Stem Cell Tracing Through MR Molecular Imaging

Yahyapour, Rasoul; Farhood, Bagher; Graily, Ghazale; Rezaeyan, Abolhasan; Rezapoor, Saeed; Abdollahi, Hamid; Cheki, Mohsen; Amini, Peyman; Fallah, Hengameh; Najafi, Masoud; Motevaseli, Elahe

doi:10.1007/s13770-017-0112-8

Cited by 30 publications

(20 citation statements)

References 101 publications

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“…Another key issue after MSC transplantation is how to track cell migration and survival in vivo. Molecular imaging can dynamically and quantitatively track biological processes, which provides the possibility for realtime monitoring of treatment response and non-invasive evaluation of treatment mechanisms [15,16]. Compared with other imaging methods, the bioluminescence imaging (BLI) system can real-time and quantitatively monitor the migration, differentiation, and proliferation of transplanted cells.…”

Section: Introductionmentioning

confidence: 99%

IGF-1C domain–modified hydrogel enhanced the efficacy of stem cells in the treatment of AMI

et al. 2020

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Background: Due to the low survival rate of cell transplantation, stem cell has not been widely used in clinical treatment of acute myocardial infarction (AMI). In this study, we immobilized the C domain peptide of insulin-like growth factor-1 on chitosan (CS-IGF-1C) to obtain bioactive hydrogel. The purpose was to investigate whether CS-IGF-1C hydrogel incorporated with human placenta-derived mesenchymal stem cells (hP-MSCs) can boost the survival of hP-MSCs and enhance their therapeutic effects. Methods: hP-MSCs, which continuously expressed green fluorescent protein (GFP) and firefly luciferase (Fluc), were transplanted with CS-IGF-1C hydrogel into a mouse myocardial infarction model. Cell survival was detected by bioluminescence imaging (BLI), and cardiac function was measured by echocardiogram. Real-time PCR and histological analysis were used to explore the therapeutic mechanism of CS-IGF-1C hydrogel. Results: CS-IGF-1C hydrogel could induce the proliferation of hP-MSCs and exert anti-apoptotic effects in vitro. The Calcine-AM/PI staining results showed that hP-MSCs seeded on CS-IGF-1C hydrogel could protect neonatal mouse ventricular cardiomyocytes (NMVCs) against oxidative stress. It was observed by BLI that CS-IGF-1C hydrogel injected into ischemic myocardium could improve the survival rate of hP-MSCs. Histology analysis indicated that cotransplantation of the CS-IGF-1C hydrogel and hP-MSCs could increase angiogenesis, reduce collagen deposition, ameliorate left ventricular expanded, and further promote the recovery of cardiac function. Besides, we found that the inflammatory response was inhibited and the expression of apoptosis-related genes was downregulated by CS-IGF-1C hydrogel. Conclusions: CS-IGF-1C hydrogel provides a conducive microenvironment for cells and significantly boosts the survival of hP-MSCs in mouse myocardial infarction model, which suggest that it may be a potential candidate for prolonging the therapeutic effect of hP-MSCs during AMI.

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Section: Introductionmentioning

confidence: 99%

IGF-1C domain–modified hydrogel enhanced the efficacy of stem cells in the treatment of AMI

et al. 2020

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“…MRI has a high spatial and temporal resolution, no tissue penetrating limit, and no radiation, but the disadvantages include relatively low sensitivity and low contrast, requiring a high load of cells with a magnetic label and comparatively long imaging times, with the possibility of affecting cell viability or biological interaction with the contrast agent [43,[62][63][64]. In this review, three studies used HSC tracking analysis by MRI with different magnetic fields, in which the highest magnetic field (17.6 Teslas) provided longer follow-ups (14 days after transplantation) when using low nanoparticle concentrations and incubation times in the cell labeling process.…”

Section: Discussionmentioning

confidence: 99%

“…Regarding the molecular imaging techniques approaches in this review, their physical principles, applicability, advantages, and limitations ( Figure 3) [43,46,47,[68][69][70][71][72][73], show a wide potentiality not only for in vitro studies and pre-clinical applications but also in the translation of some techniques in clinical studies, such as nuclear images (PET and SPECT) and MRI [47,73]. Comparing the molecular imaging technique characteristics and the HSC tracking evaluation shown in Figure 3, the optical techniques allowed a live cell tracking for a long period of time by BLI (at 1 day to 12 months)) [9,11,[16][17][18][19][20] and short time by FLI (at 30 min to 6 days) [21,[23][24][25], but in the latter, the signal is not necessarily from living cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, the HSCT tracking time depends on the radioisotope half-life, resulting in a small temporal window in signal detection. These techniques have good sensitivity, high penetration without depth limit, and can be translated into clinical practice, but they have a low spatial resolution, require the use of ionizing radiation, and have a high cost [14,42,43].…”

Section: Introductionmentioning

confidence: 99%

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Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Oliveira

Nucci

Filgueiras

et al. 2020

Cells

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The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis.

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“…Non-invasive in vivo imaging techniques including optical imaging, positron emission tomography (PET), magnetic resonance imaging (MRI), and computed tomography (CT) have been used to monitor tissue growth for obtaining longitudinal and quantitative tissue regeneration information in the same animal. Therefore, a strategy for the right combination of molecular imaging techniques and targeted contrast agents has been further applied for diagnosis and therapy of various diseases [1][2][3].…”

mentioning

confidence: 99%