Stable Laboratory Culture System for the Ctenophore &lt;em&gt;Mnemiopsis leidyi&lt;/em&gt;

Burkhardt, Pawel; Soto-Àngel, Joan Soto; Nordmann, Eva-Lena; Sturm, Daniela; Sachkova, Maria Y.; Pang, Kevin

doi:10.20944/preprints202202.0341.v1

Cited by 3 publications

(3 citation statements)

References 37 publications

(54 reference statements)

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“…As a wider range of modern techniques has recently become available in ctenophores (e.g., single‐cell RNAseq [Sebé‐Pedrós et al, 2018] and connectomics [Burkhardt et al, 2023]) and ctenophore species have progressed towards becoming established lab models (e.g., culturing and CRISPR/Cas9 knock‐outs have been reported [Presnell et al, 2022; Soto‐Àngel et al, 2022]), new critical insights into the biology of their neurons have been revealed. Earlier studies have established such key approaches as electrophysiology and calcium imaging in ctenophores; however, it has been achieved only in comb plates (Moss & Tamm, 1987; Tamm & Terasaki, 1994) and muscles (Moroz et al, 2014; Ortiz et al, 2023), while direct measurements of neuronal activity have not been reported.…”

Section: Discussionmentioning

confidence: 99%

“…Due to the high ecological plasticity of Mnemiopsis leidyi , it has become an important invasive species that colonized a wide range of marine environments and disrupted native ecosystems (Burton, 2008). Such a plasticity along with high regenerative capacity (Edgar et al, 2021) probably explains why M. leidyi has become a lab model organism (Martindale, 2022) that can adapt to the artificial conditions of aquarium facilities and be successfully cultured and reproduced over multiple generations (Presnell et al, 2022; Soto‐Àngel et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Evolutionary origin of the nervous system from Ctenophora prospective

Sachkova

2024

Evolution and Development

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Nervous system is one of the key adaptations underlying the evolutionary success of the majority of animal groups. Ctenophores (or comb jellies) are gelatinous marine invertebrates that were probably the first lineage to diverge from the rest of animals. Due to the key phylogenetic position and multiple unique adaptations, the noncentralized nervous system of comb jellies has been in the center of the debate around the origin of the nervous system in the animal kingdom and whether it happened only once or twice. Here, we discuss the latest findings in ctenophore neuroscience and multiple challenges on the way to build a clear evolutionary picture of the origin of the nervous system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evolutionary origin of the nervous system from Ctenophora prospective

Sachkova

2024

Evolution and Development

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“…The implementation of such non-conventional research models is nowadays essential to gain insight into the mechanisms underlying these complex biological traits at the cellular, molecular, and genetic level Arboleda et al, 2018;Ivankovic et al, 2019;Kassmer et al, 2019;Layden et al, 2016;Lechable et al, 2020;Mehta and Singh, 2019;Röttinger, 2021;Srivastava, 2022). Methods for the development and improvement of the entire life cycle in stable and reproducible laboratory conditions have recently been described for several marine vertebrates (Roux et al, 2020) and invertebrates (Carvalho et al, 2017;Gordon et al, 2020;Henry et al, 2020;Holland and Li, 2021;Lechable et al, 2020;Murabe et al, 2021;Soto-Àngel et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Optimized husbandry and targeted gene-editing for the cnidarianNematostella vectensis

Carvalho

Burtin

Detournay³

et al. 2023

Preprint

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Optimized laboratory conditions for research models are crucial for the success of scientific projects. This includes the control of the entire life cycle, access to all developmental stages and maintaining stable physiological conditions. Reducing the life cycle of a research model can also enhance the access to biological material and speed up genetic tool development. Thus, we optimized the rearing conditions for the sea anemone Nematostella vectensis, a cnidarian research model to study embryonic and post-metamorphic processes, such as regeneration. We adopted a semi-automated aquaculture system for N. vectensis and developed a dietary protocol optimized for the different life stages. Thereby, we increased spawning efficiencies and post-spawning survival rates, and considerably reduced the overall life cycle down to two months. To further improve the obtention of CRISPR-Cas9 mutants, we optimized the design of sgRNAs leading to full KO animals in F0 polyps using a single sgRNA. Finally, we show that NHEJ-mediated transgene insertion is possible in N. vectensis. In sum our study provides additional resources for the scientific community that uses or will use N. vectensis as a research model.

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An effective method to mass culture a lobate ctenophore (<i>Bolinopsis mikado</i>)

Ikeda¹,

Sato²,

Yamaguchi³

et al. 2022

Plankton Benthos Res

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This paper reports an effective and easy method to culture the ctenophore Bolinopsis mikado. Fifteen adult B. mikado individuals were maintained in a kreisel tank, and fertilized eggs, which were detected in the following days, were all raised together in the same tank. From the early development stage to day 13, the B. mikado cydippid larvae were only fed Artemia nauplii. The cydippid larvae were able to easily catch the Artemia nauplii excreted by adult B. mikado; therefore, they were considered capable of growing on excrement. Adult B. mikado individuals were removed from the tank when the cydippid larvae reached approximately 5 mm in length (day 14). The cydippid larvae were then transferred to a circular tank with a water spray chamber. Approximately 21 days after the early development stage, cydippid larvae had grown to lengths of approximately 20 mm and were then bred in the same manner up to the 5th generation. The greatest advantages of this culture method are that it is less cumbersome and only requires feeding and observation until the cydippid larvae attain a size that enables them to pursue live Artemia nauplii. Thus, damage to the living body due to water exchange and the labor involved are reduced, thereby allowing stable breeding of B. mikado.

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Stable Laboratory Culture System for the Ctenophore <em>Mnemiopsis leidyi</em>

Cited by 3 publications

References 37 publications

Evolutionary origin of the nervous system from Ctenophora prospective

Evolutionary origin of the nervous system from Ctenophora prospective

Optimized husbandry and targeted gene-editing for the cnidarianNematostella vectensis

An effective method to mass culture a lobate ctenophore (<i>Bolinopsis mikado</i>)

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