Spinal cord decellularized matrix scaffold loaded with engineered basic fibroblast growth factor‐overexpressed human umbilical cord mesenchymal stromal cells promoted the recovery of spinal cord injury

Abstract

Spinal cord injury (SCI) will lead to irreversible damage of sensory and motor function of central nervous system, which seriously affects patient's quality of life. A variety of nerve engineering materials carrying various stem cells and cell growth factors had used to promote the repair of SCI, but they could not mimic the actual matric niche at spinal cord to promote cell proliferation and differentiation. Thus, developing novel biomaterial providing better niche of spinal cord is a new strategy to treat the severe SCI. In this study, we constructed porcine spinal cord decellularized matrix scaffold (SC-DM) with biocompatibility to load engineered basic fibroblast growth factoroverexpressing human umbilical cord mesenchymal stromal cells (bFGF-HUCMSCs) for treating SCI. The continuously released bioactive bFGF factors from grafted bFGF-HUCMSCs and three-dimensional niche by SC-DM promoted the differentiation of endogenous stem cells into neurons with nerve conduction function, leading a markedly motor function recovery of SCI. These results indicated that the functional bFGF-HUCMSCs/SC-DM scaffold provided more suitable matric niche for nerve cells, that would be a promising strategy for the clinical application of SCI.axon regeneration, basic fibroblast growth factor, human umbilical cord mesenchymal stromal cells, spinal cord decellularized matrix, spinal cord injury Grażyna Kami nska-WinciorWenli He and Chunying Shi contributed equally to this work.

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

“…As the physiological activities in organogenesis and tissue homeostasis, bFGF has been used to combine with dECM to build the composite scaffold [ 155 ]. For instance, Lin et al .…”

Biomedical Application of Decellularized Scaffolds

“…Spinal cord injury is a devastating injury that often results in paralysis, incontinence, and sexual dysfunction below the injury level. , However, due to the limited regeneration capacity of the spinal cord and the complex microenvironment, there is no effective treatment method at present. As a result, disability and mortality rates have risen dramatically. − Tissue engineering technology has great potential in the treatment of spinal cord injury …”

Hydrogel-based treatments for spinal cord injuries

“…The massive death of nerve cells and insufficient neurogenesis and angiogenesis hinder SCI repair. Currently, exogenous neurotrophic factors have great potential to promote SCI repair [ 152 ]. However, augmenting neurogenesis with exogenous nutrients or drugs to treat SCI remains challenging.…”