Stem Cell Therapy: Current Applications and Potential for Urology

Wiafe, Bridget; Metcalfe, Peter; Adesida, Adetola B

doi:10.1007/s11934-015-0551-5

Cited by 9 publications

(5 citation statements)

References 93 publications

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“…MSCs are stromal cells that can be harvested from bone marrow, adipose tissue, and umbilical cords [26–28]. These cells have been regarded as prospective therapeutic tools because of their characteristics, such as multipotent differentiation, self‐renewal, immunomodulation, and immunoprivilege [29]. Among these characteristics, the immunomodulatory capacity of MSCs is currently the most important issue because this function of MSCs has been successfully applied in many inflammatory and autoimmune diseases.…”

Section: Discussionmentioning

confidence: 99%

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

Gao

Cen

Wang

et al. 2016

Stem Cells Translational Medicine

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Mesenchymal stem cells (MSCs) have been extensively investigated as a promising approach to treat many autoimmune and inflammatory diseases. The stress condition would affect the therapeutic efficacy and induce autophagy of MSCs. However, whether autophagy would affect the immunosuppressive capacity of MSCs is largely unknown. The present study aimed to assess whether autophagy plays an important role in regulating the immunomodulation of MSCs and the undermechanisms. We successfully inhibited and induced autophagy of MSCs using 3-methyladenine (3-MA) and rapamycin, respectively. Our results demonstrated that rapamycin strengthened the capacity of MSCs to inhibit CD4 + T-cell proliferation, whereas 3-MA weakened the inhibitory ability of MSCs. Mechanistically, 3-MA-pretreated MSCs secreted less, whereas rapamycin-pretreated MSCs secreted more transforming growth factor-b1 (TGF-b1) compared with the control cells. Furthermore, exogenous TGF-b1 addition recovered the immunosuppressive capacity of 3-MA-pretreated MSCs, whereas exogenous anti-TGF-b1 antibody addition reduced the immunosuppressive capacity of rapamycin-pretreated MSCs. These results indicated that the autophagy level regulates the immunosuppression of CD4 + T cells by MSCs through affecting TGF-b1 secretion and provides a novel method for improving the therapeutic efficacy of MSCs by activating autophagy. STEM CELLS TRANSLATIONAL MEDICINE 2016;5:1496-1505 SIGNIFICANCEMesenchymal stem cell (MSC)-based therapy is a promising tool to treat many diseases. Autophagy occurred in MSCs during their application, especially in those exposed to stress conditions. However, whether autophagy will affect the therapeutic efficacy of MSCs is largely unknown. This study makes a significant contribution to demonstrate that autophagy could improve the immunosuppression of CD4 + T cells by mesenchymal stem cells through transforming growth factor-b1. Therefore, regulation of autophagy in MSCs would provide a promising strategy to improve the therapeutic efficacy of these cells.

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Section: Discussionmentioning

confidence: 99%

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

Gao

Cen

Wang

et al. 2016

Stem Cells Translational Medicine

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“…Nevertheless, innovative and minimal-invasive alternatives have been developed, in particular, during the last decade, which compete with the AUS. These include adjustable and fixed male slings (MS), bulking agents, non-circumferential compression devices such as the ProAct ® [3] and even stem cell therapy [4] . From the current variety of treatment options for male SUI and the mainly poor-quality evidence for device-selection arise a need for clear orientation in comparing these options [2] .…”

Section: Introductionmentioning

confidence: 99%

Risk Factors for Failure of Male Slings and Artificial Urinary Sphincters: Results from a Large Middle European Cohort Study

