Stem Cells and Tissue Engineering-Based Therapeutic Interventions: Promising Strategies to Improve Peripheral Nerve Regeneration

Assis, Ana Carolina Correa de; Reis, Amanda Luiza Silva; Nunes, Leonardo Vieira; Ferreira, Luiz Fernando Romanholo; Bilal, Muhammad; Iqbal, Hafiz M.N.; Soriano, Renato Nery

doi:10.1007/s10571-022-01199-3

Cited by 7 publications

(8 citation statements)

References 85 publications

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“…The SCF + DPSCs group was significantly higher than the DPSCs and CST groups and reduced compared to the Autograft group in the expression of CD31, indicating SCF combined with DPSCs promoted facial nerve-vascularized regeneration, which was consistent with the result in vitro and verified that pluripotent cells could increase functional recovery and revascularization in Assis’s study. 64 At 12 weeks postoperatively, the chitosan tube protecting the regenerated facial nerve was surrounded by connective tissue containing vascular structures. This connective tissue was not easy to peel off, which affected the positive expression CD31 to some extent.…”

Section: Discussionmentioning

confidence: 99%

“…To explore the vascularization of regenerated facial nerve harvested from the SCF + DPSCs group, the expression of CD31 associated with the vascular endothelial cell was assessed by western blot at 12 weeks after the operation. The SCF + DPSCs group was significantly higher than the DPSCs and CST groups and reduced compared to the Autograft group in the expression of CD31, indicating SCF combined with DPSCs promoted facial nerve-vascularized regeneration, which was consistent with the result in vitro and verified that pluripotent cells could increase functional recovery and revascularization in Assis’s study . At 12 weeks postoperatively, the chitosan tube protecting the regenerated facial nerve was surrounded by connective tissue containing vascular structures.…”

Section: Discussionmentioning

confidence: 99%

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Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in Rabbits

Liu

et al. 2022

ACS Omega

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Facial nerve injury is a common clinical condition that leads to disfigurement and emotional distress in the affected individuals, and the recovery presents clinical challenges. Tissue engineering is the standard method to repair nerve defects. However, nerve regeneration is still not satisfactory because of poor neovascularization after implantation, especially for the long-segment nerve defects. In the current study, we aimed to investigate the potential of chitosan tubes inoculated with stem cell factor (SCF) and dental pulp stem cells (DPSCs) in facial nerve-vascularized regeneration. In the in vitro experiment, DPSCs were isolated, cultured, and then identified. The optimal concentration of SCF was screened by CCK8. Cytoskeleton and living-cell staining, migration, CCK8 test, and neural differentiation assays were performed, revealing that SCF promoted the biological activity of DPSCs. Surprisingly, SCF increased the neural differentiation of DPSCs. The migration and angiogenesis experiments were carried out to show that SCF promoted the angiogenesis and migration of human umbilical vein endothelial cells (HUVECs). In the facial nerve, 7 mm defects of New Zealand white rabbits, hematoxylin–eosin (HE), immunohistochemistry, toluidine blue staining, and transmission electron microscopy observation were performed at 12 weeks postsurgery to show more nerve fibers and better myelin sheath in the SCF + DPSC group. In addition, the whisker movements, Masson’s staining, and western blot assays were performed, demonstrating functional repair and that the expression level of CD31 protein in the group SCF + DPSCs was relatively close to that in the group Autograft. In summary, chitosan tubes inoculated with SCF and DPSCs increased neurovascularization and provided an effective method for repairing facial nerve defects, indicating great promise for clinical application.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in Rabbits

Liu

et al. 2022

ACS Omega

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“…Research indicates that NGCs combined with cells (e.g., SCs, macrophages, and stem cells) and neurotrophic factors are highly effective in promoting axonal growth. This combination (NGCs, cells and stimuli) serves both as a scaffold and a carrier of molecular and mechanical signals in the process of regeneration, thus offering a prospective method of peripheral nerve repair ( de Assis et al, 2022 ). SCs are the most used cells in NGCs to make the biomaterial regrow axons faster and more effectively ( Berrocal et al, 2013 ).…”

Section: The Potential Of Exogenous Transforming Growth Factor-β In T...mentioning

confidence: 99%

“…Thus, TGF-β can be used in NGCs to promote the polarization of macrophages into the M2 phenotype. Difficulties in obtaining and cultivating SCs limit their direct use, and therefore, stem cells can act as a source of SC-like cells in NGCs ( de Assis et al, 2022 ). Notably, TGF-β is also found to improve the survival and pro-regenerative capacity of stem cells.…”

Section: The Potential Of Exogenous Transforming Growth Factor-β In T...mentioning

confidence: 99%

Role of Transforming Growth Factor Beta in Peripheral Nerve Regeneration: Cellular and Molecular Mechanisms

Wei

Zhan

et al. 2022

Front. Neurosci.

