

Estrogen receptor protein and mRNA expression in the ovary of sheep

EFFECT OF NEONATAL OESTROGEN AND hCG TREATMENT ON THE GENITAL ORGANS OF ADULT FEMALE RATS

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