Stage-dependent redistributions of acetylated histones in nuclei of the early preimplantation mouse embryo

Dynamics of epigenetic modifications in ICSI embryos from in vitro‐produced spermatozoa

“…The presence of H3K9ac at the 2‐cell stage in in vivo‐fertilized embryos and in ICSI embryos is consistent with the transient enrichment of acetylated histones during EGA 51 . This modified histone distributes throughout the nucleoplasm in advanced stage embryos 51 . The evolution of this histone PTM in embryos derived from in vivo ‐ or in vitro ‐ produced spermatozoa was similar during the embryonic development (Figure 6E).…”

Reconstitution of Enhancer Function in Paternal Pronuclei of One-Cell Mouse Embryos

“…Similarly, endogenous genes are more strongly expressed in the paternal pronuclei of S-phase-arrested one-cell embryos than in either the maternal pronuclei of S-phase-arrested one-cell embryos or the zygotic nuclei of two-cell embryos, most likely because of the lack of chromatin-mediated repression in the paternal pronuclei (4). This pattern of expression of endogenous genes is very similar to that of plasmid-encoded genes microinjected into these types of nuclei (23,30,45,46,51,52). As shown in this work, reconstitution of chromatin-mediated repression on plasmid-borne promoters injected into S-phase-arrested onecell embryos transforms the expression pattern of these promoters into that observed in two-cell embryos, again strengthening this correlation.…”

Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer

“…According to data by Stein et al (Stein et al, 1997), H3K9 and H4K8 are sensitive to histone deacetylase inhibitors whereas H3K14 is nonsensitive. As expected, HSC clone embryos were more strongly stained with the antibodies for H3K9 and H4K8, but not with the antibody for H3K14, as compared with cumulus clone embryos and intracytoplasmic sperm injection (ICSI) embryos (Fig.…”