Stem Blight (Phoma Macdonaldii Boerema) in Sunflower Seeds

Saukova, S.L.; Araslanova, N.M.; Antonova, T.S.; Iwebor, Maria

doi:10.25230/2412-608x-2018-2-174-107-111

Cited by 1 publication

(1 citation statement)

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“…In Australia (Chandrashekar, 1993) and China (Wu et al, 2012) the Phoma stem canker causal agent, P. lindquistii, is of quarantine significance. In Russia this fungus is widespread in all sunflower producing regions, such as Krasnodar territory (Borodin, Kotlyarova 2006;Saukova et al, 2014), Tambov province (Vypritskaya et al, 2010), Volgograd province (Saukova et al, 2018), Belgorod province, Central Black Earth region, and North Caucasus (Yakutkin, 2001;2005). Under favorable conditions the fungus can lead to yield losses up to 70 %.…”

Section: Introductionmentioning

confidence: 99%

Identification of Sunflower Pathogenic Fungus Plenodomus Lindquistii Using PCR With Species-Specific Oligonucleotide Primers

Гомжина

Gannibal

2020

1727-1320

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Plenodomus lindquistii causes Phoma black stem of sunflower which is the most common stem disease of this crop in Russia. The diagnostics of both field specimens and pure cultures of P. lindquistii is troublesome. Molecular methods involving the use of the PCR are rapid diagnostic express tests that can precisely identify and detect fungal species. The aim of this study was to develop species-specific oligonucleotide primers for selective amplification of P. lindquistii DNA. The primers LepliF2/LepliR2 were designed on the basis of ITS region analysis and showed stable amplification of the target fungus DNA with no cross-reaction with other fungal species. The primers are recommended for express detection of the causative agent of Phoma black stem of sunflower. This is the first PCR assay that could be used to rapidly reveal and identify this pathogen.

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Section: Introductionmentioning

confidence: 99%