Standardized method for controlled modification of poly (ethylene terephthalate) (PET) crystallinity for assaying PET degrading enzymes

Thomsen, Thore Bach; Hunt, Cameron; Meyer, Anne S.

doi:10.1016/j.mex.2022.101815

Cited by 15 publications

(27 citation statements)

References 19 publications

(34 reference statements)

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“…The surface features exposed by the enzyme treatment were investigated using Scanning electron microcopy using the procedure described previously [28] . The imaging was performed in low vacuum at an acceleration voltage of 5 kV using a large‐field detector (LTD).…”

Section: Methodsmentioning

confidence: 99%

“…[27] Although some PET hydrolases may degrade highly crystalline PET particles (~40 % X C ) at high initial rates, the enzymatic conversion yields reported on such highly crystalline PET particles have been low. [28,29] The high initial rates on such particles are presumably a result of the extremely high surface area of the PET micro particles that excessively exposes the attackable sites of the heterogeneous PET substrate. In addition, the physicochemical features of the exposed surface layer of the PET particles, such as increased mobility, [30] may exacerbate the initial enzymatic degradation rate of such particles.…”

Section: Introductionmentioning

confidence: 99%

“…Although some PET hydrolases may degrade highly crystalline PET particles (∼40 % X C ) at high initial rates, the enzymatic conversion yields reported on such highly crystalline PET particles have been low [28,29] . The high initial rates on such particles are presumably a result of the extremely high surface area of the PET micro particles that excessively exposes the attackable sites of the heterogeneous PET substrate.…”

Section: Introductionmentioning

confidence: 99%

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Rate Response of Poly(Ethylene Terephthalate)‐Hydrolases to Substrate Crystallinity: Basis for Understanding the Lag Phase

et al. 2023

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The rate response of poly(ethylene terephthalate) (PET)-hydrolases to increased substrate crystallinity (X C ) of PET manifests as a rate-lowering effect that varies significantly for different enzymes. Herein, we report the influence of X C on the product release rate of six thermostable PET-hydrolases. All enzyme reactions displayed a distinctive lag phase until measurable product formation occurred. The duration of the lag phase increased with X C . The recently discovered PET-hydrolase PHL7 worked efficiently on "amorphous" PET disks (X C � 10 %), but this enzyme was extremely sensitive to increased X C , whereas the enzymes LCC ICCG , LCC, and DuraPETase had higher tolerance to increases in X C and had activity on PET disks having X C of 24.4 %. Microscopy revealed that the X C -tolerant hydrolases generated smooth and more uniform substrate surface erosion than PHL7 during reaction. Structural and molecular dynamics analysis of the PET-hydrolyzing enzymes disclosed that surface electrostatics and enzyme flexibility may account for the observed differences.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rate Response of Poly(Ethylene Terephthalate)‐Hydrolases to Substrate Crystallinity: Basis for Understanding the Lag Phase

et al. 2023

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“…PET discs were produced, using a standardized assay (Thomsen et al, 2022), over a range of crystallinities in order to test how binding affinity was affected by crystallinity. Amorphous PET discs were incubated dry in an Eppendorf tube at 115 °C for predefined periods of time, before quenching by plunging the tube into ice-water.…”

Section: Modification Of Pet By Thermal Annealingmentioning

confidence: 99%

“…The K d and B max of BaCBM2 binding to PET films of different crystallinities. Discs were thermally annealed using the process described by (Thomsen et al, 2022), and bulk crystallinity measured by DSC. All assays were performed at 20 °C.…”

Section: Determination Of Residues Important For Bacbm2 Bindingmentioning

confidence: 99%

Protein-Plastic Interactions: The Driving Forces Behind the High Affinity of a Carbohydrate-Binding Module for Polyethylene Terephthalate

2022

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Flexible strain sensors with high sensitivity and large monitoring range prepared by biaxially stretching conductive polymer composites with a bilayer structure

Li,

Xiang,

Zhao

et al. 2023

J of Applied Polymer Sci

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In order to prepare a flexible strain sensor with a large monitoring range, high sensitivity and excellent stability, a polyolefin elastomer (POE)/carbon nanotubes (CNTs) conductive polymer composite with a bilayer structure was prepared by biaxially stretching in this work. The results showed that the bilayer structured nanocomposites had better processability and better stability than monolayer CNT/POE nanocomposites during biaxial stretching. The biaxial stretching process could promote the dispersion and planar orientation of CNTs within the polymer, improving the crystallinity of nanocomposites and the sensing performance of strain sensors. The optimal stretching ratios (SRs) of bilayer structured nanocomposites were studied. The 8 wt% CNT/POE‐POE strain sensor with an SR of 2.5 exhibited a high sensitivity (gauge factor/GF = 72604.7 at 900% strain), wide strain range (0%–900%), and stable cycling performance (1000 cycles at 30% strain), showing a broad application prospect in wearable electronic devices. This study provides a valuable method for efficiently manufacturing high performance and low‐cost flexible strain sensors.

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Standardized method for controlled modification of poly (ethylene terephthalate) (PET) crystallinity for assaying PET degrading enzymes

Cited by 15 publications

References 19 publications

Rate Response of Poly(Ethylene Terephthalate)‐Hydrolases to Substrate Crystallinity: Basis for Understanding the Lag Phase

Rate Response of Poly(Ethylene Terephthalate)‐Hydrolases to Substrate Crystallinity: Basis for Understanding the Lag Phase

Protein-Plastic Interactions: The Driving Forces Behind the High Affinity of a Carbohydrate-Binding Module for Polyethylene Terephthalate

Flexible strain sensors with high sensitivity and large monitoring range prepared by biaxially stretching conductive polymer composites with a bilayer structure

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