Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of <i>S. pneumoniae</i>

Deniset, Justin; Surewaard, Bas G.J.; Lee, Woo Yong; Kubes, Paul

doi:10.1084/jem.20161621

Cited by 184 publications

(260 citation statements)

References 39 publications

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“…Perhaps neutrophils in rhesus macaques reach full maturation at another anatomical site. Several studies have provided evidence for this to occur in mice (34, 35). …”

Section: Resultsmentioning

confidence: 98%

Neutrophil progenitor populations of rhesus macaques

Weisgrau

Vosler

Pomplun

et al. 2018

Journal of Leukocyte Biology

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Captive-bred rhesus macaques of Indian origin represent one of the most important large animal models for infectious disease, solid organ transplantation and stem cell research. There is a dearth of information defining hematopoietic development, including neutrophil leukocyte differentiation in this species using multicolor flow cytometry. In the current study we sought to identify cell surface markers that delineate neutrophil progenitor populations with characteristic immunophenotypes. We defined four different post-mitotic populations based on their CD11b and CD87 expression pattern, and further refined their immunophenotypes using CD32, CD64, lactoferrin and myeloperoxidase as antigenic markers. The four subsets contained myelocyte, metamyelocyte, band, and segmented neutrophil populations. We compared our flow cytometry-based classification with the classical nuclear morphology-based classification. We found overlap of immunological phenotype between populations of different nuclear morphology and identified phenotypically different subsets within populations of similar nuclear morphology. We assessed the responsiveness of these populations to stimulatory signals such as LPS, fMPL or PMA, and demonstrated significant differences between human and rhesus macaque neutrophil progenitors. In this study we provided evidence for species-specific features of granulopoiesis that ultimately manifested in the divergent immunophenotypes of the fully differentiated segmented neutrophils of humans and rhesus macaques. Additionally, we found functional markers that can be used to accurately quantify neutrophil progenitors by flow cytometry. While these markers do not coincide with the classical nuclear-morphology-based grading they enable us to perform functional studies monitoring immunophenotypic markers.

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“…Perhaps neutrophils in rhesus macaques reach full maturation at another anatomical site. Several studies have provided evidence for this to occur in mice (34, 35). …”

Section: Resultsmentioning

confidence: 98%

Neutrophil progenitor populations of rhesus macaques

Weisgrau

Vosler

Pomplun

et al. 2018

Journal of Leukocyte Biology

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“…31 Neutrophil depletion was confirmed by flow cytometry in both the peritoneal lavage fluid and spleen of treated mice ( Figure 6B,C,E). To achieve this, we specifically depleted neutrophils in vivo using the well-established anti-Ly6G neutralization antibody or IgG2a isotype control prior to induction of endometriosis.…”

Section: Neutrophil Depletion Alters Local and Systemic Cytokinesmentioning

confidence: 81%

Neutrophil recruitment and function in endometriosis patients and a syngeneic murine model

et al. 2019

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Endometriosis is a chronic inflammatory, gynecological disease characterized by the presence of endometrial-like tissue lesions outside of the uterus. Neutrophils are elevated in the systemic circulation and peritoneal fluid of endometriosis patients; however, whether and how neutrophils contribute to endometriosis pathophysiology remain poorly understood. With emerging roles for neutrophils in chronic and sterile inflammatory conditions, we sought to provide in-depth characterization of neutrophil involvement in endometriosis. We demonstrate that neutrophils reside within patient endometriotic lesions and that patient lesions possess a microenvironment that may influence neutrophil recruitment and function. We also provide the first evidence that systemic circulating neutrophils from endometriosis patients display distinct transcriptomic differences compared neutrophils from healthy control subjects.Time course characterization of our syngeneic, immunocompetent mouse model of endometriosis revealed that neutrophils are rapidly recruited to the peritoneal environment early after endometriotic lesion establishment and remain present in murine lesions long term. In vivo neutrophil depletion altered the systemic and peritoneal immune microenvironment of mice with endometriosis as demonstrated by changes in pro-inflammatory and angiogenic mediators. Taken together, these findings highlight a novel role for neutrophils in early events such as angiogenesis and modulation of the local inflammatory environment associated with endometriosis pathogenesis. K E Y W O R D Sangiogenesis, endometrium, immune microenvironment, inflammation, leukocytes | 1559 SYMONS et al.

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“…They found that, during a systemic Streptococcus pneumoniae infection, most of the bacteria bypass the marginal zone macrophages and are caught by the red pulp macrophages. Mature Ly6G‐high neutrophils residing in the red pulp (that were observed to scan the tissue under basal conditions) were seen plucking the bacteria off the surface of the red pulp macrophages . A dramatic increase in neutrophils was also observed in the marginal zone by 24 h after infection, and interestingly, IVM showed that these neutrophils were grabbed right out of the bloodstream by the marginal zone macrophages.…”

Section: Systemic Infectionsmentioning

confidence: 97%

“…In a more recent study, Deniset et al . used IVM to investigate the coordinated response of splenic neutrophils, macrophages, and B cells to fight Streptococcus pneumoniae .…”

Section: Systemic Infectionsmentioning

confidence: 99%

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Unraveling the host's immune response to infection: Seeing is believing

Scott

Sarkar

Kratofil

et al. 2019

Journal of Leukocyte Biology

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It has long been appreciated that understanding the interactions between the host and the pathogens that make us sick is critical for the prevention and treatment of disease. As antibiotics become increasingly ineffective, targeting the host and specific bacterial evasion mechanisms are becoming novel therapeutic approaches. The technology used to understand host‐pathogen interactions has dramatically advanced over the last century. We have moved away from using simple in vitro assays focused on single‐cell events to technologies that allow us to observe complex multicellular interactions in real time in live animals. Specifically, intravital microscopy (IVM) has improved our understanding of infection, from viral to bacterial to parasitic, and how the host immune system responds to these infections. Yet, at the same time it has allowed us to appreciate just how complex these interactions are and that current experimental models still have a number of limitations. In this review, we will discuss the advances in vivo IVM has brought to the study of host‐pathogen interactions, focusing primarily on bacterial infections and innate immunity.

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Splenic Ly6Ghigh mature and Ly6Gint immature neutrophils contribute to eradication of S. pneumoniae

Cited by 184 publications

References 39 publications

Neutrophil progenitor populations of rhesus macaques

Neutrophil progenitor populations of rhesus macaques

Neutrophil recruitment and function in endometriosis patients and a syngeneic murine model

Unraveling the host's immune response to infection: Seeing is believing

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