Spontaneous formation of vesicles

Šegota, Suzana; Težak, Đurđica

doi:10.1016/j.cis.2006.01.002

Cited by 248 publications

(225 citation statements)

References 317 publications

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“…see reviews [10,11]). In this context, mixtures of oppositely charged surfactants have emerged in the last 10-15 years as an exciting new class of vesicle-forming systems (catanionic vesicles), as described further [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Brito

Marques

Silva

et al. 2009

Colloids and Surfaces B: Biointerfaces

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a b s t r a c tThe design of efficient liposomal systems for drug delivery is of considerable biomedical interest. In this context, vesicles prepared from cationic/anionic surfactants may offer several advantages, mainly due to their spontaneity in formation and long-term stability. There is also an impending need to produce less toxic, more biocompatible amphiphiles, while maintaining the desirable aggregation properties. In this work, we present data for acute toxicity to Daphnia magna (IC 50 ), and potential ocular irritation (HC 50 ) for some newly prepared ionic surfactants with dodecyl chains, derived from the amino acids tyrosine (Tyr), serine (Ser), hydroxyproline (Hyp) and lysine (Lys). The micellization behavior of the compounds, evaluated from surface tension measurements, is presented and compared to more conventional ionic amphiphiles. Two types of spontaneouly formed catanionic vesicles, composed either by a dodecyltrimethylammonium bromide (DTAB)/Lys-derivative and or Ser-/Lys-derivative mixture, have also been tested for their ecotoxicity and hemolytic potential. All the micelle-forming surfactants as well as the vesicle-containing mixtures are found to have lower ecotoxicity than the reference surfactant DTAB. Moreover, the results from hemolysis and hemoglobin denaturation tests show that the Tyr-and Lys-derivatives are moderately irritant, whereas the Hyp-and Ser-ones are just slightly irritant. Even more significantly, the vesicle-containing mixtures exhibit lower hemolytic activity than the neat surfactants, a positive result for their potential use in liposomal formulations.

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Section: Introductionmentioning

confidence: 99%

Physicochemical and toxicological properties of novel amino acid-based amphiphiles and their spontaneously formed catanionic vesicles

Brito

Marques

Silva

et al. 2009

Colloids and Surfaces B: Biointerfaces

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“…These are very elegant objects that allow manipulation and real time observation with a light microscope [37][38][39][40] and that have opened the way to a wealth of theoretical and experimental investigations. 41 However, unlike experimental work on giant unilamellar vesicles, experimental reports on real-time observation of the effect of a chemical species on the stability and shape changes of a multilamellar vesicles are extremely scarce. [42][43][44][45][46] Nevertheless, as it is demonstrated in this paper, when multilamellar vesicles are used, richer behaviours can be expected, since cooperative effects due to the dense packing of bilayers may play an important role.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Observation of Polyelectrolyte-Induced Binding of Charged Bilayers

Luan

Ramos

2007

J. Am. Chem. Soc.

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Abstract:We present real-time observations by confocal microscopy of the dynamic behavior of multilamellar vesicles (MLVs), composed of charged synthetic lipids, when put in contact with oppositely charged polyelectrolyte (PE) molecules. We find that the MLVs exhibit astonishing morphological transitions, which result from the discrete and progressive binding of the charged bilayers induced by a high PE concentration gradient. Our physical picture is confirmed by quantitative measurements of the fluorescence intensity as the bilayers bind to each other. The shape transitions lead eventually to the spontaneous formation of hollow capsules, whose thick walls are composed of lipid multilayers condensed with PE molecules. This class of objects may have some (bio)technological applications.

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“…Methods of preparing of polymeric nanoparticles have been reviewed 15, 61 and they include ionic gelation, coacervation, solvent evaporation, spontaneous emulsification/solvent diffusion, salting out/emulsification-diffusion, supercritical fluid technology and polymerization. Depending on the materials utilized, such as phospholipids and glycolipids, the desired liposome structure can be prepared by sonication, electroformation, extrusion from diluted lamellar dispersions, high-shear homogenization, reverse-phase evaporation, gel exclusion chromatography, freezelyophilization, calcium-induced fusion, detergent dialysis and ultracentrifugation [62][63][64][65] .…”

Section: Methods Of Fabricationmentioning

confidence: 99%