Stem cell-based tissue engineering with silk biomaterials

Wang, Yongzhong; Kim, Hyeon-Joo; Vunjak-Novakovic, Gordana; Kaplan, David L.

doi:10.1016/j.biomaterials.2006.07.008

Cited by 869 publications

(649 citation statements)

References 259 publications

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“…When the stabilization data presented here are combined with the remarkable mechanical features and tunable release kinetics characteristic of silk carriers (30,43,44), a robust stabilization and delivery platform for antibiotics can be envisioned, extending even to microneedle formats (30). In total, these findings suggest a new path towards eliminating the cold chain, providing new venues towards improved processing, distribution and use of labile therapeutics such as antibiotics and vaccines.…”

Section: Discussionmentioning

confidence: 83%

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Zhang

Pritchard

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Sensitive biological compounds, such as vaccines and antibiotics, traditionally require a time-dependent “cold chain” to maximize therapeutic activity. This flawed process results in billions of dollars worth of viable drug loss during shipping and storage, and severely limits distribution to developing nations with limited infrastructure. To address these major limitations, we demonstrate self-standing silk protein biomaterial matrices capable of stabilizing labile vaccines and antibiotics, even at temperatures up to 60 °C over more than 6 months. Initial insight into the mechanistic basis for these findings is provided. Importantly, these findings suggest a transformative approach to the cold chain to revolutionize the way many labile therapeutic drugs are stored and utilized throughout the world.

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Section: Discussionmentioning

confidence: 83%

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Zhang

Pritchard

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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151

147

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“…The ability of Spidrex to promote a more tenocytic morphology and support tenocyte cell growth is perhaps due to the fact that non-mulberry silks, such as those in Spidrex, contain the cell binding RGD tripeptide motif (Arg-GlyAsp) [39][40][41] and are, therefore, more tenocyte compatible, compared with the other materials tested in this study. Both the qualitative and quantitative cytocompatibility results suggest that Spidrex would pass the in vitro cytotoxicity tests required for medical implant regulatory bodies.…”

Section: Figmentioning

confidence: 97%

“…[32][33][34] Studies evaluating silk as a biomaterial for tendon regeneration have largely focused on the mulberry B. mori silk or composites of B. mori silk with either synthetic polymers or collagen. 28,29,[35][36][37][38] However, given that non-mulberry silks contain the cell binding RGD tripeptide motif, [39][40][41] and have been shown to support fibroblast-like and bone marrow-derived mesenchymal stem cell growth in vitro, 42,43 non-mulberry silk-derived scaffolds hold much promise as biomaterials for enhancing tendon tissue regeneration. 44 In this study, in vitro assays were used to assess the cytocompatibility and immunogenicity of a novel knitted, nonmulberry silk fibroin scaffold designed for use in tendon tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

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In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

Musson

Naot

Chhana

et al. 2015

Tissue Engineering Part A

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Tearing of the rotator cuff tendon in the shoulder is a significant clinical problem, with large/full-thickness tears present in *22% of the general population and recurrent tear rates postarthroscopic repair being quoted as high as 94%. Tissue-engineered biomaterials are increasingly being investigated as a means to augment rotator cuff repairs, with the aim of inducing host cell responses to increase tendon tissue regeneration. Silk-derived materials are of particular interest due to the high availability, mechanical strength, and biocompatibility of silks. In this study, Spidrex Ò , a novel knitted, non-mulberry silk fibroin scaffold was evaluated in vitro for its potential to improve tendon regeneration. Spidrex was compared with a knitted Bombyx mori silk scaffold, a 3D collagen gel and Fiberwire Ò suture material. Primary human and rat tenocytes successfully adhered to Spidrex and significantly increased in number over a 14 day period ( p < 0.05), as demonstrated by fluorescent calcein-AM staining and alamarBlue Ò assays. A similar growth pattern was observed with human tenocytes cultured on the B. mori scaffold. Morphologically, human tenocytes elongated along the silk fibers of Spidrex, assuming a tenocytic cell shape, and were less circular with a higher aspect ratio compared with human tenocytes cultured on the B. mori silk scaffold and within the collagen gel ( p < 0.05). Gene expression analysis by real-time PCR showed that rat tenocytes cultured on Spidrex had increased expression of tenocyte-related genes such as fibromodullin, scleraxis, and tenomodulin ( p < 0.05). Expression of genes that indicate transdifferentiation toward a chondrocytic or osteoblastic lineage were significantly lower in tenocytes cultured on Spidrex in comparison to the collagen gel ( p < 0.05). Immunogenicity assessment by the maturation of and cytokine release from primary human dendritic cells demonstrated that Spidrex enhanced dendritic cell maturation in a similar manner to the clinically used suture material Fiberwire, and significantly upregulated the release of proinflammatory cytokines ( p < 0.05). This suggests that Spidrex may induce an early immune response postimplantation. While further work is required to determine what effect this immune response has on the tendon healing process, our in vitro data suggests that Spidrex may have the cytocompatibility and bioactivity required to support tendon regeneration in vivo.

