Stem Cells and Endometrial Regeneration: From Basic Research to Clinical Trial

Zhu, Xinxin; Péault, Bruno; Yan, Guijun; Sun, Haixiang; Hu, Yali; Ding, Lijun

doi:10.2174/1574888x14666181205120110

Cited by 45 publications

(38 citation statements)

References 99 publications

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“…When compared with traditional MSCs, ERCs have certain advantages, such as abundant resource, noninvasive collection methods, reusing human waste, and easy to large-scale expansion capability. In addition, ERCs were also reported with the ability in modulating immune response and lack of immunogenicity [11]. Previous studies from our research group and others have reported that ERC-mediated stem cell therapy could modulate the immune response in the treatment of a series of diseases, such as stroke, myocardial infarction, ischemia reperfusion injury, inflammatory bowel disease, and multiple organ dysfunction syndrome [12].…”

Section: Introductionmentioning

confidence: 95%

Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice

Zhao

et al. 2020

Stem Cell Res Ther

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Background Endometrial regenerative cells (ERCs), a novel type of mesenchymal-like stem cells, were identified as an attractive candidate for immunoregulation and induction of cardiac allograft tolerance. However, the underlying mechanisms of ERCs in immune regulation still remain largely unclear. The present study is designed to determine whether the expression of Galectin-9 (Gal-9), a soluble tandem-repeat member of the galectin family, is crucial for ERC-based immunomodulation. Methods In this study, we measured Gal-9 expression on ERCs and then co-cultured Gal-9-ERCs, ERCs, and ERCs+lactose (Gal-9 blocker) with activated C57BL/6-derived splenocytes. Furthermore, we performed mouse heart transplantation between BALB/c (H-2d) donor and C57BL/6 (H-2b) recipient. ERCs were administrated 24 h after the surgery, either alone or in combination with rapamycin. Results Our data demonstrate that ERCs express Gal-9, and this expression is increased by IFN-γ stimulation in a dose-dependent manner. Moreover, both in vitro and in vivo results show that Gal-9-ERC-mediated therapy significantly suppressed Th1 and Th17 cell response, inhibited CD8+ T cell proliferation, abrogated B cell activation, decreased donor-specific antibody production, and enhanced the Treg population. The therapeutic effect of ERCs was further verified by their roles in prolonging cardiac allograft survival and alleviating graft pathological changes. Conclusions Taken together, these data indicate that Gal-9 is required for ERC-mediated immunomodulation and prevention of allograft rejection.

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Section: Introductionmentioning

confidence: 95%

Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice

Zhao

et al. 2020

Stem Cell Res Ther

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“…After the damaged hEndoSC coculturing with MSCs in vitro, MSCs significantly increased the rate of proliferation and decreased apoptosis [9]. And many patients had already been pregnant after the transplantation of MSCs with collagen scaffolds [10,11].…”

Section: Introductionmentioning

confidence: 99%

Exosomes Derived from Umbilical Cord Mesenchymal Stem Cells Alleviate Mifepristone-Induced Human Endometrial Stromal Cell Injury

Wang

Xing

et al. 2020

Stem Cells International

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The human endometrial stromal cells (hEndoSCs) could maintain endometrial homeostasis and play a critical role in repairing endometrial injury. Mesenchymal stem cells (MSCs) significantly increase the proliferation of damaged hEndoSCs and protect them from apoptosis. Recent studies indicated that exosomes derived from stem cells could be recruited to damaged tissues for regeneration, which exhibit the potential for stem cell therapy as therapeutic vectors. In this study, we isolated human umbilical cord mesenchymal stem cell-derived exosomes (hUCMSC-Exos) and investigated the effects of hUCMSC-Exos on mifepristone-induced hEndoSC injury. Exosome uptake and cell proliferation as well as cell apoptosis of damaged hEndoSCs treated with hUCMSC-Exos were detected. We also assessed the expression of apoptosis-related proteins and the PTEN/AKT signaling pathway. We found hUCMSC-Exos improved the proliferation of damaged hEndoSCs and protected hEndoSCs from the mifepristone-induced apoptosis. hUCMSC-Exos upregulated Bcl-2 level as well as downregulated Cleaved Caspase-3 level and activated the PTEN/AKT signaling pathway to regulate the proliferation and antiapoptosis. These results indicated hUCMSC-Exos protected hEndoSCs from mifepristone-induced apoptosis and played an active role in repairing the damaged hEndoSCs through the PTEN/AKT signaling pathway in vitro. hUCMSC-Exos may hold great promise in the cell-free therapy of endometrial injury.

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“…As stated by Padykula, 28 the resident stem-like cell population migrates and gives rise to a group of progenitor cells that become committed to specific types of cell differentiation, for example, epithelial, stromal, and vascular cells, within certain microenvironments. 29 Studies of the various endometrial progenitor/stem cells and their markers have been reviewed by others 5,[30][31][32] and are outlined in Table 1. Endometrial stem cells have been described as a powerful tool for clinical and therapeutic applications.…”

Section: Stem Cell Populations In the Human Endometriummentioning

confidence: 99%

Microphysiological Modeling of the Human Endometrium

Campo

Murphy

Yıldız

et al. 2020

Tissue Engineering Part A

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Since the beginning of clinical medicine, the human uterus has held the fascination of clinicians and researchers, given its critical role in the reproduction of our species. The endometrial lining provides residence for the embryo; however, this symbiotic interaction can be disrupted if the timing is not correct and the endometrium is not receptive. Diseases associated with the endometrium interfere with the reproductive process and cause a life-altering burden of pain and even death. With the advancement of technologies and new insights into the biology of the endometrium, much has been uncovered about the dynamic and essential changes that need to occur for normal endometrial function, as well as aberrations that lead to endometrial diseases. As expected, the more that is uncovered, the more the complexity of the endometrium is made evident. In this study, we bring together three areas of scientific advancement that remain in their infancy, but which together have the potential to mirror this complexity and enable understanding. Studies on induced pluripotent stem cells, threedimensional tissue mimics, and microfluidic culture platforms will be reviewed with a focus on the endometrium. These unconventional approaches will provide new perspectives and appreciation for the elegance and complexity of the endometrium.

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Stem Cells and Endometrial Regeneration: From Basic Research to Clinical Trial

Cited by 45 publications

References 99 publications

Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice

Galectin-9 is required for endometrial regenerative cells to induce long-term cardiac allograft survival in mice

Exosomes Derived from Umbilical Cord Mesenchymal Stem Cells Alleviate Mifepristone-Induced Human Endometrial Stromal Cell Injury

Microphysiological Modeling of the Human Endometrium

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