Hüsch

Kretschmer²,

Thomsen³

et al. 2016

Urol Int

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Introduction: We analysed the impact of predefined risk factors: age, diabetes, history of pelvic irradiation, prior surgery for stress urinary incontinence (SUI), prior urethral stricture, additional procedure during SUI surgery, duration of incontinence, ASA-classification and cause for incontinence on failure and complications in male SUI surgery. Materials and Methods: We retrospectively identified 506 patients with an artificial urinary sphincter (AUS) and 513 patients with a male sling (MS) in a multicenter cohort study. Complication rates were correlated to the risk factors in univariate analysis. Subsequently, a multivariate logistic regression adjusted to the risk factors was performed. A p value <0.05 was considered statistically significant. Results: A history of pelvic irradiation was an independent risk factor for explantation in AUS (p < 0.001) and MS (p = 0.018). Moreover, prior urethral stricture (p = 0.036) and higher ASA-classification (p = 0.039) were positively correlated with explantation in univariate analysis for AUS. Urethral erosion was correlated with prior urethral stricture (p < 0.001) and a history of pelvic irradiation (p < 0.001) in AUS. Furthermore, infection was correlated with additional procedures during SUI surgery in univariate analysis (p = 0.037) in MS. Conclusions: We first identified the correlation of higher ASA-classification and explantation in AUS. Nevertheless, only a few novel risk factors had a significant influence on the failure of MS or AUS.

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“…Thus, since GFs such as IGF, TGF-β, and VEGF present in platelets can stimulate BMSCs proliferation, reduce apoptosis and chromosomal instability, delay the appearance of senescent features, and enhance bone mineralization, the use of PRP in association with BMSCs may help to overcome the limit linked to the efficacy of MSCs therapies [25,[32][33][34][35][36][37]. Additionally, MSCs cultured with PRP were also able to maintain their immune-privileged by modifying cytokine secretion of immune cells (T lymphocytes, B cells, and natural killer cells) to an anti-inflammatory profile and hindered amplification of adaptive immune response [64][65][66]. Finally, besides the stimulating action on MSCs, the GFs released by PRP could also have a direct effect on the cells residing at the defect site [64].…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, MSCs cultured with PRP were also able to maintain their immune-privileged by modifying cytokine secretion of immune cells (T lymphocytes, B cells, and natural killer cells) to an anti-inflammatory profile and hindered amplification of adaptive immune response [64][65][66]. Finally, besides the stimulating action on MSCs, the GFs released by PRP could also have a direct effect on the cells residing at the defect site [64].…”

Section: Discussionmentioning

confidence: 99%

Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant

et al. 2021

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Bone regeneration remains one of the major clinical needs in orthopedics, and advanced and alternative strategies involving bone substitutes, cells, and growth factors (GFs) are mandatory. The purpose of this study was to evaluate whether the association of autologous bone marrow mesenchymal stem cells (BMSC), isolated by ‘one-step surgical procedure’, and activated platelet rich plasma (PRP) improves osseointegration and bone formation of a hydroxyapatite-coated titanium (Ti-HA) implant, already in clinical use, in a rabbit cancellous defect. The GFs present in plasma, in inactivated and activated PRP were also tested. At 2 weeks, histology and histomorphometry highlighted increased bone-to-implant contact (BIC) in Ti-HA combined with BMSC and PRP in comparison to Ti-HA alone and Ti-HA + PRP. The combined effect of BMSC and PRP peaked at 4 weeks where the BIC value was higher than all other treatments. At both experimental times, newly formed bone (Trabecular Bone Volume, BV/TV) in all tested treatments showed increased values in comparison to Ti-HA alone. At 4 weeks Ti-HA + PRP + BMSC showed the highest BV/TV and the highest osteoblasts number; additionally, a higher osteoid surface and bone formation rate were found in Ti-HA + BMSC + PRP than in all other treatments. Finally, the analyses of GFs revealed higher values in the activated PRP in comparison to plasma and to non-activated PRP. The study suggests that the combination of autologous activated PRP, as a carrier for BMSCs, is a promising regenerative strategy for bone formation, osseointegration, and mineralization of bone implants.

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Stem Cell Therapy: Current Applications and Potential for Urology

Cited by 9 publications

References 93 publications

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

Autophagy Improves the Immunosuppression of CD4+ T Cells by Mesenchymal Stem Cells Through Transforming Growth Factor-β1

Risk Factors for Failure of Male Slings and Artificial Urinary Sphincters: Results from a Large Middle European Cohort Study

Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant

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