Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of Sclerotinia sclerotiorum

Yu, Yang; Xiao, Jifen; Yong, Yang; Bi, Chaowei; Qing, Ling; Wan-zhong, Tan

doi:10.1016/j.pmpp.2015.04.005

Cited by 33 publications

(20 citation statements)

References 51 publications

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“…In addition to the well-known effectors that have been mentioned above, a number of genes encoding other S. sclerotiorum effectors, including SsBi1 [20], SsCaf1 [25], SCat1 [18], SsSodI [19] and SsPemG1 [29] were expressed, but not significantly induced in the current study. The discrepancy might be due to host and isolate-dependent differences in the expression of these genes or differences in experimental design.…”

Section: Discussionmentioning

confidence: 49%

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Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

et al. 2017

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Background Sclerotinia sclerotiorum causes stem rot in Brassica napus, which leads to lodging and severe yield losses. Although recent studies have explored significant progress in the characterization of individual S. sclerotiorum pathogenicity factors, a gap exists in profiling gene expression throughout the course of S. sclerotiorum infection on a host plant. In this study, RNA-Seq analysis was performed with focus on the events occurring through the early (1 h) to the middle (48 h) stages of infection.ResultsTranscript analysis revealed the temporal pattern and amplitude of the deployment of genes associated with aspects of pathogenicity or virulence during the course of S. sclerotiorum infection on Brassica napus. These genes were categorized into eight functional groups: hydrolytic enzymes, secondary metabolites, detoxification, signaling, development, secreted effectors, oxalic acid and reactive oxygen species production. The induction patterns of nearly all of these genes agreed with their predicted functions. Principal component analysis delineated gene expression patterns that signified transitions between pathogenic phases, namely host penetration, ramification and necrotic stages, and provided evidence for the occurrence of a brief biotrophic phase soon after host penetration.ConclusionsThe current observations support the notion that S. sclerotiorum deploys an array of factors and complex strategies to facilitate host colonization and mitigate host defenses. This investigation provides a broad overview of the sequential expression of virulence/pathogenicity-associated genes during infection of B. napus by S. sclerotiorum and provides information for further characterization of genes involved in the S. sclerotiorum-host plant interactions.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3642-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 49%

“…Catalase ( SCat1 ) [18] and superoxide dismutase ( SsSodI ) [19] appear to modulate the deleterious effects of these compounds internally. The apoptosis inhibitor, BAX inhibitor-1 ( SsBI1 ), is also required for full virulence and was postulated to prevent hyphal apoptosis resulting from exposure to host-derived ROS [20]. …”

Section: Introductionmentioning

confidence: 99%

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

et al. 2017

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“…The wild-type strains were inoculated on the PDA medium and Arabidopsis thaliana as described by Yu et al (2015) followed by mycelia harvest. To compare the expression level of SsXly1 on different carbon sources, the wild-type strains were cultured on different minimum medium in which the glucose was replaced with xylan or tomato leaf extract.…”

Section: Methodsmentioning

confidence: 99%

Disruption of the Gene Encoding Endo-β-1, 4-Xylanase Affects the Growth and Virulence of Sclerotinia sclerotiorum

Xiao

et al. 2016

Front. Microbiol.

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Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plant’s cell-wall components. Functional analyses of the genes that encode CWDEs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-β-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-β-1, 4-xylanase in the growth and virulence of S. sclerotiorum.

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“…BAX inhibitor-1 (Bi1) is a highly conserved protein that is found in both eukaryotes and prokaryotes, exhibiting apoptosis-inhibiting activity. RNAi knockdown mutants in S. sclerotiorum showed more aerial hyphal growth and reduced virulence [48]. The mutants also exhibit reduced tolerance to chemical stress, as its gene expression is induced by various stresses.…”

Section: Regulation Of Mycelial Growth and Virulencementioning

confidence: 99%

The Notorious Soilborne Pathogenic Fungus Sclerotinia sclerotiorum: An Update on Genes Studied with Mutant Analysis

Xia

Hoy

et al. 2019

Pathogens

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Ascomycete Sclerotinia sclerotiorum (Lib.) de Bary is one of the most damaging soilborne fungal pathogens affecting hundreds of plant hosts, including many economically important crops. Its genomic sequence has been available for less than a decade, and it was recently updated with higher completion and better gene annotation. Here, we review key molecular findings on the unique biology and pathogenesis process of S. sclerotiorum, focusing on genes that have been studied in depth using mutant analysis. Analyses of these genes have revealed critical players in the basic biological processes of this unique pathogen, including mycelial growth, appressorium establishment, sclerotial formation, apothecial and ascospore development, and virulence. Additionally, the synthesis has uncovered gaps in the current knowledge regarding this fungus. We hope that this review will serve to build a better current understanding of the biology of this under-studied notorious soilborne pathogenic fungus.

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Ss-Bi1 encodes a putative BAX inhibitor-1 protein that is required for full virulence of Sclerotinia sclerotiorum

Cited by 33 publications

References 51 publications

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus

Disruption of the Gene Encoding Endo-β-1, 4-Xylanase Affects the Growth and Virulence of Sclerotinia sclerotiorum

The Notorious Soilborne Pathogenic Fungus Sclerotinia sclerotiorum: An Update on Genes Studied with Mutant Analysis

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