Standardization of Purified Extract Mahoni Seed and Antioxidant Activity

Abstract

Indonesia has many plants that have been used for treating many diseases such as diabetic. One of the Indonesian plants has been shown the antidiabetic activity such as mahoni. In this study, we have investigated the standardization of the purified extract of the mahoni seeds and its antioxidant activity. The standardization methods include organoleptic test, determination of drying loss, determination of, total ash level, determination of acid-insoluble ash level, determination of water-soluble essence level, determination of ethanol soluble essence level, phytochemical profile test: alkaloid test, flavonoid test, phenolic test, saponin test, terpenoid, and steroid test. The results show the purified extract mahoni seed qualify as a raw material for herbal medicine and also has potential as an antioxidant IC 50 33.86 ug/ml that compare with the standard.

Standardization and bacteria inhibitory test of purified extract of mahogany (Swietenia mahagoni (L.) Jacq) seeds and leaves

“…The data above shows that the yield of ethanol extract of seeds of mahogany that is 5.83% and the yield of puri ied extract mahogany seeds as much as 33.79% (Handayani et al, 2019). While the value of the yield of ethanol extract of leaves of mahogany that is 10.67% and the yield of puri ied extract of leaves of mahogany as much as 69.61%.…”

“…Microbial contamination testing that has been done shows that the puri ied extract seeds mahogany (Swietenia mahagoni (L.) Jacq) has a value of ALT bacteria1 x 102 colonies / g and the numerical value of yeast fungi of 4x10 colonies/g, which means it meets the requirements set out in the literature. That the ALT value≤104 bacterial colonies/g and the number of yeastfungi ≤ 103 colonies/g (Handayani et al, 2019).…”

“…This research aims to establish the parameters of standardization of puri ied extract from mahogany (Swietenia mahagoni(L.) Jacq) seeds so that the future can provide scienti ic information to ensure the inal product and its use can be accepted and can be developed as one of the Indonesian native plants that can be used as ingredients for traditional medicines (Handayani et al, 2019).…”

“…According to SK Badan POM Nomor12 about maximum limit metal contamination in food, states that the maximum limit metal contamination of lead (Pb) and cadmium (Cd) on at ≤10 mg / g to metallic lead (Pb), while the metal cadmium by ≤0,3 ug / g (BPOM RI, 2014). From the metal, content measurement showed that the puri ied extract mahogany seeds had higher levels of 0.0607 ug / g to metallic lead (Pb) and <0,003 ug / g for metal cadmium (Cd) (Handayani et al, 2019).…”

“…Prepared 4 test tube, and included 9 mL of diluent to each tube. Pipette in 1 mL of diluent 10-1 into the irst test tube, shaken until homogeneous to obtain a dilution of 10-2, then proceed with the dilution of 10-3, 10-4 and 10-5 (Handayani et al, 2019).…”