Spread of OXA-48 and NDM-1-Producing Klebsiella pneumoniae ST48 and ST101 in Chicken Meat in Western Algeria

Abstract

We investigated the prevalence of carbapenemase-producing Enterobacteriaceae (CPE) in chicken meat in Western Algeria in 2017. Results: From February to July 2017, samples of chicken meat from three poultry farms in Western Algeria were screened for the presence of CPE. Strains were characterized with regard to antibiotic resistance, blactamase content, Plasmid-mediated quinolone resistance, sulfonamide resistance genes, clonality (repetitive sequence-based profiles and multilocus sequence typing) and virulence traits. Of 181 samples analyzed, 29 (16.0%) carbapenemase-producing Klebsiella pneumoniae were detected. Twenty-three OXA-48-producers (79.3%) and six (20.7%) New Delhi metallo (NDM)-1-producers were observed. Clonality analysis showed three distinct lineages and clonal expansions of the OXA-48-producing K. pneumoniae ST48 and the NDM-1producing K. pneumoniae ST101. These isolates harbored fimH, ureA, mrkD, entB, uge, and wabG. Neither capsular serotype genes nor hypermucoviscous phenotype were detected. Plasmid analysis confirmed that all these isolates harbored the transferable IncL and IncFIIK plasmids. Conclusions: This study reports the spread of OXA-48 and NDM-1-producing K. pneumoniae ST48 and ST101 in chicken meat in Western Algeria and demonstrates that food represents a reservoir of the carbapenemases encoding genes.

Whole-Genome Sequencing of Gram-Negative Bacteria Isolated From Bovine Mastitis and Raw Milk: The First Emergence of Colistin mcr-10 and Fosfomycin fosA5 Resistance Genes in Klebsiella pneumoniae in Middle East

“…On the other hand, WGS analysis of Klebsiella species showed that K. pneumoniae had a novel ST, that was closely matched to ST1224, and ST48 alleles. In earlier studies, K. pneumoniae ST1224 was isolated from neonates ( Chen et al, 2019 ), while K. pneumoniae ST48 was reported in chicken meat in Western Algeria, one of the Middle East region ( Chaalal et al, 2021 ) indicating that these STs are not host specific and could be easily transmitted to humans from food animals and their products.…”

Diversity in the Characteristics of Klebsiella pneumoniae ST101 of Human, Environmental, and Animal Origin

“…In addition to reports of invasive infections, contamination of food animals or food products with MDR K. pneumoniae has been reported ( Davis and Price, 2016 ; Wu et al, 2016 ; Projahn et al, 2019 ). Recently, the presence of NDM-1 carbapenemase-producing ST101 K. pneumoniae has been reported in chicken meat in Algeria ( Chaalal et al, 2020 ). Likewise, other high-risk MDR clones, such as ST11 and ST258, have been detected in animals in China ( Yang et al, 2019 ).…”

Fighting Antibiotic Resistance in Hospital-Acquired Infections: Current State and Emerging Technologies in Disease Prevention, Diagnostics and Therapy

“…Although ARGs existed long before antibiotics became discovered and put into a wide use (Hall and Barlow, 2004;Allen et al, 2010), our irresponsible use of antibiotics in animal husbandry, overuse of antibiotics in health care, improper treatment of wastewaters and poor sanitation in low and middle income countries has led to wide spread of resistant bacteria across the globe (Prestinaci et al, 2015;Holmes et al, 2016;Ma et al, 2020). To date, the most worrisome global spread of ARGs is the plasmid-mediated spread of carbapenemases (KPC, NDM, VIM, OXA-48, and OXA-51); colistin-resistance genes (mcr) in Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa; vancomycin resistance gene (vanA) in Enterococci and Staphylococcus aureus; and methicillin resistance gene (mecA) in S. aureus (de Niederhausern et al, 2011;Poirel et al, 2014;Lerner et al, 2015;Wang R. et al, 2018;Chaalal et al, 2020;Hishinuma et al, 2020;Snitser et al, 2020).…”

“…To date, the most worrisome global spread of ARGs is the plasmid-mediated spread of carbapenemases (KPC, NDM, VIM, OXA-48, and OXA-51); colistin-resistance genes ( mcr ) in Enterobacteriaceae , Acinetobacter baumannii , and Pseudomonas aeruginosa ; vancomycin resistance gene ( vanA ) in Enterococci and Staphylococcus aureus ; and methicillin resistance gene ( mecA ) in S . aureus ( de Niederhausern et al, 2011 ; Poirel et al, 2014 ; Lerner et al, 2015 ; Wang R. et al, 2018 ; Chaalal et al, 2020 ; Hishinuma et al, 2020 ; Snitser et al, 2020 ).…”