Standardization and evaluation of a tetraplex polymerase chain reaction to detect and differentiate Mycobacterium tuberculosis complex and nontuberculous Mycobacteria—a retrospective study on pulmonary TB patients

Anilkumar, A.K.; Madhavilatha, G. K.; Paul, Laiza K.; Radhakrishnan, Indulakshmi; Kumar, Ram Mohan Ram; Mundayoor, Sathish

doi:10.1016/j.diagmicrobio.2011.11.006

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“…DNA was subjected to a tetraplex PCR to differentiate between M. tuberculosis complex and nontuberculous mycobacteria. DNA was subjected to amplification and sequencing of the 3 target regions separately, 16S–23S internal transcribed spacer region, hsp65, and rpoB separately ( 7 ). M. tuberculosis H37Rv and M. bovis bacilli Calmette-Guérin genomic DNA was used as control DNA for the PCR studies.…”

Section: The Studymentioning

confidence: 99%