Statistical aspects of quantitative image analysis of β-amyloid in the APPV717F transgenic mouse model of Alzheimer's disease

Plaque‐associated disruption of CSF and plasma amyloid‐β (Aβ) equilibrium in a mouse model of Alzheimer's disease

“…2001) with the percentage Aβ load ranging from no deposition to high levels of deposition. Variability in Aβ deposition between PDAPP mice around this age has been reported by our group (Fishman et al . 2001) and others (Weiner et al .…”

Anti-Amyloid Effects of Small Molecule Aβ-Binding Agents in PS1/APP Mice

“…Grayscale digital images acquired at 10× magnification with a Sony CCD video camera, and montages of the entire surface of cerebral cortex and hippocampus in each tissue section were constructed using Adobe Photoshop 6.0 (Adobe Systems Inc., San Jose, CA). Percent plaque load in the cerebral cortex and hippocampus for each image were determined by standardized region of interest grayscale thresholding analysis [26] using NIH image, and modified algorithms to automate the procedure. Data was expressed as percent plaque load, corresponding to the total amount of area covered with plaques relative to the total brain area.…”

High-throughput quantification of Alzheimer’s disease pathological markers in the post-mortem human brain

“…An approximately normal frequency distribution of cortical ribbon lengths was observed within each cohort of data analysed. This signifies that the location at which sections were cut from tissue blocks was a non-biased selection (Fishman et al, 2001). Furthermore, the broad spread of these measurements is indicative of the high interindividual variability intrinsic to studies on post-mortem human brain.…”

“…In a transgenic mouse model of cerebral ␤-amyloidosis, the variability in plaque burden between mice made a more substantial contribution to the error of the data than either the number of sections analysed from each animal or the percentage of tissue quantified in each section (Fishman et al, 2001). Human brains are intrinsically even more dissimilar than those acquired from a fraternal colony of mice raised in a controlled environment.…”

“…Human brains are intrinsically even more dissimilar than those acquired from a fraternal colony of mice raised in a controlled environment. Within this paradigm, our sampling strategy attempted to optimise the precision of the quantitative data by maximising the number of cases examined (17 cases) as well as to economise on labour and tissue use by minimising the number of sections (1 per case) and sampling fields (5 per section or 20% total) assessed in each case (Fishman et al, 2001).…”