State of the art in developmental toxicity screening methods and a way forward: a meeting report addressing embryonic stem cells, whole embryo culture, and zebrafish

Chapin, Robert E.; Augustine-Rauch, Karen; Beyer, Bruce K.; Daston, George P.; Finnell, Richard H.; Flynn, Tom; Hunter, Sidney; Mirkes, Phillip; O’Shea, K. Sue; Piersma, Aldert H.; Sandler, David; Vanparys, Philippe; Maele-Fabry, Geneviève Van

doi:10.1002/bdrb.20158

Cited by 68 publications

(29 citation statements)

References 21 publications

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“…Thus embryonic stem cells themselves model the very young embryo, while embryonic stem cells, which differentiate, model later stages of prenatal development. The most thoroughly developed model based on embryonic stem cells and the only one that has been validated is the "embryonic stem cell test" developed in Europe (Chapin et al, 2008;Croxatto & Villalón, 1994;Dávila et al, 2004). While originally based on differentiation of contracting cardiomyocytes, this assay has undergone continual improvements to include molecular endpoints (Seiler et al, 2004;zur Nieden, Kempka & Ahr, 2004) and also to reduce the time required to perform the assay (Buesen et al, 2009).…”

Section: Studies Using Embryonic Stem Cells (Esc)mentioning

confidence: 99%

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

Talbot

Lin

2011

Biol. Res.

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Numerous studies have repeatedly shown that women who smoke experience problems establishing and maintaining pregnancies, and recent work has further demonstrated that the in utero effects of smoke may not be manifested until months or even years after birth. The purpose of this review is to examine the recent literature dealing with the effects of cigarette smoke on the earliest stages of human prenatal development. Studies in this area have included the use of animal models, patients undergoing in vitro fertilization, and embryonic stem cell models. Events leading to fertilization, such as cumulus expansion, hyperactivation of sperm motility, and oocyte pick-up by the oviduct are all impaired by smoke exposure in animal models. Steps crucial to fertilization such as the acrosome reaction and sperm binding to the zona pellucida are likewise inhibited by cigarette smoke. Preimplantation embryos and stem cells that model embryos show a number of adverse responses to smoke exposure, including poor adhesion to extracellular matrices, diminished survival and proliferation, and increased apoptosis. The current literature demonstrates that the earliest stages of prenatal development are sensitive to smoke exposure and indicates that pregnant women should be advised not to smoke during this time.

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Section: Studies Using Embryonic Stem Cells (Esc)mentioning

confidence: 99%

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

Talbot

Lin

2011

Biol. Res.

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“…However, modifications to the test continue to be suggested. Various recommended changes can be found in Chapin et al [2008] and MarxStoelting et al [2009]. The recommendations include alterations in the evaluation of the endpoint as well as inclusion of other cell lineages.…”

Section: Mouse Embryonic Stem Cell Test (Mest)mentioning

confidence: 99%

Alternative models in developmental toxicology

Lee¹,

Inselman²,

Kanungo

et al. 2012

Systems Biology in Reproductive Medicine

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“…92521, USA; Tel: 951-827-3768; Fax: 951-827-4286; E-mail: talbot@ucr.edu non-mammalian species such as the zebrafish [3]. The cost of performing animal studies is very high, requires a large number of animals for reliable data, often requires a significant amount of time to complete, and is based on non-human species that may not respond the same way to a test chemical as humans.…”

Section: Introductionmentioning

confidence: 99%

“…The value of using in vitro models for measuring toxicity of environmental chemicals and drugs has been appreciated for many years and discussed in recent reviews [3][4]. In contrast to laborious in vivo models that require many animals and may not be accurate predictors for humans, in vitro models potentially enable high throughput screening of chemicals and drugs and can be done using human cells, which should be better predictors of human health effects than models based on non-human species.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in vitro studies are generally less expensive and may be conducted more rapidly than experiments in animal models. Numerous in vitro models using both mammalian and non-mammalian sources have been developed over the past 30 years [3]. In general, these have been good predictors of developmental toxicity, although they vary considerably in the time and difficulty required to perform them.…”

Section: Introductionmentioning

confidence: 99%

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Mouse and Human Embryonic Stem Cells: Can They Improve Human Health by Preventing Disease?

Talbot

Lin²

2011

CTMC

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Given the vast number of chemicals that are released into the environment each year, it is imperative that we develop new predictive models to identify toxicants before unavoidable exposure harms the health of humans and other organisms. In vitro models are especially attractive in predictive toxicology as they can greatly reduce assay costs and animal usage while identifying those chemicals that may require further in vivo evaluation. With the derivation of both mouse and human embryonic stem cells, new opportunities have developed that could revolutionize the field of predictive toxicology. Stem cells themselves can be used to model pre-implantation development, or they can be used during or after differentiation model the earliest stages of development. Because embryos and fetuses are usually more sensitive to environmental toxicants than adults, stem cells provide an unique tool for studying the prenatal phase in our life cycle. The embryonic stem cell test (EST), which has been validated for use with mouse ESC (mESC), is an accurate predictor of embryotoxic compounds, particularly those that are highly embryotoxic. Human embryonic stem cells (hESCs), although not yet incorporated into a validated test, are a particularly attractive platform for toxicological testing as they can give us direct information on humans and avoid concerns about species variation in response. This review discusses toxicological studies and strategies that have been used with embryonic stem cells during the past five years and possible directions that could lead to improvements in the development of predictive assays in the future.

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State of the art in developmental toxicity screening methods and a way forward: a meeting report addressing embryonic stem cells, whole embryo culture, and zebrafish

Cited by 68 publications

References 21 publications

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

The effect of cigarette smoke on fertilization and pre-implantation development: assessment using animal models, clinical data, and stem cells

Alternative models in developmental toxicology

Mouse and Human Embryonic Stem Cells: Can They Improve Human Health by Preventing Disease?

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