Staphylococcal scalded skin syndrome in an extremely low‐birth‐weight neonate: Molecular characterization and rapid detection by multiplex and real‐time PCR of methicillin‐resistant <i>Staphylococcus aureus</i>

Da, Shi; Ishii, Shiro; Sato, Takashi; Yamazaki, Hajime; Matsunaga, Masamichi; Higuchi, Wataru; Takano, Tomomi; Yabe, Shizuka; Tanaka, Kenichi; Yamamoto, Tatsuo

doi:10.1111/j.1442-200x.2010.03246.x

Cited by 15 publications

(11 citation statements)

References 24 publications

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“…CA-MRSA strain NN1 (ST30/SCC mec IVc), which was positive for the collagen adhesin gene ( cna ), was isolated from a child with bullous impetigo in the community [25], [26].…”

Section: Methodsmentioning

confidence: 99%

Comparative Genomics and Drug Resistance of a Geographic Variant of ST239 Methicillin-Resistant Staphylococcus aureus Emerged in Russia

et al. 2012

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Two distinct classes of methicillin-resistant Staphylococcus aureus (MRSA) are spreading in hospitals (as hospital-acquired MRSA, HA-MRSA) and in the community (as community-acquired MRSA, CA-MRSA). Multilocus sequence type (ST) 239 MRSA, one of the most worldwide-disseminated lineages, has been noted as a representative HA-MRSA. Here, we isolated ST239 MRSA (spa type 3 [t037] and staphylococcal cassette chromosome mec [SCCmec] type III.1.1.1) and its novel variant with ST239/spa351 (t030)/SCCmecIII.1.1.4 (SCCmecIIIR) not only from hospitals but also from patients with urethritis in the community in Russia. The Russian variant (strain 16K) possessed a hybrid genome consisting of CC8 and CC30, similar to the ST239/spa3/SCCmecIII.1.1.1 HA-MRSA (TW20) genome, but with marked diversity. The 16K′ CC30 section had SCCmecIIIR carrying the dcs-carrying unit (which corresponded to the SCCmecIVc J3 joining region of ST30 CA-MRSA), lacked SCCmercury, and possessed a novel mobile element structure (MES16K) carrying the ccrC-carrying unit (with the recombinase gene ccrC1 allele 3) and drug resistance tranposons. The Russian variant included strains with a high ability to transfer its multiple drug resistance by conjugation; e.g., for strain 16K, the transfer frequency of a chloramphenicol resistance plasmid (p16K-1 with 2.9 kb in size) reached 1.4×10−2, followed by Tn554 conjugative transfer at 3.6×l0−4. The Russian variant, which has been increasing recently, included divergent strains with different plasmid patterns and pulsed field gel electrophoresis profiles. The data demonstrate the alternative nature of ST239 MRSA as CA-MRSA and also as a drug resistance disseminator, and its micro but dynamic evolution in Russia.

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“…CA-MRSA strain NN1 (ST30/SCC mec IVc), which was positive for the collagen adhesin gene ( cna ), was isolated from a child with bullous impetigo in the community [25], [26].…”

Section: Methodsmentioning

confidence: 99%

Comparative Genomics and Drug Resistance of a Geographic Variant of ST239 Methicillin-Resistant Staphylococcus aureus Emerged in Russia

et al. 2012

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“…Diagnosis of SSSS is based on histological and microbiology findings in collaboration with the overall clinical picture (Mueller, 2009) it has been associated with bullous impetigo in older children (Shi et al, 2011) and clinical symptoms can be similar to Steven Johnson syndrome, Kawasaki disease and Scarlet fever.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus Scalded Skin Syndrome in the newborn: A case review

Smith

Sandall

2012

Journal of Neonatal Nursing

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“…SSSS in newborns has been mainly reported in clonal complexes (CC) 121 and 15, carrying eta and/or etb genes [2], but also in other less common genotypes, such as ET B -positive ST91 MRSA and ET A -positive ST2993-t211 (CC8) with and without SCC mec V [11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Rapid containment of nosocomial transmission of a rare community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) clone, responsible for the Staphylococcal Scalded Skin Syndrome (SSSS)

et al. 2017

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BackgroundThe aims of this study were to identify the source and the transmission pathway for a Staphylococcal Scalded Skin Syndrome (SSSS) outbreak in a maternity setting in Italy over 2 months, during 2014; to implement appropriate control measures in order to prevent the epidemic spread within the maternity ward; and to identify the Methicillin-Resistant Staphylococcus aureus (MRSA) epidemic clone.MethodsEpidemiological and microbiological investigations, based on phenotyping and genotyping methods, were performed. All neonates involved in the outbreak underwent clinical and microbiological investigations to detect the cause of illness. Parents and healthcare workers were screened for Staphylococcus aureus to identify asymptomatic carriers.ResultsThe SSSS outbreak was due to the cross-transmission of a rare clone of ST5-CA-MRSA-SCCmecV-spa type t311, exfoliative toxin A-producer, isolated from three neonates, one mother (from her nose and from dermatological lesions due to pre-existing hand eczema) and from a nurse (colonized in her nose by this microorganism). The epidemiological and microbiological investigation confirmed these as two potential carriers.ConclusionsA rapid containment of these infections was obtained only after implementation of robust swabbing of mothers and healthcare workers. The use of molecular methodologies for typing was able to identify all carriers and to trace the transmission.

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Staphylococcal scalded skin syndrome in an extremely low‐birth‐weight neonate: Molecular characterization and rapid detection by multiplex and real‐time PCR of methicillin‐resistant Staphylococcus aureus

Abstract: ETB-positive ST91 MRSA, which was very similar to ST91 biCA-MRSA, was isolated from an ELBW infant with SSSS. The multiplex and real-time PCR assays specifically or quantitatively detected SSSS-associated and emerging pediatric MRSA.

Cited by 15 publications

References 24 publications

Comparative Genomics and Drug Resistance of a Geographic Variant of ST239 Methicillin-Resistant Staphylococcus aureus Emerged in Russia

Comparative Genomics and Drug Resistance of a Geographic Variant of ST239 Methicillin-Resistant Staphylococcus aureus Emerged in Russia

Staphylococcus Scalded Skin Syndrome in the newborn: A case review

Rapid containment of nosocomial transmission of a rare community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) clone, responsible for the Staphylococcal Scalded Skin Syndrome (SSSS)

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