Stem Cell Tissue Engineering and Regenerative Medicine

Chen, Bin; Liebman, Caleb; Rabbani, Parisa; Cho, Michael

doi:10.1002/9781119193272.ch1

Cited by 4 publications

(2 citation statements)

References 65 publications

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“…Regenerative nanomedicine has been widely accepted which has an objective to develop the compatible biomaterials to interact with cells/tissues present in the site of implantation [1][2][3]. Tissue engineering and regenerative medicine (TERM) exploit properties taken from the life sciences, engineering and physical sciences [4][5][6][7][8][9][10], and is therefore not only a highly interdisciplinary field of research but in practice composed of many novel and speciality areas [11]. Humans in their lifetime experience a number of acute diseases and traumas that affect cells, tissues and organs [4,6], which leads to the degeneration of living cells and tissues, or the malfunctioning of an entire organ system [7].…”

Section: Introductionmentioning

confidence: 99%

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Iqbal

Khan

Asif

et al. 2018

International Materials Reviews

145

114

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Tissue engineering and regenerative medicine are emerging as future approaches for the treatment of acute and chronic diseases. However, many challenging clinical conditions exist today and include congenital disorders, trauma, infection, inflammation and cancer, in which hard and soft tissue damage, organ failure and loss are still not treated effectively. Regenerative medicine has contributed to a number of innovations through artificial implants and biomedical materials, with advances are continually being made. Researchers are constantly developing new biomaterials and tissue engineered technologies to stimulate tissue regeneration in order to repair and replace damaged or malfunctioning organs. However, the challenge continues to lie in devising effective biomedical materials that can be implanted as scaffolds. Various approaches are emerging, according to the organ, tissue, disease and disorder. Scaffolds are implanted cell-free, or incorporated with stems cells, committed cells, or bioactive molecules. Irrespective, engineered biomaterials are required to regenerate and ultimately reproduce the original physiological, biological, chemical and mechanical properties over time. This is enabled by providing a three-dimensional architecture for cells to adhere, migrate, proliferate within, and differentiate appropriately for the growth of new tissues to provide a relevant structure, and in so doing, restore function. Biodegradable materials have been used extensively as regenerative therapies since their advent in early 20th century. One notable example is the development of surgical fixation devices. The selection, design and physicochemical properties of these materials are important and must consider biocompatibility, biodegradability and minimal cytotoxicity in the host to enable cell-proliferation, cell-matrix interactions and intercellular signalling for stimulating tissue growth. In this review, we critique the most studied and recently developed biodegradable

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Section: Introductionmentioning

confidence: 99%

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Iqbal

Khan

Asif

et al. 2018

International Materials Reviews

145

114

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show abstract

“…T issue engineering and regenerative medicine (TERM) has the immense therapeutic potential to treat a wide range of medical conditions from orthopedic injuries to the liver and cardiovascular diseases, traumatic brain injuries, diabetes, etc. [1][2][3][4][5] TERM combines biocompatible scaffolds with appropriate cells and devises cell growth conditions to create an engineered graft that can replace or repair damaged tissue. 6 Oxygen is an important vital molecule to sustain aerobic life forms.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Absolute Electron Paramagnetic Resonance Oxygen Imaging for the Assessment of Tissue Graft Oxygenation

Kotecha

Epel

Ravindran

et al. 2018

Tissue Engineering Part C: Methods

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Oxygen is the single most important molecule for sustaining life and, therefore, an important variable in tissue engineering and regenerative medicine. It has been shown that the change in oxygen concentration in an artificial or tissue-engineered graft affects cell survival, differentiation, and tissue growth in profound ways. However, at present, there are no reliable methods to map partial oxygen pressure (pO) in growing artificial tissues. Here, we adapt and test the suitability of electron paramagnetic resonance oxygen imaging (EPROI) in assessing tissue graft oxygenation in vitro. EPROI is an established method to assess absolute pO and has been widely applied to study tumor hypoxia in small animals. In this study, we demonstrate the feasibility of EPROI in evaluating oxygen dynamics in tissue grafts. We measured oxygen concentration in mesenchymal stem cell (MSC)-seeded polylactic-co-glycolic acid (PLGA) scaffolds with variable porosity. The pO maps of these scaffolds showed that the mean pO inside the scaffolds was smaller than the ambient air pO (21% oxygen, 160 torr) and was gradually increased with increasing pore size. We assessed the local oxygen dynamics of the MSC-seeded osteogenic scaffold made from collagen-chitosan hydrogels in a partially sealed Eppendorf tube. The change in pO values as a function of time inside the graft showed that the cells had used available oxygen within first 2 h of the experiment and then went to a dormant low oxygen consumption state until the oxygen supply was reestablished. Collectively, these data suggest that EPROI could be successfully used for mapping pO in tissue-engineered grafts. The knowledge of tissue graft oxygenation may be used to improve scaffold design and to assess the tissue viability and growth.

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In vivo preclinical cancer and tissue engineering applications of absolute oxygen imaging using pulse EPR

Epel

Kotecha

Halpern

2017

Journal of Magnetic Resonance

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Stem Cell Tissue Engineering and Regenerative Medicine

Cited by 4 publications

References 65 publications

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Recent concepts in biodegradable polymers for tissue engineering paradigms: a critical review

Noninvasive Absolute Electron Paramagnetic Resonance Oxygen Imaging for the Assessment of Tissue Graft Oxygenation

In vivo preclinical cancer and tissue engineering applications of absolute oxygen imaging using pulse EPR

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