SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype

İpek, Ahmet; Barut, Erdoğan; Gülen, Hatice; Öz, Ayşe Tülin; Tangu, Nesrin Aktepe; İpek, Meryem

doi:10.4238/vol8-4gmr659

Cited by 14 publications

(13 citation statements)

References 14 publications

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“…Among the 4 loci, the EMO3 locus (H E = 0.83) showed the highest value of genetic diversity while the lowest diversity value was found using primer EMO2 (H E = 0.68), with a mean value of 0.75. In accord with prior findings (Diaz et al, 2006), our high heterozygosity values were similar to those of several studies that used SSR markers on olive cultivars in Italian regions as such Sicily (La Mantia et al, 2005) and Emilia (Ganino et al, 2007), of the Istria region in Croatia (Poljuha et al, 2008) as well as in the southern Marmara region in Turkey (Ipek et al, 2009). Also, the mean observed heterozygosity values (H O = 0.78) of the present study were higher than expected (H E = 0.75) and in general higher than previous studies using cultivars from different areas of the Mediterranean basin (Sarri et al, 2006).…”

Section: Ssr Polymorphismsupporting

confidence: 92%

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Delgado-Martinez¹,

Amaya²,

Sánchez-Sevilla³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8, with a mean of 6.25 alleles per primer pair (a total of 25 alleles). The observed heterozygosity ranged from 0.58 to 0.95, while the expected heterozygosity varied between 0.68 and 0.83. The polymorphism information content values ranged from 0.63 to 0.79. The mean polymorphism information content value of 0.70 for the SSR loci provided sufficient discriminating ability to evaluate the genetic diversity among the cultivars. The SSR data allowed unequivocal identification of all the cultivars; a combination of three SSR markers was sufficient to discriminate all 25 olive cultivars. A dendrogram was prepared, using the unweighted pair-group method with arithmetic mean clustering algorithm; it depicted the pattern of Genetic diversity of olive cultivars by microsatellite markers relationships between the cultivars. Most of the local cultivars grouped according to their geographic origin. No clear clustering trends were observed when the morphological traits of fruit endocarps or fruit use of cultivars were employed as analysis criteria. We conclude that there is a high level of variability among local olive cultivars from the Extremadura region at both the morphological and molecular levels; these data should be useful for identifying and distinguishing local germplasm.

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Section: Ssr Polymorphismsupporting

confidence: 92%

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Delgado-Martinez¹,

Amaya²,

Sánchez-Sevilla³

et al. 2012

Genet. Mol. Res.

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“…Later, amplified fragment length polymorphism (AFLP) markers were found to be useful for the assessment of genetic diversity between olive cultivars, wild genotypes, and Olea species (Angiolillo et al, 1999;Owen et al, 2005;Ercisli et al, 2009). Simple sequence repeat (SSR) markers are another choice of marker technology for the characterization of olive genotypes and a considerable number of SSR markers have been developed for the olive genome in recent years (e.g., Rallo et al, 2000;Sefc et al, 2000;Ipek et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

et al. 2015

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ABSTRACT. The purpose of this study was to characterize olive core collection with amplified fragment length polymorphism (AFLP) markers and fruit traits and to determine AFLP markers significantly associated with these fruit characters in olive. A total of 168 polymorphic AFLP markers generated by five primer combinations and nine fruit traits were used to characterize relationships between 18 olive cultivars. Although all olive cultivars were discriminated from each other by either AFLP markers (<0.75 similarity level) or fruit traits, clustering based on the AFLP markers and fruit traits was not significantly correlated (r = 0.13). Partial clustering of olive cultivars by AFLP markers according to their geographical origin was observed. Associations of AFLP markers with fruits were determined using a multiple-regression analysis with stepwise addition of AFLP markers. Significant associations between eight AFLP markers and fruit traits were identified. While five AFLP markers demonstrated significant negative correlation with fruit and stone weight, width and length and total polyphenols (P < 0.05), three AFLP markers displayed significant positive correlation with α-tocopherol and γ-tocopherol (P < 0.01). This is the first report on the association of molecular markers with fruit traits in olive. Molecular markers associated with morphological and agronomic traits could be utilized for the breeding of olive cultivars. However, the association power of these markers needs to be confirmed in larger populations, and highly correlated markers should then be converted to PCR-based DNA markers such as sequence-characterized amplified region markers for better utilization.

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“…The observed heterozygosity (Ho) was higher than the expected heterozygosity (He) for DCA-11, DCA-16, DCA-17 and GAPU-89, while the Ho was lower than the He for DCA-04, DCA-09 and UDO-14 ( The names in parentheses are synonyms given by the local growers; † a number was given to each sample to prevent confusion because more than one sample was collected for the most of cultivars. SSR markers have been developed for olives by several research groups (Carriero et al, 2002;Cipriani et al, 2002;Sefc et al, 2000), and this marker system was found to be the most reliable, effective and easy-to-use for cultivar identification in olives (Baldoni et al, 2009;Ipek et al, 2009;Muzzalupo et al, 2010;Sarri et al, 2006). In some studies, polyacrylamide gels and DNA analysis systems with fluorescent labeling were used for detecting polymorphic alleles of SSR markers (Baldoni et al, 2009;Carriero et al, 2002;Gomes et al, 2008;Sarri et al, 2006).…”

Section: Ssr Polymorphisms Among Olive Cultivarsmentioning

confidence: 99%

“…Morphological and pomological characteristics of common Turkish olive cultivars were previously determined by Canozer (1991). Genetic variation among some Turkish olive cultivars was analyzed using DNA based molecular markers (Owen et al, 2005;Ozkaya et al, 2006;Ercisli et al, 2009;Ipek et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Assessment of inter- and intra-cultivar variations in olive using SSR markers

İpek

Barut

Gülen

et al. 2012

Sci. agric. (Piracicaba, Braz.)

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Olive (Olea europaea L.) production in the world has been made by using many cultivars, and the genetic uniformity of commercial cultivars is important for standard olive oil and table olive production. The genetic variation among and within commonly cultivated olive cultivars in Turkey was analyzed using SSR markers. A total of 135 leaf samples were collected from 11 commonly cultivated olive cultivars from 11 provinces in four geographical regions of Turkey. Seven SSR primer pairs generated 46 SSR markers, and the number of SSR markers per primer pair ranged from 4 (UDO-14) to 9 (GAPU-89) with an average of 6.57. This high level of SSR polymorphism suggests that olive production in Turkey has been made using genetically diverse olive cultivars and this high level of genetic variation is probably due to the location of However, there was some confusion about the identification of cultivars with similar phenotypic traits. To prevent misidentification of olive cultivars and to minimize intra-cultivar variation, certified propagation materials which were characterized using DNA based molecular markers should be used during the establishment of new olive orchards.

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SSR analysis demonstrates that olive production in the southern Marmara region in Turkey uses a single genotype

Cited by 14 publications

References 14 publications

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain

Identification of molecular markers associated with fruit traits in olive and assessment of olive core collection with AFLP markers and fruit traits

Assessment of inter- and intra-cultivar variations in olive using SSR markers

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