Standardization of seed storage conditions in chilgoza pine (Pinus gerardiana Wall.): an endangered pine of Hind Kush Himalaya

Malik, A. R.; Shamet, G. S.; Butola, Jitendra S.; Bhat, G. M.; Mir, A. A.; Nabi, Gowher

doi:10.1007/s00468-013-0868-y

Cited by 7 publications

(6 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various seed storage temperatures have been reported for P. nigra, which have resulted in the ability to store the seeds for different lengths of time and still retain sufficient levels of seed viability (Stanton and Villar 1996;Tylkowski 2010;Michalak et al 2014;Suszka et al 2014). Thus far in the literature, optimal seed storage conditions have been assessed on the basis of seed germination (Popova et al 2012;Malik et al 2013;Suszka et al 2014) or seedling emergence (Michalak et al 2013;Popova et al 2013). These parameters do measure the impact of storage temperature on seed viability and initial emergence.…”

Section: Introductionmentioning

confidence: 99%

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

View full text Add to dashboard Cite

Key message Seed storage temperature influences root anatomy of the endangered Populus nigra, and consequently may alter nutrient absorption. A lower temperature during seed storage (−20 and −196 °C) may preserve the potential for a suitable root system development after germination. Context Seed storage conditions can be an important determinant of later seedling growth of Populus nigra L., an endangered tree species. Aims We tested whether long-term seed storage temperature, −10, −20 or −196 °C, affects the pattern of seedling root traits responsible for resource acquisition as compared to seedlings of fresh seeds. Methods We analysed the morphology, anatomy, degree of mycorrhizal colonization, and biochemical composition of roots developed from seed stored for 24 months at five different temperatures (from 3 to −196 °C) commonly used to preserve genetic resources. Results Except for root anatomy, we found no relationship between seed storage temperature and the root traits of seedlings. Among the various storage conditions, the proportion of roots with primary development in the first four orders was similar in seedlings developed from fresh seeds of from seeds stored at −196 or −20 °C. Nitrogen content in the roots was positively correlated with the proportion of (i) roots with primary development and (ii) the cortex width in the root diameter. Conclusions Higher temperatures during seed storage reduced the proportion of roots with absorptive function (with primary development). Therefore, for preservation of P. nigra seeds we recommend lower temperatures such as −20 and −196 °C.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Mucha

Szymańska

Zadworny

et al. 2015

Annals of Forest Science

View full text Add to dashboard Cite

show abstract

“…Chilgoza pine or pine nuts play an important role in the socio-economic development of rural societies existing nearby Chilgoza forests [7] (Kumar et al, 2013). Chilgoza pine forests not only provide pine nuts as an economic product but also provide fuelwood, medicinal plants, pasture, and shelter for livestock as well as environment habitat and other ecological services.…”

Section: Economic Importance Of Pinementioning

confidence: 99%

“…The tree bark, wood, leaves, and cones are resinous and produce oleoresins [7] (MAIL, 2012) [13]. Oleoresins can be obtained by tapping tree bark, though; it is not created in large quantities [13].…”

Section: Economic Importance Of Pinementioning

confidence: 99%

Baseline Study on Pine Nuts Industry in Khost City, Afghanistan

Atif

2021

AJAEES

View full text Add to dashboard Cite

This study was conducted on the pine nuts industry in the southeast of Afghanistan. This is a baseline study, which has conducted between May 2019 and December 2020. This study aims to provide essential information about the pine nuts industry, especially focused on the supply chain of pine nuts. A structured questionnaire has been used for the data collection to collect demographic information, and information regarding the pine nuts supply chain, pine nuts processing, challenges and opportunities, trade, and marketing, Due to the small size of the population, the census method was used. I interviewed directly the stakeholder and entrepreneurs who are working in the pine nuts industries; the interview has been conducted on local language (Pashto). Chilgoza plays an important role in the socio-economic development of rural societies existing nearby Chilgoza forests. Chilgoza pine forests not only provide pine nuts as a cash crop product but also provide fuelwood, medicinal plants, pasture, and shelter for livestock as well as environment habitat and other ecological services. Nuts of Chilgoza pine are highly valued, collected, and sold by local villagers is a good source of income for them. After extracting nuts from the cones, the owner, sells the empty cones to the people of Khost province of Afghanistan, usually used as fuelwood.

show abstract

“…(Duan et al 2017). Investigations on Chilgoza pine ecology (Kumar et al 2013), seed germination (Saeed and Thanos 2006), genetic variability of phenotypic traits (Kant et al 2006a), seed protein properties (Cai et al 2013), storage and drying treatments (Malik et al 2013;Thakur et al 2014), and morphometric characters (Ranot and Shrama 2015) have been carried out in other countries, especially in India and Pakistan, and also in Afghanistan (Khurram and Shalizi 2016;Safari et al 2017;Alami and Mousavi 2020). In natural populations of P. gerardiana, high levels of genetic variation at RAPD (Randomly Amplified Polymorphism DNA) markers were found within populations in India (Kant et al 2006b).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and fine-scale spatial genetic structure of the near-threatened Pinus gerardiana in Gardiz, Afghanistan

Moosavi¹,

Budde²,

Mueller³

et al. 2022

plecevo

View full text Add to dashboard Cite

Background and aims – Chilgoza pine (Pinus gerardiana) is a near-threatened tree species from the north-western Himalayas. This species is the economically most important pine in Afghanistan because of its edible nuts; however, its distribution range is disjunct and restricted to a few isolated regions. The IUCN lists Chilgoza as a near threatened species because of overexploitation of its nuts and a declining population trend. This research is the first in-depth analysis of the genetic variability and structure of Chilgoza in Afghanistan using microsatellite markers. Material and methods –We tested cross-amplification of 44 SSR markers developed for pine species. Eight polymorphic EST-SSRs were genotyped in a natural Chilgoza population in Gardiz, Afghanistan. To evaluate the genetic diversity, fine-scale spatial genetic structure (SGS), signatures of bottleneck events, and the effective population size, 191 trees were sampled and genotyped. Based on the diameter at breast height, individuals were classified as young or old trees. Key results – Genetic variation in the whole population was moderate. For individual markers, He ranged from 0.130 to 0.515 (mean = 0.338) and Ho from 0.118 to 0.542 (mean = 0.328). The expected heterozygosity in young trees was slightly lower than in old trees. The SGS was stronger for young trees (Sp = 0.0100) than for old trees (Sp = 0.0029). Heterozygosity excess analysis detected no recent population size reduction, but the M ratio revealed an ancient and prolonged bottleneck in the Chilgoza population. Conclusion – Identification of suitable EST-SSRs for future studies of natural Chilgoza populations provides important tools for the conservation of the species. Despite the moderate genetic variation in Gardiz, scarcity of natural regeneration is likely to reduce the genetic variation and adaptability in future generations. Our results indicated a slight decrease in genetic diversity and stronger SGS in young trees calling for conservation measures fostering natural regeneration.

show abstract

Standardization of seed storage conditions in chilgoza pine (Pinus gerardiana Wall.): an endangered pine of Hind Kush Himalaya

Cited by 7 publications

References 3 publications

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Baseline Study on Pine Nuts Industry in Khost City, Afghanistan

Genetic diversity and fine-scale spatial genetic structure of the near-threatened Pinus gerardiana in Gardiz, Afghanistan

Contact Info

Product

Resources

About