Stabilization of thymidine phosphorylase from Escherichia coli by immobilization and post immobilization techniques

Immobilized Drosophila melanogaster Deoxyribonucleoside Kinase (DmdNK) as a High Performing Biocatalyst for the Synthesis of Purine Arabinonucleotides

“…[32,33] Initially, the experiments were performed by loading a low amount of enzyme on the carrier (0.1-0.5 mg g…”

“…[33] Preparation of Aldehyde Dextran Dextran (1.67 g) was suspended in deionized water (50 mL) and NaIO 4 (0.43 g) was added to obtain 10% of oxidation degree. The reaction was carried out for 2 h at room temperature and then the solution was dialyzed (3500 Da MWCO) against deionized water.…”

“…This process permits one to obtain a multisubunit immobilization. [32,33] Furthermore, in some cases, enzyme properties have been enhanced upon immobilization. [34] The present work describes the study of substrate specificity of DmdNK from the standpoint of its application as a biocatalyst, its immobilization on a solid support and the use of the resulting biocatalyst for the preparative synthesis of araA-MP and FaraA-MP.…”

Development of an immobilized biocatalyst based on Bacillus psychrosaccharolyticus NDT for the preparative synthesis of trifluridine and decytabine

“…Immobilization of BpNDT by direct covalent reaction with aldehyde activated carriers caused a complete loss of the enzyme activity. Results were slightly improved using the approach already described for other multimeric enzymes: adsorption on a strong anionic exchange carrier (agarose coated with PEI) followed by crosslinking with aldehydedextran [8][9][10].…”

“…These enzymes are used to catalyze, in presence of inorganic phosphate, the transfer of the sugar moiety from a sugar donor nucleoside to a sugar acceptor base leading to the production of a second nucleoside through a two-step reaction. Moreover, several NPs from different sources have been also conveniently immobilized by following various approaches and obtaining, in most of cases, robust biocatalysts for preparative reactions [5,[8][9][10]. However, the poor ability of NPs to recognize cytosine derivatives as substrates [11] and, often, the need to follow a two-enzyme one-pot scheme (PyNP-PNP or UP-PNP) to synthesize purine nucleosides, represent the main obstacle to their broad application.…”