Stability of membrane potential in heart mitochondria: Single mitochondrion imaging

Detection of swelling of single isolated mitochondrion with optical microscopy

“…For microscopic measurements, mitochondria were adsorbed onto a glassbottom culture dish (35 mm in diameter) at approximately 10 6 mitochondria/dish. Mitochondria were then incubated for 90 min in an isotonic solution (10 mM Tris-HCl, 250 mM sucrose, 1 mM EGTA, 1 mM KH 2 PO 4 , pH 7.4) and were washed twice before microscopic measurements [15]. Adsorption and washing were performed at 4°C.…”

Toxicity of polyhydroxylated fullerene to mitochondria

“…In normal conditions, the existing proton gradient between the inner and outer membrane creates a membrane potential that allows for efficient phosphorylation of ADP, producing enough ATP to support K + entering into mitochondria [51]. Changes of potential gradient across the membrane of phospholipid bilayer are accompanied by appreciable modifications of the lipid bilayer rigidity and thickness, the conformation of membrane proteins, and the accessibility of reactive chemical groups.…”

Isolation of mitochondria by gentle cell membrane disruption, and their subsequent characterization

“…The membrane potential of isolated mitochondria was measured using fluorescence microscopy; mitochondria were stained with tetramethylrhodamine ethyl ester (TMRE), a potentiometric fluorescent dye [12]. Before the measurements, mitochondria adsorbed onto a glass bottom dish were incubated with 50 nM TMRE for 10 min at room temperature.…”