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“…El rechazo ocasional es producto de la contaminación de la fibroína con sericina generado por errores en el proceso de desgomado [17]. Presenta una gran resistencia mecánica a tracción, junto a una gran flexibilidad, es biodegradable, pero a un ritmo más lento que en otros biopolímeros orgánicos, lo que permite una mejor consolidación de los tejidos.…”

Section: Introductionunclassified

Electrospinning de fibras submicrométricas de Fibroína de seda con Sangre de drago (Croton Lechleri Müll) para la producción de apósitos

Melo

Núñez

Vizuete

et al. 2018

CCTESPE

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Resumen-El presente trabajo de investigación hace uso de la técnica de electrospinning. Los biomateriales utilizados fueron fibroína de seda que resalta entre otros biopolímeros por sus excelentes propiedades mecánicas y biológicas dopado con sangre de drago, savia natural con muchas bondades curativas utilizadas ancestralmente por pueblos indígenas de la Amazonía. Tras la extracción del biopolímero y la preparación de la solución acuosa usando óxido de polietileno (PEO) I. INTRODUCCIÓNLa técnica de electrospinning permite la formación de fibras poliméricas a escala nano y micrométricas mediante el uso fuerzas electrostáticas, los mismos que presentan características particulares entre las que resaltan: alta relación área/volumen, flexibilidad, alta porosidad y un rendimiento mecánico relativamente superior comparados con otros tipos de material de características morfológicas similares; así como la posibilidad de combinar las propiedades de varios polímeros para la formación de fibras [1].Las fibras obtenidas con el tiempo han encontrado cabida en una serie de aplicaciones biomédicas antes desconocidas entre las cuales resaltan: andamios de tejidos [2], apósitos [3]. Los apósitos o wound dressing son elementos que ayudan a evitar la infección y mantener un entorno apropiado que permita la correcta cicatrización de la herida [4] para ello debe contar con ciertas características esenciales como la capacidad hemostática, que son los mecanismos que detienen las hemorragias, poder antibacteriano, absorción de excesos de líquidos exudados como fluidos de la herida o pus, transmisión apropiada entre agua y vapor, capacidad de ajustarse al contorno de la herida, adhesión funcional, es decir, que se adhiera al tejido sano, no al herido, indoloro para el paciente, capacidad de removerse con facilidad y finalmente que sea de bajo costo [5]-[8], esto fortalecido además por beneficios propios de la técnica como la facilidad de trabajar con una serie de polímeros biocompatibles, alta permeabilidad al oxígeno, tamaño de poro variable, la posibilidad de incorporar una variedad de moléculas bioactivas (fármacos como antimicrobianos, antiinflamatorios) en su estructura [9], [10] los pequeños diámetros de fibra, que se asemejan a la matriz extracelular (EM) y al área de superficie grande de una fibra que permite unir las proteínas y los receptores de la membrana celular durante el proceso de cultivo celular. Por lo tanto, son capaces de potenciar la proliferación celular y la formación de fibroplastos [11]- [13].La fibroína de seda es el mayor constituyente proteico en los capullos del gusano de seda (alrededor del 72-81%). En la actualidad se usa ampliamente como biomaterial en el campo biomédico, especialmente en ingeniería de tejidos y en medicina regenerativa, dado que facilita la adhesión de las células, estimula su crecimiento y permite la diferenciación, además, es biocompatible, resistente y biodegradable en fases controlables. [14], [15].Además, presenta una serie de propiedades que la hacen muy interesante como biopol...

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Stem cell-based tissue engineering with silk biomaterials

Cited by 869 publications

References 259 publications

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

Stabilization of vaccines and antibiotics in silk and eliminating the cold chain

In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

Electrospinning de fibras submicrométricas de Fibroína de seda con Sangre de drago (Croton Lechleri Müll) para la producción de apósitos

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