Some of the variables influencing the stability of purified and unpurified spinach catalase during storage were evaluated in model systems. A purified preparation of spinach catalase was obtained from spinach leaves by comminution and extraction with phosphate buffer and extraction with n‐butanol, NH4)2SO4 precipitation, (NH4)2SO4 fractionation, dialysis, and lyophilization. The preparation had a Kat.‐f. of 3300 at pH 7.0. The stability of the enzyme in storage was influenced primarily by storage tempe…
scite is a Brooklyn-based startup that helps researchers better discover and evaluate scientific articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by researchers from dozens of countries and is funded in part by the National Science Foundation and the National Institute of Drug Abuse of the National Institutes of Health.