Staged self-assembly of PAMAM dendrimers into macroscopic aggregates with a microribbon structure similar to that of amelogenin

Yang, Jiaojiao; Cao, Shuqin; Li, Jiahui; Xin, Jianyu; Chen, Xingyu; Wu, Wei; Xu, Fu‐Jian; Li, Jianshu

doi:10.1039/c3sm51510a

Cited by 26 publications

(21 citation statements)

References 44 publications

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“…We have previously demonstrated that alendronate-conjugated PAMAM dendrimer could induce in situ remineralization of tooth enamel, which is due to the hydroxyapatite-anchored property of alendronate [29]. Meanwhile, we also found that the carboxyl-terminated dendrimer, which has similar structure and self-assembly behavior to that of amelogenin [30], could be immobilized within the collagen matrix and induce intrafibrillar mineralization with hierarchical structure on human dentin [31,32]. However, the chemical structures of above materials are not similar to that of natural NCPs in terms of the peripheral groups.…”

Section: Introductionmentioning

confidence: 75%

“…Since the environment of the biomineralization contains a lot of flowing liquid, the binding strength to the dentin is very important for dendrimer to fulfill its function in the remineralization process. In our and other groups' previous reports, the dendrimer with proper molecular weight can be immobilized within collagen matrix via both size-exclusion features (i.e., only proteins with molecular weights between 6 and 40 kDa can diffuse into the collagen hole and be immobilized within the collagen matrix) and electrostatic interaction of the cross-linked collagen which is the premise of mineralization of dentinal tubule [30,38]. In order to characterize the binding capability, both G4-COOH, which has been proved as a successful material for dentin mineralization [30], and G4-PO 3 H 2 were applied on the surfaces of EDTA-etched tooth dentin specimens, followed by washing with 0.1 M PBS solution since PBS buffer can wash off unbound dendrimer [39].…”

Section: Binding Capability Of G4-po 3 H 2 To Demineralized Dentinmentioning

confidence: 98%

“…Previous researchers have demonstrated that EDTA-etching is an excellent approach [30], since the minerals and natural NCPs are found to be removed without causing the denaturation of collagen after EDTA-etching. Thus the EDTA-etching approach was applied in this study.…”

Section: Demineralization Of Edta-etched Dentin Disksmentioning

confidence: 98%

“…Generally, the crystals directly formed by homogeneous deposition of calcium and phosphate ions can only exhibit on the surface or out of the collagen fibrils in the solution [30]. Since the mechanical properties of mineralized collagenous tissues such as dentin are dependent on their intrafibrillar apatite minerals [43], it is important to focus on the mechanism of the intrafibrillar remineralization.…”

Section: Remineralization On Human Dentinmentioning

confidence: 99%

See 3 more Smart Citations

Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein

Zhang

Yang

Liang

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 75%

Section: Binding Capability Of G4-po 3 H 2 To Demineralized Dentinmentioning

confidence: 98%

Section: Demineralization Of Edta-etched Dentin Disksmentioning

confidence: 98%

Section: Remineralization On Human Dentinmentioning

confidence: 99%

See 2 more Smart Citations

Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein

Zhang

Yang

Liang

et al. 2015

Colloids and Surfaces B: Biointerfaces

Self Cite

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“…The self-assembly behavior of PAMAM-COOH was observed by the method reported by Yang et al [2013]. A solution containing 1 mg/ml 4.5G PAMAM-COOH and 10 m M Ca(NO 3 ) 2 was prepared and kept static for 5, 10, and 24 h at 37 ° C. At each scheduled time, 100 μl of the solution was pipetted out for morphological observation of the self-assembly behavior of PAMAM-COOH.…”

Section: Self-assembly Behavior Of Pamam-cooh In Ca 2+ Solutionmentioning

confidence: 99%

Biomimetic Remineralization of Human Enamel in the Presence of Polyamidoamine Dendrimers in vitro

Chen

He²,

Tang³

et al. 2015

Caries Res

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Poly(amidoamine) (PAMAM) dendrimers, known as artificial proteins, have unique and well-defined molecular size and structure. It has previously been used to mimic protein-crystal interaction during biomineralization. In this study, generation 4.5 (4.5G) PAMAM with carboxylic acid (PAMAM-COOH) was synthesized and utilized to remineralize the surface of etched enamel in vitro. Using confocal laser scanning microscopy, Fourier transform infrared spectroscopy, X-ray diffraction analysis and scanning electron microscopy we observed that 4.5G PAMAM-COOH can be absorbed on the etched enamel surface and that it can induce the formation of hydroxyapatite crystals with the same orientation as that of the enamel prisms on longitudinal and transversal enamel surfaces. The self-assembly behavior of PAMAM in the mineralization solution was also investigated and the result showed that 4.5G PAMAM can assemble to microribbon structure similar to the behavior of amelogenins. Therefore, we concluded that 4.5G PAMAM-COOH assemblies can act as the organic template on enamel surface and in mineralization solution to control the nucleation site and morphology of new-grown crystals to form the biomimetic structure of human enamel, which may open a new way for repairing damaged enamel.

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Implicit solvent coarse‐grained model of polyamidoamine dendrimers: Role of generation and pH

2015

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Highly branched polymers such as polyamidoamine (PAMAM) dendrimers are promising macromolecules in the realm of nanobiotechnology due to their high surface coverage of tunable functional groups. Modeling efforts of PAMAM can provide structural and morphological properties, but the inclusion of solvents and the exponential growth of atoms with generations make atomistic simulations computationally expensive. We apply an implicit solvent coarse-grained model, called the Dry Martini force field, to PAMAM dendrimers. The reduced number of particles and the absence of a solvent allow the capture of longer spatiotemporal scales. This study characterizes PAMAM dendrimers of generations one through seven in acidic, neutral, and basic pH environments. Comparison with existing literature, both experimental and theoretical, is done using measurements of the radius of gyration, moment of inertia, radial distributions, and scaling exponents. Additionally, ion coordination distributions are studied to provide insight into the effects of interior and exterior protonation on counter ions. This model serves as a starting point for future designs of larger functionalized dendrimers.

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Staged self-assembly of PAMAM dendrimers into macroscopic aggregates with a microribbon structure similar to that of amelogenin

Cited by 26 publications

References 44 publications

Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein

Effective dentin restorative material based on phosphate-terminated dendrimer as artificial protein

Biomimetic Remineralization of Human Enamel in the Presence of Polyamidoamine Dendrimers in vitro

Implicit solvent coarse‐grained model of polyamidoamine dendrimers: Role of generation and pH

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