Stage-dependent effects of cell-to-cell connections on in vitro induced neurogenesis

Tárnok, Krisztián; Pataki, Ágnes; Kovács, János; Schlett, Katalin; Madarász, Emı́lia

doi:10.1078/0171-9335-00262

Cited by 30 publications

(29 citation statements)

References 33 publications

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“…2B and Table 1). If induced by all-trans RA, NE-4C cells develop into neurons and glial cells through well characterized stages of differentiation, including transient states of radial glia like development characterized with Pax6 and Emx2 expression and RC2-immunopositivity [29,33,41,42]. Mature neurons with synapsin expression and specific machinery for neurotransmitter production, release and uptake develop by the 9-10th day after RA induction [31].…”

Section: Resultsmentioning

confidence: 99%

“…For this purpose, neural stem cells cloned from early embryonic (E9) mouse neuroectoderm (NE-4C cells; [29][30][31][32][33][34]) and stem/progenitor cells isolated from late embryonic (E14.5) or adult (P62) neurogenic zones (RGl cells; [35]) were used. The results demonstrated that the investigated stem/ progenitor populations showed significant differences in many aspects of both RA metabolism and retinoid responsiveness.…”

Section: Introductionmentioning

confidence: 99%

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Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Orsolits

Borsy

Madarász

et al. 2013

Stem Cells and Development

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Orsolits

Borsy

Madarász

et al. 2013

Stem Cells and Development

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“…7E, G; Supplemental Movie 2). Aggregate formation was previously shown to be indispensable for the RA-induced neuronal differentiation of NE-4C cells (39,40).…”

Section: Rar Signaling Is Involved In the Early Steps Of Gliainduced mentioning

confidence: 98%

Astroglia‐derived retinoic acid is a key factor in glia‐induced neurogenesis

Környei

Gócza

Rühl³

et al. 2007

FASEB j.

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Astroglial cells are essential components of the neurogenic niches within the central nervous system. Emerging evidence suggests that they are among the key regulators of postnatal neurogenesis. Although astrocytes have been demonstrated to possess the potential to instruct stem cells to adopt a neuronal fate, little is known about the nature of the glia-derived instructive signals. Here we propose that all-trans retinoic acid, one of the most powerful morphogenic molecules regulating neuronal cell fate commitment, may be one of the glia-derived factors directing astroglia-induced neurogenesis. According to data obtained from several complementary approaches, we show that cultured astrocytes express the key enzyme mRNAs of retinoic acid biosynthesis and actively produce all-trans retinoic acid. We show that blockage of retinoic acid signaling by the pan-RAR antagonist AGN193109 prevents glia-induced neuron formation by noncommitted stem cells. Therefore, we provide strong in vitro evidence for retinoic acid action in astroglia-induced neuronal differentiation.

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“…It is known that cell-cell communication is an important prerequisite for differentiation of CNS stem cells via lateral induction / inhibition [24,[72][73][74], a process that is mediated by Notch signalling and involves interaction between signalling and neighboring cells. It is possible, therefore, that adjacent cells initially promote the permanent proliferation of stem cells in order to establish cellcell contacts required for differentiation [29].…”

Section: Discussionmentioning

confidence: 99%

Neural Differentiation of Embryonic Stem Cells is Induced by Signalling from Non-Neural Niche Cells

Bentz¹,

Molcanyi²,

Heß³

et al. 2006

Cell Physiol Biochem

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Background/Aims: Embryonic stem cell (ESC) transplantation offers new therapeutic strategies for neurodegenerative diseases and injury. However, the mechanisms underlying integration and differentiation of engrafted ESCs are poorly understood. This study elucidates the influence of exogenous signals on ESC differentiation using in vitro modelling of nonstem/ stem cell interactions. Methods: Murine ESCs were co-cultured with endothelial cells and astrocytes or conditioned medium obtained from endothelial or astrocyte cultures. After 7 days of co-culture isolated RNA was analysed using RT-PCR for the expression of pluripotency marker oct-4, neural progenitor marker nestin, and neurofilament (NFL), an early marker of neuronal lineage commitment. The presence of the glial cell surface marker A2B5 was determined in ESCs by flow cytometry. Results: Neuronal differentiation was inhibited in ESCs when grown in close vicinity to cerebral endothelial or glial cells.Under these conditions, ESC differentiation was predominantly directed towards a glial fate. However, treatment of ESCs with endothelial cell-or astrocyteconditioned medium promoted neuronal as well as glial differentiation. Conclusion: Our results indicate that ESC fate is determined by endothelial and glial cells that comprise the environmental niche of these stem cells in vivo. The direction of differentiation processes appears to be dependent on humoral factors secreted by adjacent cell lines.

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Stage-dependent effects of cell-to-cell connections on in vitro induced neurogenesis

Cited by 30 publications

References 33 publications

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Retinoid Machinery in Distinct Neural Stem Cell Populations with Different Retinoid Responsiveness

Astroglia‐derived retinoic acid is a key factor in glia‐induced neurogenesis

Neural Differentiation of Embryonic Stem Cells is Induced by Signalling from Non-Neural Niche Cells

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