Statistical Analysis of Comet Assay Data

Lovell, David P.

doi:10.1039/9781782622895-00551

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…For tail length values it was done by log 10 transformation. Concerning the tail intensity values, not to get negatives, prior to log 10 transformation, the value of 1 was summed to them, as suggested by Lovell (2017). Signi cances of variances of comet assay descriptors between the negative control animals and nor urazon and nor urazon + UV-B treated groups were tested by one-way ANOVA, followed by post-hoc Tukey LSD.…”

Section: Discussionmentioning

confidence: 99%

Does symbiotic relationship between Hydra viridissima and photoautotrophic alga provide evolutionary advantage in securing the DNA against damage by cytotoxic or genotoxic mode of action of environmental stressors?

Želježić

Kovačev

Matijević

et al. 2023

Preprint

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Symbiosis is an evolutionary strategy that provides biological advantages. The most complex cooperation between symbiotic organisms is endosymbiosis, as it is the symbiosis between green hydra (Hydra viridissima Pallas, 1766) and unicellular photoautotrophic alga. We aimed to evaluate whether this symbiotic cooperation bestows higher resistance to stressors, in terms of preservation of DNA integrity, compared to free-living brown hydra (Hydra oligactis Pallas, 1766). Hydras were exposed to herbicide norflurazon at 2×10− 7 or 2×10− 6 mol/L and UV-B light of 254 nm, 0.023 mW/cm separately or simultaneously. By alkaline comet assay the level of primary DNA damage and by fluorescent staining apoptosis and necrosis were determined. Norflurazon at 2×10− 6 mol/L significantly increased comet assay descriptors in brown hydra compared to negative control (6.17 ± 0.6µm and 5.2 ± 1.7% vs. 2.9 ± 0.2µm and 1.2 ± 0.2%). The frequency of apoptotic and necrotic cells was significantly elevated either, being higher in brown hydra (25.7 ± 3.5% and 8.2 ± 0.2%) than green hydra (20.3 ± 2.5% and 2.8 ± 0.2%). UV-B irradiation induced significant DNA damage in brown hydra only (13.5 ± 1.0µm, 4.1 ± 1.0%). Simultaneous exposure to UV-B light and norflurazon led to synergistic increase in intensity of adverse effects. Norflurazon at 2×10− 7 mol/L combined with UV-B light induced significantly increased DNA lesion-level in brown hydra (15.8 ± 3.1%) and at concentration of 2×10− 6 mol/L in both species (brown: 15.0 ± 2.6%, green: 22.0 ± 3.0%). Cytotoxicity and increased hedgehog nucleoids frequency were recorded, significantly more pronounced in brown (78.3 ± 9.4% and 56.4 ± 6.0%) compared to green hydra (34.7 ± 2.5% and 24.2 ± 0.6%). We proved that evolutionary established symbiotic cooperation may contribute to higher resistance to cyto/genotoxic stressors.

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Section: Discussionmentioning

confidence: 99%

Does symbiotic relationship between Hydra viridissima and photoautotrophic alga provide evolutionary advantage in securing the DNA against damage by cytotoxic or genotoxic mode of action of environmental stressors?

Želježić

Kovačev

Matijević

et al. 2023

Preprint

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“…This parameter was calculated based on fluorescence intensity of stained DNA of each particular spermatozoon and describes percentage of DNA in comet tails (DNA pulled away from cell by electrostatic forces) when compared to total DNA content of comet, hence it positively linearly correlates with DNA damage (Collins et al. ; Lovell, ).…”

Section: Methodsmentioning

confidence: 99%

Influence of photoreactivation on induction of gynogenesis in sterlet,Acipenser ruthenus

Lebeda

Flajšhans

2014

Aquac Res

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“…The slides were placed under a fluorescence microscope (Olympus BX50, Hamburg, Germany) at 10× magnification to take pictures of the stained slide. For image analysis using the Comet software (casplab, comet score, Comet Assay IV), the results of the comet assay were calculated by using Comet scoring software [46].…”

Section: Quantification and Visualization Of Dna Damagementioning

confidence: 99%

Genotoxicity of Synthetic Food Colors on Nitrogen-Fixing Bacteria in Agricultural Lands Irrigated with Wastewater of Corresponding Industries

et al. 2023

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Food colors are considered the most important component of foodstuff for enhancing the aesthetic appeal of the products. The rapid increase in population raised the demand for food materials, while wastewater from as-related processing industries is used for irrigation. This study was conducted to examine the genotoxicity of industrial wastewater on the plant growth-promoting rhizobacteria (PGPR). Three predominantly used synthetic food colors, including Azorubine E-122, Tartrazine E-102 and Allura Red AC E-129, were used during this project. Rhizobacteria were isolated from agricultural soils and treated with various concentrations of Azorubine E-122, Tartrazine E-102 and Allura red E-129 for a 24 and 48 h duration. DNA extraction and quantification were performed through a modified CTAB method, spectrophotometry and agarose gel electrophoresis. A comet assay was used to check DNA damage. According to the results, all the food colors had caused significant damage to DNA depending upon the concentration and exposure time. The extent of DNA damage caused by Azorubine E-122 was relatively greater compared with the other colors, so the fragmentation rate of 86% and 92% was obtained at 1.25% concentration for 24 and 48 h, respectively. The current results have revealed the devastation capacity of food colors by accelerating distortion risk to soil micro-flora, hence the fertility of the soil.

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Statistical Analysis of Comet Assay Data

Cited by 5 publications

References 58 publications

Does symbiotic relationship between Hydra viridissima and photoautotrophic alga provide evolutionary advantage in securing the DNA against damage by cytotoxic or genotoxic mode of action of environmental stressors?

Does symbiotic relationship between Hydra viridissima and photoautotrophic alga provide evolutionary advantage in securing the DNA against damage by cytotoxic or genotoxic mode of action of environmental stressors?

Influence of photoreactivation on induction of gynogenesis in sterlet,Acipenser ruthenus

Genotoxicity of Synthetic Food Colors on Nitrogen-Fixing Bacteria in Agricultural Lands Irrigated with Wastewater of Corresponding Industries

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