Statistical Optimization of Alkaline Protease Production Using Isolated Strain by Submerged Fermentation

Polley, Tapasi; Ghosh, Uma

doi:10.9734/jabb/2016/27404

Cited by 4 publications

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“…Where Y is the predicted value, β0 is the constant, A is the substrate amount (gm), B is initial moisture (%), C is sucrose (gm) and D is tryptone (gm). The polynomial equation is generated according to the standard method (10).…”

Section: Statistical Analysis and Modelingmentioning

confidence: 99%

Optimisation of fungal glucoamylase production by Response Surface Methodology and characterisation of the purified enzyme

Polley¹,

Ghosh

2020

Plant Sci. Today

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A statistical approach was made for the production of glucoamylase by Aspergillus niger using wheat bran and green gram as fermentation medium. Box-Behnken design used to analyse the simultaneous impact of the substrate, hydration, inoculum volume, fermentation time and temperature, using a second-order polynomial. The experimental results were in good agreement with the proposed regression model with R2 = 0.9322 (p<0.05). The maximum yield (313 U/gds against the predicted value of 306 U/gds) was obtained using 12 gm substrate, 55% moisture, 1 gm sucrose and 0.05 gm tryptone. The purification and characterisation of the enzyme were also studied. Optimum thermal and pH stability was 60 °C and 5 respectively.

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Section: Statistical Analysis and Modelingmentioning

confidence: 99%

Optimisation of fungal glucoamylase production by Response Surface Methodology and characterisation of the purified enzyme

Polley¹,

Ghosh

2020

Plant Sci. Today

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“…A loop full of culture was added into 50 ml of modified basal medium (pH 9.0) containing glucose 30%, casein 1%, KCl 0.5%, FeSO 4 0.01%, MgSO 4 0.5 %, K 2 HPO 4 1% into 250 ml Erlenmeyer flask. The medium was incubated at 30 0 C for 7 days at 120 rpm [9]. At the end of fermentation period, the culture medium was centrifuged at 4000 rpm for 10 minutes and the culture supernatant was used as a crude enzyme.…”

Section: Optimization Of Protease Productionmentioning

confidence: 99%

Optimization of Process Parameters for Production of Alkaline Protease by OVAT Method Using Isolated Strain Alternaria alternata TUSGF1

Polley¹,

Ghosh²

2019

AFSJ

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Aim: The current study aimed at studying the optimum fermentation conditions and nutritional conditions for alkaline protease production by submerge fermentation using Alternaria alternata TUSGF1, isolated from poultry farm soil. Study Design: The results of environmental and nutritional parameters for protease production by OVAT method was analyzed by origin 6.1 software. Place and Duration of Study: Department of Food technology and Biochemical Engineering, Jadavpur University, Kolkata, West Bengal, India between March 2017 and May 2017. Methodology: A protease producing microorganism was isolated from a poultry farm soil and identified as Alternaria alternata TUSGF1. Various environmental and nutritional process parameters such as volume of medium, fermentation time, temperature, age of inoculums, agitation and carbon sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Results: The optimum conditions of protease activity was 30°C at volume of medium 60 ml with 7 days age of inoculum in the medium containing 168 h of incubation and 120 rpm agitation rate. Peptone, casien, skimmed milk, urea and yeast extract were good nitrogen sources whilst maltose, fructose, starch, and sucrose were appropriate for enzyme production by submerge fermentation. Conclusion: Alkaline protease production by a newly isolated Alternaria alternata TUSGF1 from poultry farm soil was studied in shake flask conditions by submerge fermentation. It was established that the optimum protease production was recorded at 30°C, 60 ml volume of medium leaves and incubation time of 168 h. The best carbon and nitrogen sources for protease production were fructose and casein, respectively.

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Application of Used Tea as Solid Matrix for Immobilization of Alkaline Protease by OVAT Method

Polley

Ghosh

2020

Lecture Notes in Bioengineering

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Statistical Optimization of Alkaline Protease Production Using Isolated Strain by Submerged Fermentation

Cited by 4 publications

References 0 publications

Optimisation of fungal glucoamylase production by Response Surface Methodology and characterisation of the purified enzyme

Optimisation of fungal glucoamylase production by Response Surface Methodology and characterisation of the purified enzyme

Optimization of Process Parameters for Production of Alkaline Protease by OVAT Method Using Isolated Strain Alternaria alternata TUSGF1

Application of Used Tea as Solid Matrix for Immobilization of Alkaline Protease by OVAT Method

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