SPINDLY, ERECTA, and Its Ligand STOMAGEN Have a Role in Redox-Mediated Cortex Proliferation in the Arabidopsis Root

Cui, Hongchang; Kong, Danyu; Wei, Pengcheng; Hao, Yueling; Torii, Keiko U.; Lee, Jin Suk; Li, Jie

doi:10.1093/mp/ssu106

Cited by 53 publications

(65 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The asterisks indicate statistically significant differences (***p<0.001; **p<0.01) Brackets represent standard errors over twenty replications (ten biological replicates, each with two pools of independent samples) for pGA20 and pPHOR, and two replications (i.e., two pools of samples) for wild-type control believed due to its additional roles in cytokinin and redox signaling, and consequent effects on plant root development, brassinosteroid synthesis, circadian rhythm and photoperiodic flowering, and abiotic stress response (Tseng et al 2004;Swain et al 2001;Shimada et al 2006;Filardo et al 2009;Qin et al 2011;Steiner et al 2012;Cui et al 2014). Studies of biomass allocation showed that the increase in biomass growth we observed was associated with preferential allocation to shoots.…”

Section: Discussionmentioning

confidence: 99%

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Viswanath

et al. 2015

Tree Genetics & Genomes

View full text Add to dashboard Cite

Overexpression of genes that modify gibberellin (GA) metabolism and signaling have been previously shown to produce trees with improved biomass production but highly disturbed development. To examine if more subtle types of genetic modification of GA could improve growth rate and modify tree architecture, we transformed a model poplar genotype (Populus tremula × P. alba) with eight genes, including two cisgenes (intact copies of native genes), four intragenes (modified copies of native genes), and two transgenes (from sexually incompatible species), and studied their effects under greenhouse and field conditions. In the greenhouse, four out of the eight tested genes produced a significant and often striking improvement of stem volume, and two constructs significantly modified the proportion of root or shoot biomass. Characterization of GA concentrations in the cisgenic population that had an additional copy of a poplar GA20-oxidase gene showed elevated concentrations of 13-hydroxylated GAs compared to wild-type poplars. In the field, we observed growth improvement for three of the six tested constructs, but it was significantly greater for only one of the constructs, a pRGL:GA20-oxidase intragene. The greenhouse and field responses were highly variable, possibly to due to cross-talk among the GA pathway and other stress response pathways, or due to interactions between the cisgenes and intragenes with highly similar endogenes. Our results indicate that extensive field trials, similar to those required for conventional breeding, will be critical to evaluating the value and pleiotropic effects of GA-modifying genes.

show abstract

Section: Discussionmentioning

confidence: 99%

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Viswanath

et al. 2015

Tree Genetics & Genomes

View full text Add to dashboard Cite

show abstract

“…3 To determine how SPY affects root development, we conducted transcript profiling with roots from spy-3 mutant and wild type plants. 2 This transcriptome analysis showed that genes involved in redox homeostasis are enriched among the genes whose expression is altered in the spy mutant. Consistent with this, we found that the spy mutant has an elevated level of reactive oxygen species (ROS).…”

mentioning

confidence: 97%

“…Our recent studies suggest that root cortex proliferation is a protective mechanism against abiotic stress. 2 The initial evidence suggesting this role for root cortex is derived from our studies of the spy mutant. In the root of oneweek-old wild-type seedlings of Arabidopsis thaliana, there is a single layer of cortex; in contrast, in the spy mutant, an additional layer of cortex is produced from the endodermis within 3 days of germination.…”

mentioning

confidence: 99%

“…2 No cortex proliferation could be induced in plants with a mutation in the ERECTA gene (the Ler ecotype or the er105 mutant), regardless of the concentration of hydrogen peroxide for the treatment. Interestingly, further studies showed that STOMA-GEN, but not other ERECTA ligands, is essential to this redoxmediated developmental response.…”

mentioning

confidence: 99%

“…4 However, this seems to be at odds with our recent observation that the spy mutant develops a shorter root in normal growth medium than the wild type and its root growth is reduced to a greater extent in salt-containing medium. 2 One plausible explanation for this paradox is that the extra cortex cell layer in the spy mutant root, along with its shorter length, causes reduction in salt uptake from the soil, thus maintaining a normal level of salt in the shoot. To test this hypothesis, we sprayed salt solution directly onto the leaves of the spy-3 mutant and wild type shoots.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Cortex proliferation in the root is a protective mechanism against abiotic stress

Cui

2015

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

Although as an organ the root plays a pivotal role in nutrient and water uptake as well anchorage, individual cell types function distinctly. Cortex is regarded as the least differentiated cell type in the root, but little is known about its role in plant growth and physiology. In recent studies, we found that cortex proliferation can be induced by oxidative stress. Since all types of abiotic stress lead to oxidative stress, this finding suggests a role for cortex in coping with abiotic stress. This hypothesis was tested in this study using the spy mutant, which has an extra layer of cortex in the root. Interestingly, the spy mutant was shown to be hypersensitive to salt and oxidizing reagent applied to the leaves, but it was as tolerant as the wild type to these compounds in the soil. This result lends support to the notion that cortex has a protective role against abiotic stress arising from the soil.

show abstract

An LTR retrotransposon insertion inside CsERECTA for an LRR receptor-like serine/threonine-protein kinase results in compact (cp) plant architecture in cucumber

et al. 2023

View full text Add to dashboard Cite

The compact (cp) phenotype in cucumber (Cucumis sativus L.) is an important plant architecture-related trait with a great potential for cucumber improvement. In this study, we conducted map-based cloning of the cp locus, identi ed and functionally characterized the candidate gene. Comparative microscopic analysis suggested that the short internode in the cp mutant is due to fewer cell numbers. Fine genetic mapping delimited cp into an 8.8-kb region on chromosome 4 harboring only one gene, CsERECTA (CsER) that encodes a leucine rich repeat receptor-like kinase. The insertion of a 5.5-kb insertion of along terminal repeat retrotransposon in the 22 nd exon resulted in loss-of-function of CsER in the cp plant. Spatiotemporal expression analysis in cucumber and CsER promoter-driven GUS assays in Arabidopsis indicated that CsER was highly expressed in the stem apical meristem and young organs, but the expression level was similar in the wild type and mutant cucumber plants. However, CsER protein accumulation was reduced in the mutant as revealed by western hybridization. The mutation in cp also did not seem to affect self-association of CsER for formation of dimers. Ectopic expression of CsER in Arabidopsis was able to rescue the plant height of the loss-of-function AtERECTA mutant, whereas the compact in orescence and small rosette leaves of the mutant could be partially recovered. Transcriptome pro ling in the mutant and wild type plants revealed hormone biosynthesis/or signaling, and photosynthesis pathways associated with CsER-dependent regulatory network. Our work from the present study provides new insights for the use of cp in cucumber breeding. HighlightsThe cucumber compact (cp) plant architecture is due to a loss-of-function mutation inside the CsERECTA gene for a leucine rich repeat receptor-like kinase (LRR-RLK), which is caused by insertion of an LTR retrotransposon in its 22 nd exon.

show abstract

SPINDLY, ERECTA, and Its Ligand STOMAGEN Have a Role in Redox-Mediated Cortex Proliferation in the Arabidopsis Root

Cited by 53 publications

References 65 publications

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Recombinant DNA modification of gibberellin metabolism alters growth rate and biomass allocation in Populus

Cortex proliferation in the root is a protective mechanism against abiotic stress

An LTR retrotransposon insertion inside CsERECTA for an LRR receptor-like serine/threonine-protein kinase results in compact (cp) plant architecture in cucumber

Contact Info

Product

Resources

About