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Peripheral nerve injury (PNI) is one of the most common concerns in trauma patients. Despite significant advances in repair surgeries, the outcome can still be unsatisfactory, resulting in morbidities such as loss of sensory or motor function and reduced quality of life. This highlights the need for more supportive strategies for nerve regrowth and adequate recovery. Multifunctional cytokine transforming growth factor-β (TGF-β) is essential for the development of the nervous system and is known for its neuroprotective functions. Accumulating evidence indicates its involvement in multiple cellular and molecular responses that are critical to peripheral nerve repair. Following PNI, TGF-β is released at the site of injury where it can initiate a series of phenotypic changes in Schwann cells (SCs), modulate immune cells, activate neuronal intrinsic growth capacity, and regulate blood nerve barrier (BNB) permeability, thus enhancing the regeneration of the nerves. Notably, TGF-β has already been applied experimentally in the treatment of PNI. These treatments with encouraging outcomes further demonstrate its regeneration-promoting capacity. Herein, we review the possible roles of TGF-β in peripheral nerve regeneration and discuss the underlying mechanisms, thus providing new cues for better treatment of PNI.

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“…Studies indicate the increasing number of PN problems in the form of sensory/motor functions diseases caused by traumatic injuries, spinal cord injuries, birth defects, etc., which are approximately considered irreversible. , Despite the possibility of better treatment of PN injuries compared to central nerves, , if the injuries are more than 10–15 mm long, it is very difficult to rehabilitate their function through axon innervation. In this regard, tissue engineering is attempted to reduce sensory/motor function problems quickly with high efficiency, with the physiological approaches of nerve healing through electrical or optical stimuli, decellularized nerve cell transplantation, and ultimately generating nerves from stem cells . Autologous PN transplantation has been recommended as a gold standard in clinical activities due to its high biocompatibility, proper orientation of the axon structure, and suitable ECM with very little transplant rejection .…”

Section: Introductionmentioning

confidence: 99%

Exploring the Physicochemical, Electroactive, and Biodelivery Properties of Metal Nanoparticles on Peripheral Nerve Regeneration

Sharifi

Kamalabadi-Farahani

Salehi

et al. 2022

ACS Biomater. Sci. Eng.

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Despite the advances in the regeneration/rehabilitation field of damaged tissues, the functional recovery of peripheral nerves (PNs), especially in a long gap injury, is considered a great medical challenge. Recent progress in nanomedicine has provided great hope for PN regeneration through the strategy of controlling cell behavior by metal nanoparticles individually or loaded on scaffolds/conduits. Despite the confirmed toxicity of metal nanoparticles due to long-term accumulation in nontarget tissues, they play a role in the damaged PN regeneration based on the topography modification of scaffolds/conduits, enhancing neurotrophic factor secretion, the ion flow improvement, and the regulation of electrical signals.Determining the fate of neural progenitor cells would be a major achievement in PN regeneration, which seems to be achievable by metal nanoparticles through altering cell vital approaches and controlling their functions. Therefore, in this literature, an attempt was made to provide an overview of the effective activities of metal nanoparticles on the PN regeneration, until the vital clues of the PN regeneration and how they are changed by metal nanoparticles are revealed to the researcher.

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Stem Cells and Tissue Engineering-Based Therapeutic Interventions: Promising Strategies to Improve Peripheral Nerve Regeneration

Cited by 7 publications

References 85 publications

Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in Rabbits

Chitosan Tubes Inoculated with Dental Pulp Stem Cells and Stem Cell Factor Enhance Facial Nerve-Vascularized Regeneration in Rabbits

Role of Transforming Growth Factor Beta in Peripheral Nerve Regeneration: Cellular and Molecular Mechanisms

Exploring the Physicochemical, Electroactive, and Biodelivery Properties of Metal Nanoparticles on Peripheral Nerve Regeneration

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