Statistical optimization of Bacillus alcalophilus α-amylase immobilization on iron-oxide magnetic nanoparticles

Nanomaterials as novel supports for the immobilization of amylolytic enzymes and their applications: A review

“…Mukherjee and coworkers [34] reported statistical optimization of immobilized Bacillus alcalophilus alkaline α-amylase onto nano-sized supermagnetic iron oxide MNPs for augmenting the cost effective industrial application of MNP-bound α-amylase. Both Plackett-Burman factorial design and response surface methodology (RSM) were employed to screen the influence of different parameters and the central effect of response on the α-amylaseiron oxide MNP binding process.…”

Optimization for production of liquid nitrogen fertilizer from the degradation of chicken feather by iron-oxide (Fe3O4) magnetic nanoparticles coupled β-keratinase

“…The preparation of iron oxide MNPs, binding of β-keratinase onto the MNPs and characterization of β-keratinase-bound MNPs have been described prior Mukherjee et al, 2010). Evaluation of chicken-feather hydrolysis by either free or iron-oxide MNP-bound keratinase was performed by using 2% (w/ v) washed, dried and finely cut chicken feathers suspended in 100 mM Tris-HCl buffer pH 9.0.…”

“…All these properties imply that magnetic nanoparticles are the best support for the enzyme immobilization process (Ren et al, 2011). In the present study, we used our previously published method for alkarnase (keratinase) immobilization on magnetic nanoparticles Mukherjee et al, 2010). The binding of the magnetic particles to bioactive substances involves a number of interactions including those between the organic ligand, and the interactions between the amino acid side chains of the protein and metal centers (Rodrigues et al, 2011).…”

Effects of surface modification and activation of magnetic nanoparticles on the formation of amylase immobilization bonds under different ionic strength conditions

“…Since, the amino groups existing on the surface of nanoparticles provide a suitable environment for surface treatment through crosslinking with enzyme, the aminated MNPs are activated with GA molecules before immobilization [18,19]. Despite these large number of studies, lack of investigations on the effect of silica coating and density of amino groups on the amount and activity of immobilized enzyme at different ionic strength conditions can be noted.…”