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Cited by 30 publications
(56 citation statements)
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“…This finding may be reasoned by the reduced in vitro expression of Stx2B by recombinant B. subtilis cells and the relatively low sensitivity of the detection method. Under our experimental conditions Stx2B is expressed by vegetative cells in amounts lower than previously detected with the ETEC LTB (16,17). The anti-Stx2B sera employed to monitor the antigen in bacterial extracts detected a minimum of 120 ng of Stx2B toxin, while other sera, such as those previously used to quantify LTB, had a higher sensitivity (16,17).…”
Section: Discussionmentioning
confidence: 55%
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“…This finding may be reasoned by the reduced in vitro expression of Stx2B by recombinant B. subtilis cells and the relatively low sensitivity of the detection method. Under our experimental conditions Stx2B is expressed by vegetative cells in amounts lower than previously detected with the ETEC LTB (16,17). The anti-Stx2B sera employed to monitor the antigen in bacterial extracts detected a minimum of 120 ng of Stx2B toxin, while other sera, such as those previously used to quantify LTB, had a higher sensitivity (16,17).…”
Section: Discussionmentioning
confidence: 55%
“…Bacillus subtilis strains have been engineered to express different antigens as live recombinant vaccine vehicles delivered via the oral route either as spores or vegetative cells (5,6,8,16,17,21). Previous attempts to express the B subunit of heat labile toxin (LTB), produced by enterotoxigenic E. coli (ETEC) strains, have demonstrated that orally delivered B. subtilis vaccine strains may elicit protective systemic antibody responses to a lethal toxin challenge in mice (17).…”
Section: Shiga-like Toxin (Stx)-producing Enterohaemorragicmentioning
confidence: 99%
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