Steric Interference of Adhesion Supports In-Vitro Chondrogenesis of Mesenchymal Stem Cells on Hydrogels for Cartilage Repair

Goldshmid, Revital; Cohen, Shlomit; Shachaf, Yonatan; Kupershmit, Ilana; Sarig-Nadir, Offra; Seliktar, Dror; Wechsler, R J

doi:10.1038/srep12607

Cited by 21 publications

(14 citation statements)

References 45 publications

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“…The results indicated that cartilage ECM was deposited in the hydrogel, adjacent cartilage tissue growth was facilitated by MSCs, and significantly more cartilage tissue formation was found compared to the control group with microfracture treatment. Another composite hydrogel based on PEGDA-fibrinogen (commercially known as GelrinC™ for cartilage repair) has been shown to support enhanced chondrogenesis of MSCs with minimizing hypertrophy [184], which suggested that composite hydrogels can be designed for improved ability to regenerate cartilage tissues.…”

Section: Designing Hydrogels For Reconstruction Of Osteochondral Imentioning

confidence: 99%

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Cell-laden hydrogels for osteochondral and cartilage tissue engineering

et al. 2017

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Despite tremendous advances in the field of regenerative medicine, it still remains challenging to repair the osteochondral interface and full-thickness articular cartilage defects. This inefficiency largely originates from the lack of appropriate tissue engineered artificial matrices that can replace the damaged regions and promote tissue regeneration. Hydrogels are emerging as a promising class of biomaterials for both soft and hard tissue regeneration. Many critical properties of hydrogels, such as mechanical stiffness, elasticity, water content, bioactivity, and degradation, can be rationally designed and conveniently tuned by proper selection of the material and chemistry. Particularly, advances in the development of cell-laden hydrogels have opened up new possibilities for cell therapy. In this article, we describe the problems encountered in this field and review recent progress in designing cell-hydrogel hybrid constructs for promoting the reestablishment of osteochondral/cartilage tissues. Our focus centers on the effects of hydrogel type, cell type, and growth factor delivery on achieving efficient chondrogenesis and osteogenesis. We give our perspective on developing next-generation matrices with improved physical and biological properties for osteochondral/cartilage tissue engineering. We also highlight recent advances in biomanufacturing technologies (e.g. molding, bioprinting, and assembly) for fabrication of hydrogel-based osteochondral and cartilage constructs with complex compositions and microarchitectures to mimic their native counterparts.

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Section: Designing Hydrogels For Reconstruction Of Osteochondral Imentioning

confidence: 99%

“…Commercially available hydrogel biomaterials for CTE [312] include collagen, fibroin, and HA. PEG [183, 313] and PEG-fibroin [184, 314] hydrogels have progressed to clinical testing. There are still some barriers to the clinical translation of the more complex hydrogel based constructs.…”

Section: Challenges Of Cell-laden Hydrogel Strategies For Oce and Otementioning

confidence: 99%

Cell-laden hydrogels for osteochondral and cartilage tissue engineering

et al. 2017

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“…UV irradiation is also normally used on engineered cartilage. A number of studies have shown the substrate’s physical properties and its topological structures to be critical for the chondrocyte’s differentiation, redifferentiation, and maturation [246]. A suitable substrate for seeding cells may help cartilage regeneration through mechanical, biological, and chemical effects in cartilage regeneration [247].…”

Section: Physical Stimulation For Cartilage Regenerationmentioning

confidence: 99%

Physical Stimulations for Bone and Cartilage Regeneration

Huang

Das

Patel

et al. 2018

Regen. Eng. Transl. Med.

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A wide range of techniques and methods are actively invented by clinicians and scientists who are dedicated to the field of musculoskeletal tissue regeneration. Biological, chemical, and physiological factors, which play key roles in musculoskeletal tissue development, have been extensively explored. However, physical stimulation is increasingly showing extreme importance in the processes of osteogenic and chondrogenic differentiation, proliferation and maturation through defined dose parameters including mode, frequency, magnitude, and duration of stimuli. Studies have shown manipulation of physical microenvironment is an indispensable strategy for the repair and regeneration of bone and cartilage, and biophysical cues could profoundly promote their regeneration. In this article, we review recent literature on utilization of physical stimulation, such as mechanical forces (cyclic strain, fluid shear stress, etc.), electrical and magnetic fields, ultrasound, shock waves, substrate stimuli, etc., to promote the repair and regeneration of bone and cartilage tissue. Emphasis is placed on the mechanism of cellular response and the potential clinical usage of these stimulations for bone and cartilage regeneration.

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“…In a three‐dimensional poly(ethylene glycol) (PEG) hydrogel, the carboxylic group chemically tethered on PEG network was found to support the best chondrogenic differentiation of human MSCs among a series of small chemical groups . Most recently, the design of surface chemistry to prevent cell adhesion and induce cell aggregation has been reported to promote chondrogenesis of MSCs . For instance, by employing two polyelectrolytes with opposite charges, poly( l ‐glutamic acid) and chitosan, a hydrophilic surface in three‐dimensional scaffolds was developed and aggregation of rabbit adipose‐derived MSCs was encouraged, showing improved chondrogenic differentiation .…”

Section: Introductionmentioning

confidence: 99%