Split-gene system for hybrid wheat seed production

Abstract

Hybrid wheat plants are superior in yield and growth characteristics compared with their homozygous parents. The commercial production of wheat hybrids is difficult because of the inbreeding nature of wheat and the lack of a practical fertility control that enforces outcrossing. We describe a hybrid wheat system that relies on the expression of a phytotoxic barnase and provides for male sterility. The barnase coding information is divided and distributed at two loci that are located on allelic positions of the host chromosome and are therefore "linked in repulsion." Functional complementation of the loci is achieved through coexpression of the barnase fragments and intein-mediated ligation of the barnase protein fragments. This system allows for growth and maintenance of male-sterile female crossing partners, whereas the hybrids are fertile. The technology does not require fertility restorers and is based solely on the genetic modification of the female crossing partner.hybrid wheat breeding | split-gene approach | site-specific recombination | intein-mediated protein splicing

Genome-based establishment of a high-yielding heterotic pattern for hybrid wheat breeding

“…The latter is of particular relevance for climate-smart agriculture and low-yielding environments, where wheat is widely grown (4). Wheat hybrids are currently cultivated on only <1% of the global acreage, mainly because of the failure to implement a cost-efficient hybrid seed production system, which is required for establishment of a competitive hybrid breeding program (5). Recently, considerable progress has been achieved in developing alternative, more economically feasible hybridization systems, such as the functional characterization of potential cytoplasmic male sterility systems (6).…”

Review on Impact of Plant Breeding in Crop Improvement, Ethiopia

“…However, to permit potential high yield to be fully realized and maintained, disease must be controlled (Lupton, 2014). The applications of transgenic technology for wheat hybrid seed production become successful through application of transgenic technology for hybrid seed production in wheat (Kempe et al, 2014). Induced mutation is used with great success by different breeding programs for developing new cultivars.…”

“…Global food security demands the development of new technologies to increase and secure cereal production on finite arable land without increasing water and fertilizer use. The use of heterosis through hybrid breeding has produced tremendous economic benefits in worldwide crop production (Kempe et al, 2014).…”

Phylogenetic distribution of extracellular guanyl-preferring ribonucleases renews taxonomic status of two <i>Bacillus</i> strains

“…Barnase, an extracellular alkaline guanyl-preferring ribonuclease first isolated from Bacillus amyloliquefaciens (Hartley and Rogerson, 1972), was shown to possess a wide range of biological activities and is of special interest for research and practical applications in biotechnology and medicine (Kempe et al, 2014;Spång et al, 2012;Sreenivasan et al, 2013;Ulyanova et al, 2011). Ribonucleases similar to barnase (UniProtKB/Swiss-Prot accession number P00648) were purified from B. intermedius 7P (P00649), B. circulans BCF-256 (P35078), B. pumilus KMM-62 (P48068), B. coagulans BCF-247 (P37203), and B. thuringiensis var.…”

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† The GenBank accession number for the 16S rRNA gene sequence of Bacillus altitudinis B-388 is JX129389, for the rpoB gene sequence of Bacillus altitudinis B-388 is JX129391, for the 16S rRNA gene sequence of Bacillus pumilus 7P is JX129390 and for the rpoB gene sequence of Bacillus pumilus 7P is JX129392 correspondingly.None of the authors of this manuscript has any financial or personal relationship with other people or organizations that could inappropriately influence their work.

IntroductionBarnase, an extracellular alkaline guanyl-preferring ribonuclease first isolated from Bacillus amyloliquefaciens (Hartley and Rogerson, 1972), was shown to possess a wide range of biological activities and is of special interest for research and practical applications in biotechnology and medicine (Kempe et al, 2014;Spång et al, 2012;Sreenivasan et al, 2013;Ulyanova et al, 2011). Ribonucleases similar to barnase (UniProtKB/Swiss-Prot accession number P00648) were purified from B. intermedius 7P (P00649), B. circulans BCF-256 (P35078), B. pumilus KMM-62 (P48068), B. coagulans BCF-247 (P37203), and B. thuringiensis var.

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