Standardization of the Lysozyme Test for a Commercially Available Medium

Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears

“…Although tear proteome studies demonstrate there are hundreds of proteins in human tears, only a few are those most highly abundant proteins [12,19]. To date, very few tear protein tests have been applied to clinical practices [30,31]. The clinical applications of tear tests are limited by the minimal tear volume and complex tear components.…”

Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears

“…Although tear proteome studies demonstrate there are hundreds of proteins in human tears, only a few are those most highly abundant proteins [12,19]. To date, very few tear protein tests have been applied to clinical practices [30,31]. The clinical applications of tear tests are limited by the minimal tear volume and complex tear components.…”

Improvement of Sjögren's syndrome after two infusions of rituximab (anti‐CD20)

“…Xerostomia was evaluated using the unstimulated salivary flow rate (5 minutes; samples were weighed and 1 gram corresponded to 1 ml) at 8:00 AM on an empty stomach. Ocular dryness was evaluated by an ophthalmologist (BC) using Schirmer's test (33) and the Van Bijsterveld score (34). These tests were carried out at weeks 0, 12, and 36.…”

Tear Function Parameters in Keratoconjunctivitis sicca with and without the Association of Sjögren's Syndrome

“…The tear fluid lysozyme concentration was deter mined by the agar diffusion method [11]. Hen eggwhite lysozyme (HEL) standards were used to express the lysozyme concentration in micrograms per milli liter HEL equivalent [12].…”

Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears

“…Although tear proteome studies demonstrate there are hundreds of proteins in human tears, only a few are those most highly abundant proteins [12,19]. To date, very few tear protein tests have been applied to clinical practices [30,31]. The clinical applications of tear tests are limited by the minimal tear volume and complex tear components.…”

Improvement of Sjögren's syndrome after two infusions of rituximab (anti‐CD20)

“…Xerostomia was evaluated using the unstimulated salivary flow rate (5 minutes; samples were weighed and 1 gram corresponded to 1 ml) at 8:00 AM on an empty stomach. Ocular dryness was evaluated by an ophthalmologist (BC) using Schirmer's test (33) and the Van Bijsterveld score (34). These tests were carried out at weeks 0, 12, and 36.…”

Tear Function Parameters in Keratoconjunctivitis sicca with and without the Association of Sjögren's Syndrome

“…The tear fluid lysozyme concentration was deter mined by the agar diffusion method [11]. Hen eggwhite lysozyme (HEL) standards were used to express the lysozyme concentration in micrograms per milli liter HEL equivalent [12].…”

Quantification of tear proteins by SDS-PAGE with an internal standard protein: A new method with special reference to small volume tears

“…Although tear proteome studies demonstrate there are hundreds of proteins in human tears, only a few are those most highly abundant proteins [12,19]. To date, very few tear protein tests have been applied to clinical practices [30,31]. The clinical applications of tear tests are limited by the minimal tear volume and complex tear components.…”

Improvement of Sjögren's syndrome after two infusions of rituximab (anti‐CD20)

“…Xerostomia was evaluated using the unstimulated salivary flow rate (5 minutes; samples were weighed and 1 gram corresponded to 1 ml) at 8:00 AM on an empty stomach. Ocular dryness was evaluated by an ophthalmologist (BC) using Schirmer's test (33) and the Van Bijsterveld score (34). These tests were carried out at weeks 0, 12, and 36.…”

Tear Function Parameters in Keratoconjunctivitis sicca with and without the Association of Sjögren's Syndrome

“…The tear fluid lysozyme concentration was deter mined by the agar diffusion method [11]. Hen eggwhite lysozyme (HEL) standards were used to express the lysozyme concentration in micrograms per milli liter HEL equivalent [12].…”