SPR screening of metal chelating peptides in a hydrolysate for their antioxidant properties

Canabady-Rochelle, Laetitia; Selmeczi, Katalin; Collin, Sabrina; Pasc, Andreea; Muhr, Laurence; Boschi‐Muller, Sandrine

doi:10.1016/j.foodchem.2017.06.116

Cited by 40 publications

(42 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antioxidant capacity that was determined by Cu(II) chelation was measured by spectrophotometry while using murexide as a colorimetric indicator. This test was adapted from literature, as described in ref [55]. In the presence of a copper(II)-chelating sample, the copper-murexide complex dissociates, then forms free murexide in solution and a chelator-copper complex.…”

Section: Copper(ii)-chelating Activitymentioning

confidence: 99%

“…In a first set of experiments, we evaluated the Fe(II) and the Cu(II) chelating ability of GQDs and compared the results with those that were obtained while using EDTA and carnosine, compounds that are well-known to exhibit metal chelating properties [53][54][55][56][57]. GQDs, used at various concentrations, were added to a 2 mM solution and FeCl 2 and the mixture was incubated for 3 min at room temperature.…”

Section: Fe(ii) and Cu(ii) Chelating Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

et al. 2020

View full text Add to dashboard Cite

2,2’-(Ethylenedioxy)bis(ethylamine)-functionalized graphene quantum dots (GQDs) were prepared under mild conditions from graphene oxide (GO) via oxidative fragmentation. The as-prepared GQDs have an average diameter of ca. 4 nm, possess good colloidal stability, and emit strong green-yellow light with a photoluminescence (PL) quantum yield of 22% upon excitation at 375 nm. We also demonstrated that the GQDs exhibit high photostability and the PL intensity is poorly affected while tuning the pH from 1 to 8. Finally, GQDs can be used to chelate Fe(II) and Cu(II) cations, scavenge radicals, and reduce Fe(III) into Fe(II). These chelating and reducing properties that associate to the low cytotoxicity of GQDs show that these nanoparticles are of high interest as antioxidants for health applications.

show abstract

Section: Copper(ii)-chelating Activitymentioning

confidence: 99%

Section: Fe(ii) and Cu(ii) Chelating Propertiesmentioning

confidence: 99%

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Several regeneration buffers were prepared as in Canabady‐Rochelle et al. [19]: 500 mM imidazole solution, a running buffer (10 mL of PBS 1×, 0.005% Tween 20, pH 7.4, containing 87 µL EDTA at 350 mM) and 0.5% SDS solution. Both imidazole and SDS solutions were prepared in ultrapure water.…”

Section: Methodsmentioning

confidence: 99%

“…The SPR experiments were led similarly as in Canabady‐Rochelle et al. [19] and reported briefly. The binding affinity of peptides and imidazole to Ni 2+ was analyzed by SPR at 25°C using a Biacore X100 instrument (GE Healthcare, Uppsala, Sweden) equipped with NTA sensor chips.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Chromatographic separation simulation of metal‐chelating peptides from surface plasmon resonance binding parameters

Muhr

Pontvianne

Selmeczi

et al. 2020

J of Separation Science

Self Cite

View full text Add to dashboard Cite

Funding information LUE IMPACT BiomoleculesSome metal-chelating peptides have antioxidant properties, with potential nutrition, health, and cosmetics applications. This study aimed to simulate their separation on immobilized metal ion affinity chromatography from their affinity constant for immobilized metal ion determined in surface plasmon resonance, both technics are based on peptide-metal ion interactions. In our approach, first, the affinity constant of synthetic peptides was determined by surface plasmon resonance and used as input data to numerically simulate the chromatographic separation with a transport-dispersive model based on Langmuir adsorption isotherm. Then, chromatographic separation was applied on the same peptides to determine their retention time and compare this experimental t R with the simulated t R obtained from simulation from surface plasmon resonance data. For the investigated peptides, the relative values of t R were comparable. Hence, our study demonstrated the pertinence of such numerical simulation correlating immobilized metal ion affinity chromatography and surface plasmon resonance. K E Y W O R D Schelation, immobilized metal ion affinity chromatography, modeling, peptides, surface plasmon resonance

show abstract

Metal‐chelating antioxidant peptides: Biosensor screening methods as alternatives to the ferrozine assay

Bjørlie,

Irankunda,

Yesiltas

et al. 2024

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Preventing metal‐catalyzed lipid oxidation in food products, which decreases nutritional value and sensory quality, is crucial in the food industry. This is typically achieved through the use of metal‐chelating molecules. While the ferrozine assay is widely used to screen protein hydrolysates for metal chelating activity, it has proven difficult to use with pure peptides. This study evaluates the potential of surface plasmon resonance (SPR) and electrically switchable nanolever technology (switchSENSE®) as alternative screening methods. Unfortunately, solubility issues and large standard deviations precluded a direct correlation between the ferrozine assay and these biosensor techniques. Both techniques, however, were able to quantitatively distinguish between two peptides with very similar sequences despite the absence of a correlation between dissociation constants determined by SPR and switchSENSE®. This study highlights the potential of SPR and switchSENSE® for screening the metal chelating activity of pure peptides, advancing the understanding of peptide‐metal ion interactions.

show abstract

SPR screening of metal chelating peptides in a hydrolysate for their antioxidant properties

Cited by 40 publications

References 37 publications

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

One-Step Synthesis of Diamine-Functionalized Graphene Quantum Dots from Graphene Oxide and Their Chelating and Antioxidant Activities

Chromatographic separation simulation of metal‐chelating peptides from surface plasmon resonance binding parameters

Metal‐chelating antioxidant peptides: Biosensor screening methods as alternatives to the ferrozine assay

Contact Info

Product

Resources

About